Unclear but present danger: An asymptomatic SARS-CoV-2 carrier

Ran, Dongchuan; Wang, Jinhui; Qin, Yuan; Liu, Ruishan; Xueli, Shi; Wang, Yiping; Xie, Chang Zhan; Jiang, Jia; Zhou, Jian

doi:10.1016/j.gendis.2020.07.010

Cited by 13 publications

(10 citation statements)

References 16 publications

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“…Yu and Yang ( 30 ) described an asymptomatic carrier who had transmitted SARS-CoV-2 to his brother following close contact. Recently, an asymptomatic carrier resulted in 13 secondary cases of infection, reportedly as an asymptomatic super spreader ( 34 ). In summary, it is almost unquestionable that asymptomatic carriers have infectivity.…”

Section: Infectivity and Transmission Risk Of Asymptomatic Carriermentioning

confidence: 99%

Clinical characteristics and infectivity of asymptomatic carriers of SARS‑CoV‑2 (Review)

Zhang

Zhou

et al. 2020

Exp Ther Med

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Since December 2019, the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. At present, confirmed patients are the main source of infection, while a number of studies have indicated that asymptomatic carriers also have the ability to spread the virus. As of September 29, 2020, as the first country to report coronavirus disease 2019 (COVID-19), China has 375 asymptomatic infections according to the National Health Commission of China. Asymptomatic carriers have become the current focus of global epidemic prevention and control efforts. The present review article provides a brief introduction on the clinical characteristics and infectivity of asymptomatic carriers, and makes suggestions for the identification of asymptomatic carriers. Contents

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Section: Infectivity and Transmission Risk Of Asymptomatic Carriermentioning

confidence: 99%

Clinical characteristics and infectivity of asymptomatic carriers of SARS‑CoV‑2 (Review)

Zhang

Zhou

et al. 2020

Exp Ther Med

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“…In severe cases, SARS-CoV-2 can cause pneumonia, acute respiratory distress syndrome, organ failure, and can even lead to death [ 4 ]. High proportion of infected persons are asymptomatic (40 to 45%) and can transmit the virus for up to 14 days, accounting for an important source of virus propagation since they can go undetected without an appropriate diagnosis [ 5 , 6 ]. One of the main strategies to monitor and control the viral dissemination has been the massive application of detection tests, which allow to identify and isolate infected individuals [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP

Tapia-Sidas¹,

Vargas-Hernández²,

Ramírez-Pool³

et al. 2023

PLoS ONE

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The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing to detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 diagnostic assays, are available to attempt to cover the global demand. Loop-mediated isothermal amplification (LAMP) based methods have been established as rapid, accurate, point of care diagnostic tests for viral infections; hence, they represent an excellent alternative for SARS-CoV-2 detection. The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase from Bacillus stearothermophilus and thermostable engineered reverse transcriptase from Moloney Murine Leukemia Virus were expressed using a prokaryotic system and purified by fast protein liquid chromatography. These enzymes were used to set up fluorometric real time and colorimetric end-point RT-LAMP assays. Several reaction conditions were optimized such as reaction temperature, Tris-HCl concentration, and pH of the diagnostic tests. The key enzymes for RT-LAMP were purified and their enzymatic activity was determined. Standardized reaction conditions for both RT-LAMP assays were 65°C and a Tris-HCl-free buffer at pH 8.8. Colorimetric end-point RT-LAMP assay was successfully used for viral detection from clinical saliva samples with 100% sensitivity and 100% specificity compared to the results obtained by RT-qPCR based diagnostic protocols with Ct values until 30. The developed RT-LAMP diagnostic tests based on purified recombinant enzymes allowed a sensitive and specific detection of the nucleocapsid gene of SARS-CoV-2.

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“…However, most of the questionnaires used have focused on certain demographics or particular infection control practices. Also, most of the studies that have surveyed healthcare workers did not utilize laboratory testing to confirm the status of the controls [ 15 ], despite the fact that asymptomatic SARS-CoV-2 infection may play a role in super-spreader transmission events [ 16 , 17 ]. The aim of this study is to better understand and evaluate the association between several infection control factors and the risk of SARS-CoV-2 infection and potentially assess effectiveness of infection control measures.…”

Section: Introductionmentioning

confidence: 99%

Factors associated with SARS-CoV-2 infection amongst healthcare workers in a COVID-19 designated hospital

Youha

Alowaish

Ibrahim

et al. 2021

Journal of Infection and Public Health

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Background Understanding the risk factors responsible for the increased infection among HCWs can mitigate the transmission of COVID-19 among HCWs and patients alike. The aim of this study is to evaluate factors associated with SARS-CoV-2 infection among healthcare Workers. Methods Healthcare workers and hospital administrators were asked to participate in this cross-sectional survey study that was conducted in Jaber Al Ahmad Hospital (JAH) between August to October 2020. Participants were invited to undergo SARS-CoV-2-specific antibody testing and to complete a questionnaire targeted to factors that may be associated with acquisition of SARS-CoV-2. Descriptive analysis and multivariate logistic regression were done. Results 847 healthcare workers participated in the study and 20.5 % of them had previous SARS-CoV-2 infection. The average age of participants was 35.7 years (SD = 7.9); 52.4% were female, and 55.8% were doctors. Multivariate analysis showed that working as a nurse (adjusted OR 1.77, 95% CI = 1.15, 2.71), and wearing gloves (adjusted OR 2.93, 95% CI = 1.19, 7.22) were significantly associated with an increased likelihood of contracting SARS-CoV-2 infection while controlling for other factors. Most personal protective equipment (PPE) were reported to be available always or most of the time, with the least available PPE item being coveralls (74.4%). Conclusions After adjusting for confounding factors, being a nurse and prolonged glove use were associated with increased likelihood of SARS-CoV-2 infection. Prospective cohort studies are required to further elucidate the reasons for our findings in order to minimize the transmission of infection among healthcare workers.

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Unclear but present danger: An asymptomatic SARS-CoV-2 carrier

Cited by 13 publications

References 16 publications

Clinical characteristics and infectivity of asymptomatic carriers of SARS‑CoV‑2 (Review)

Clinical characteristics and infectivity of asymptomatic carriers of SARS‑CoV‑2 (Review)

Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP

Factors associated with SARS-CoV-2 infection amongst healthcare workers in a COVID-19 designated hospital

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