Umbelliferone Phosphate as a Substrate for Acid and Alkaline Phosphatase

Reduction and stereochemical studies through n.m.r. and X-ray techniques of indolo[2,1-b]quinazolines

“…A ring opening related to the elimination [(31)-+(34)] had earlier been reported by Fryer et af., 22 who found that compound (16b), on treatment with LiAlH, in THF, gave the amine (39). The reaction pathway was assumed to include compound (16c) as an intermediate.…”

Enzyme Activities in Human Liver Biopsies: Assay Methods and Activities of Some Lysosomal and Membrane-Bound Enzymes in Control Tissue and Serum

“…The blank values and the stability of the substrates stored in a desiccator were consistently satisfactory (Guilbault et al, 1968). In view of the extreme sensitivity of the assays it was important to use freshly prepared buffers as even minimal bacterial contamination could give elevated and erratic blank readings.…”

“…Guilbault, Sadar, Glazer & Haynes (1968) have compared several phosphate derivatives as substrates for alkaline phosphatase assays and found the Cmethylumbelliferyl derivative to be the most satisfactory. The pH optimum of 9-5 is lower than that usually recorded for alkaline phosphatase but is almost certainly due to the low substrate concentration employed in the present work (Motzok & Branion, 1959;Skillen & Harrison, 1973;Morton, 1957).…”

Phototautomerism of Warfarin Cation in Lowest Excited Singlet State via an Intramolecular Hydrogen Bridge

“…7-Hydroxycoumarin (umbelliferone) has been employed as a fluorescent indicator in fluorometric titrations and as a fluorogenic substrate in the study of the kinetics of hydrolytic reactions catalyzed by phosphatase and sulfatase (1)(2)(3)(4). 7-Hydroxycoumarin (umbelliferone) has been employed as a fluorescent indicator in fluorometric titrations and as a fluorogenic substrate in the study of the kinetics of hydrolytic reactions catalyzed by phosphatase and sulfatase (1)(2)(3)(4).…”