Ultrathin Polypeptide Multilayer Films for the Fabrication of Model Liquid/Liquid Electrochemical Interfaces

Cheng, Yufei; Corn, Robert M.

doi:10.1021/jp9920693

Cited by 77 publications

(120 citation statements)

References 37 publications

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“…We also observed a (PLL͞HA) 20 multilayer in which the 14th and 20th HA (HA 14 -TR and HA 20 -TR) and only the last PLL (PLL 20 -FITC) layers were labeled (see Fig. 1C).…”

Section: Optical Waveguide Lightmode Spectroscopy (Owls)mentioning

confidence: 71%

“…The dye-conjugated polyelectrolytes, HA-TR and PLL-FITC, were adsorbed in the same way at a certain stage of the buildup. As an example, (PLL͞HA) 19 -PLL 20 -FITC means that the film is composed of 19 pairs of layers deposited in 19 cycles, on the top of which a final layer of PLL 20 -FITC has been adsorbed. Each film observed at a given stage of the construction corresponds to a totally new buildup.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

“…The films containing PLL͞HA are expected to be highly hydrated because both polyelectrolytes are hydrophilic. For PLL, the amount of water uptaken can be as high as 50% (wt͞wt) (20) and HA hydrates by taking up as much as 1,000-fold its dry weight (21). Therefore, for such systems, it is crucial to study the film structure in situ to avoid artifacts introduced by drying, extensive fixation, or embedding protocols.…”

mentioning

confidence: 99%

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Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers

Picart

Mutterer

Richert

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

848

1,059

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The structure of poly(L-lysine) (PLL)͞hyaluronan (HA) polyelectrolyte multilayers formed by electrostatic self-assembly is studied by using confocal laser scanning microscopy, quartz crystal microbalance, and optical waveguide lightmode spectroscopy. These films exhibit an exponential growth regime where the thickness increases exponentially with the number of deposited layers, leading to micrometer thick films. Previously such a growth regime was suggested to result from an ''in'' and ''out'' diffusion of the PLL chains through the film during buildup, but direct evidence was lacking. The use of dye-conjugated polyelectrolytes now allows a direct three-dimensional visualization of the film construction by introducing fluorescent polyelectrolytes at different steps during the film buildup. We find that, as postulated, PLL diffuses throughout the film down into the substrate after each new PLL injection and out of the film after each PLL rinsing and further after each HA injection. As PLL reaches the outer layer of the film it interacts with the incoming HA, forming the new HA͞PLL layer. The thickness of this new layer is thus proportional to the amount of PLL that diffuses out of the film during the buildup step, which explains the exponential growth regime. HA layers are also visualized but no diffusion is observed, leading to a stratified film structure. We believe that such a diffusion-based buildup mechanism explains most of the exponential-like growth processes of polyelectrolyte multilayers reported in the literature.hyaluronan ͉ poly(L-lysine) ͉ confocal laser scanning microscopy ͉ diffusion ͉ film structure

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“…We also observed a (PLL͞HA) 20 multilayer in which the 14th and 20th HA (HA 14 -TR and HA 20 -TR) and only the last PLL (PLL 20 -FITC) layers were labeled (see Fig. 1C).…”

Section: Optical Waveguide Lightmode Spectroscopy (Owls)mentioning

confidence: 71%

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers

Picart

Mutterer

Richert

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

848

1,059

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“…For controls, EBs were differentiated on gelatinised coverslips in the presence of BMP 2 , TGFβ 1 , insulin and ascorbic acid (as a positive control; in this case, we added the growth factors after each change of the media; if not we had no bone induction) and fixed on day 21 with 2% paraforaldehyde. Immunocytochemistry was done as earlier described (31). Cells were first treated with a monoclonal mouse antiosteopontin antibody (OPN [Akm2A1]: sc-21742; Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

“…TGFβ 1 (25 kDa) influences the proliferation and differentiation of the stem cells (28), while BMP 2 (26 kDa) stimulates the production of specific bone matrix proteins (29,30). The proteins were embedded within a film of alternating layers of the polypeptides poly-L-lysine (PLL) and poly-L-glutamic acid (PGA) (31,32). Local degradation of PLL/PGA films by cells attached onto the surface of such films has been proposed as the mechanism by which cells gain access to biomolecules embedded within films.…”

mentioning

confidence: 99%

Active multilayered capsules for in vivo bone formation

Facca

Cortez

Mendoza-Palomares

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

118

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Interest in the development of new sources of transplantable materials for the treatment of injury or disease has led to the convergence of tissue engineering with stem cell technology. Bone and joint disorders are expected to benefit from this new technology because of the low self-regenerating capacity of bone matrix secreting cells. Herein, the differentiation of stem cells to bone cells using active multilayered capsules is presented. The capsules are composed of poly-L-glutamic acid and poly-L-lysine with active growth factors embedded into the multilayered film. The bone induction from these active capsules incubated with embryonic stem cells was demonstrated in vitro. Herein, we report the unique demonstration of a multilayered capsule-based delivery system for inducing bone formation in vivo. This strategy is an alternative approach for in vivo bone formation. Strategies using simple chemistry to control complex biological processes would be particularly powerful, as they make production of therapeutic materials simpler and more easily controlled.

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Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,174

1,184

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A bit of historyThe key role of surfaces for many material properties as well as for many biological processes has been recognized now. Therefore, new strategies aim at the tailoring of the material's surface only -or of a thin surface layer, respectively -, while preserving the bulk properties of the underlying support. Particular emphasis has been given to the surface modification by polymers, in an attempt to extend the known versatility of polymer bulk materials to ultrathin films and coatings, and to prepare bulk-surface composite materials. The self-organization of polymers has been increasingly explored for the preparation of well-defined surfaces and interfaces in recent years, extending the use of the established methods of low molar mass compounds (for comparative reviews, see ref. 1-3)). With such techniques, polymer films are formed spontaneously on substrates, due to balanced interactions between substrate, polymer (or its precursor) and medium. Typically, very thin, often monomolecular layers are produced. Repetitive deposition steps provide a precise control over the total thickness of the coatings, in the range from a few angstroms up to the micrometer range. Moreover, the step-by-step procedures allow for a fine structuring in the third dimension. In addition to the preparation of uniform and homogeneous coatings, gratings, gradients or steps of defined height in molecular dimensions can be easily constructed.The most recent of the self-organization techniques is the alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA) [3][4][5][6][7][8][9][10] . Although some early, singular studies of self-assembly by alternating adsorption of oppositely charged polyions are reported [11][12][13] , a practical method for ESA was developed Feature Article: The article presents the state-of-the-art of alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA), for the preparation of thin polymer coatings. In comparison to other, more established self-organization techniques, this recent method is distinguished by its simplicity, versatility, and speed. In particular, the tendency for self-healing is unique. Emphasis is given to the role of the molecular structure of the polyelectrolytes, and to the nature of the support. Also, various parameters for the preparation of multilayer films are highlighted, which are very important due to the kinetic control of the build-up process. The structure of the resulting coatings, their quality and stability, chemical reactions in the films, and potential applications are discussed.Macromol. Rapid Commun. 21, No. 7

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Ultrathin Polypeptide Multilayer Films for the Fabrication of Model Liquid/Liquid Electrochemical Interfaces

Cited by 77 publications

References 37 publications

Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers

Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers

Active multilayered capsules for in vivo bone formation

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

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