Unconventional TLC systems in lipophilicity determination: A review

Wicha-Komsta, Katarzyna; Komsta, Łukasz

doi:10.1080/10826076.2017.1298023

Cited by 10 publications

(6 citation statements)

References 73 publications

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“…The main reason for this fact can be, for example, the different adsorbing properties of applied stationary phases or eluents such as their elution strength or surface tension, which show an influence on the results of the R M parameter. As was confirmed in previous studies, the pH value of distilled water used as a mobile phase component also has a large impact on the value of both the chromatographic lipophilicity parameters and the experimental partition coefficients [8,11,18,31].…”

Section: Resultssupporting

confidence: 55%

“…In addition to experimental methods, in order to determine the n -octanol-water partition coefficient (logP), different theoretical methods (computer programs) allowing the initial assessment of the logP value can also be applied [3]. Many various lipophilicity descriptors and methods have been well described and proposed for the evaluation of the lipophilic properties of different compounds in review articles from recent years [7,8,9]. Currently, the chromatographic approach is used the most [10,11,12,13,14,15,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Utility of RP-TLC Technique and Different Computational Methods to Assess the Lipophilicity of Selected Antiparasitic, Antihypertensive, and Anti-inflammatory Drugs

2019

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The aim of this study was to assess the lipophilicity of selected antiparasitic, antihypertensive and non-steroidal anti-inflammatory drugs (NSAIDs) by means of reversed phase–thin layer chromatography (RP-TLC) as well by using Soczewiński–Wachtmeister’s and J. Ościk’s equations. The lipophilicity parameters of all examined compounds obtained under various chromatographic systems (i.e., methanol-water and acetone-water, respectively) and those determined on the basis of Soczewiński-Wachtmeister’s and Ościk’s equations (i.e., RMWS and RMWO) were compared with the theoretical ones (e.g., AlogPs, AClogP, milogP, AlogP, MlogP, XlogP2, XlogP3) and the experimental value of the partition coefficient (logPexp). It was found that the RMWS parameter may be a good alternative tool in describing the lipophilic nature of biologically active compounds with a high and low lipophilicity (i.e., antihypertensive and antiparasitic drugs). Meanwhile, the RMWO was more suitable for compounds with a medium lipophilicity (i.e., non-steroidal anti-inflammatory drugs). The chromatographic parameter 0(a) can be helpful for the prediction of partition coefficients, i.e., AClogP, XlogP3, as well as logPexp of examined compounds.

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Section: Resultssupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Comparison of the Utility of RP-TLC Technique and Different Computational Methods to Assess the Lipophilicity of Selected Antiparasitic, Antihypertensive, and Anti-inflammatory Drugs

2019

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“…In chromatographic methods, confirmation of identity is obtained by comparing the retention times or retardation factors (TLC) of the test substance and the reference substance [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug, impurities present in the drug, but also for investigating many physicochemical properties, including lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

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An easy and short-time TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyzes were performed on chromatographic plates precoated with silica gel 60F254 using the optimal mobile phase: chloroform + methanol + diethylamine in a volume ratio of 9:1:1. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopoeial method. The presented method is fast, sensitive, precise, selective, accurate and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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“…The most commonly used methods for examining the identity of drugs are infrared absorption spectrophotometry, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas chromatography (GC) [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug and any impurities present in the drug, as well as for investigating many physicochemical properties, including the lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

Pyka-Pająk

2024

Processes

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A sensitive, easy-to-use, fast, and cost-effective TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyses were performed on chromatographic plates precoated with silica gel 60F254 using chloroform + methanol + diethylamine in a volume ratio of 9:1:1 as the optimal mobile phase. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopeial method. The presented method is fast, sensitive, precise, selective, accurate, and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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Unconventional TLC systems in lipophilicity determination: A review

Cited by 10 publications

References 73 publications

Comparison of the Utility of RP-TLC Technique and Different Computational Methods to Assess the Lipophilicity of Selected Antiparasitic, Antihypertensive, and Anti-inflammatory Drugs

Comparison of the Utility of RP-TLC Technique and Different Computational Methods to Assess the Lipophilicity of Selected Antiparasitic, Antihypertensive, and Anti-inflammatory Drugs

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

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