Ultrastructural specialization at the host-pathogen interface in rust-infected flax

Littlefield, Larry J.; Bracker, Charles E.

doi:10.1007/bf01282533

Cited by 118 publications

(48 citation statements)

References 28 publications

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“…A ring of highly osmiophilic wall material is seen at the point where the dissolution of the tip has occurred. This ring is reminiscent of the osmiophilic 'neck rings' found round haustorial necks of the rust fungi (Van Dyke and Hooker, 1969;Ehrlich and Ehrlich, 1971;Hardwick, Greenwood and Wood, 1971;Heath and Heath, 1971;Littlefield and Bracker, 1972;Rijo and Sargent 1974;Coffey, 1976;Heath, 1976;Harder, 1978;Harder et al, 1978;Allen, CofTey and Heath, 1979). This structure is thought by Little and Bracker to represent an abrupt transition from the wall of the penetration peg to the wall of the haustorium.…”

Section: Ultrastructure Of Mycorrhizas Of Monotropamentioning

confidence: 86%

AN ULTRASTRUCTURAL ANALYSIS OF THE DEVELOPMENT OF MYCORRHIZAS IN MONOTROPA HYPOPITYS L.

Duddridge

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1982

New Phytologist

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SUMMARYThe ultrastructural differentiation of mycorrhizas of the achlorophyllous plant Monotropa hypopitys was examined in relation to the stages of growth of the shoot. Plants were collected at regular intervals during the growing season and their roots were immediately excised and prepared for scanning and transmission electron microscopy. In addition to the presence of a fungal sheath and Hartig net of similar structure to those seen in ectomycorrhizas, the outer cortical cells frequently contain characteristic fungal intrusions. While not hreaching the host cell wall, these intrusions stimulate its invagination. The resulting structures, analogous to the transfer cells found in regions of intensive solute mobilization and transport, are produced in greatest numbers during the period of most rapid shoot extension. The relationship between their structure and possible function is discussed. The structure of mycorrhizas in Monotropa hypopitys is sufficiently distinct from that of arbutoid mycorrhizas, to warrant the creation of a new category which we term 'monotropoid'.

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Section: Ultrastructure Of Mycorrhizas Of Monotropamentioning

confidence: 86%

AN ULTRASTRUCTURAL ANALYSIS OF THE DEVELOPMENT OF MYCORRHIZAS IN MONOTROPA HYPOPITYS L.

Duddridge

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1982

New Phytologist

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“…avenae, it was found that the neck ring was intensely electron-opaque in glutaraldehyde-fixed, but unosmicated, unstained tissue. This suggested that the neck ring contains mineral-based electron scattering substances (Littlefield and Bracker, 1972). Electron probe X-ray analysis of the haustorial neck ring of P. coronata avenae indicated the presence of silicon and phosphorus (Heath and Allen, 1985).…”

Section: Discussionmentioning

confidence: 99%

“…The neck ring stains intensely after conventional preparation procedures for electron microscopy. Using the periodate-chromate-phosphotungstate (PACP) method for staining the plant plasmalemma, Littlefield and Bracker (1972) showed that the neck ring was unstained as compared to intense staining of remainder of the neck wall. This was attributed to the preferential extraction of the neck ring with periodic acid before the heavy metal stains were applied.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of the dikaryotic stage of anise rust, Puccinia pimpinellae (F. Strauss) Link.

Baka¹

2014

Afr. J. Microbiol. Res.

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“…Such invaginations, also called furrows or troughs, have since been identified in additional freeze fracture EM studies of S. cerevisiae (2)(3)(4)(5)(6) and other fungi (7)(8)(9)(10)(11)(12)(13).…”

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confidence: 96%

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

et al. 2015

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Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineagespecific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns.

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Ultrastructural specialization at the host-pathogen interface in rust-infected flax

Cited by 118 publications

References 28 publications

AN ULTRASTRUCTURAL ANALYSIS OF THE DEVELOPMENT OF MYCORRHIZAS IN MONOTROPA HYPOPITYS L.

AN ULTRASTRUCTURAL ANALYSIS OF THE DEVELOPMENT OF MYCORRHIZAS IN MONOTROPA HYPOPITYS L.

Ultrastructure of the dikaryotic stage of anise rust, Puccinia pimpinellae (F. Strauss) Link.

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

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