Under the dynamic environment of normal diagnosis and treatment in the bronchoscope room, the study of the removal efficiency of the new air purifier on microbial aerosols

Abstract

Background The SARS-CoV-2 can spread through droplets, aerosols, etc. In the case of a severe outbreak of the SARS-CoV-2, the use of new air purifiers in work and living places has a certain effect on reducing the spread of the SARS-CoV-2. There are abundant microbial aerosols in the indoor medical environment. The innovation of this experiment lies in the use of long-term, large-volume sampling methods under the multi-factor dynamic conditions of normal diagnosis and treatment in the bronchoscope room, and the use of two microbial detection methods at the same time to study the removal efficiency of the new air purifier on microbial aerosols ; The innovation of the new air purifier lies in outdoor fresh air and steady-state displacement flow technology. Methods In this experiment, the test group and the control group were set up. Gelatin filtration membrane and PTFE filtration membrane were used to sample the microbial aerosols in the bronchoscope room. Long time (39.5h) and large sampling volume (70590L) was used. A total of 16 days of pure working time sampling was carried out for a period of 1 month. The collected specimens were tested by two methods: next-generation sequencing (mNGS) and microbial culture identification. The researcher retrieved and recorded the microbiological test results of related patients, and compared them with the results of this experiment. Results The result of next-generation sequencing (mNGS): The total purification efficiency was 88.0%. Microbial culture counting and identification results: the total purification efficiency is 87.5%. The results are statistically significant. There is a certain correlation between the experimental results and the clinical microbiological test results of patients. Conclusions 1. The new type of air purifier has a good effect on the removal of microbial aerosols in the bronchoscope room; 2. A variety of microorganisms involved in the experiment can be transmitted through aerosols. Discussion The new air purifier has a total removal efficiency of more than 85% of microorganisms under the dynamic environment of the hospital's normal diagnosis and treatment, and the purification efficiency of a single microorganism ranges 50%-100%. Such removal efficiency may have a positive effect for the control of the SARS-CoV-2 epidemic.