Underlying molecular and cellular mechanisms in childhood irritable bowel syndrome

Chumpitazi, Bruno P.; Shulman, Robert J.

doi:10.1186/s40348-016-0036-8

Cited by 47 publications

(47 citation statements)

References 91 publications

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“…The time of weaning is an important development time and our findings may have implications in understanding the impact of early stress on the onset of IBS in children. 51 Moreover, alterations induced by LNS may have relevance to the long-lasting consequences of early adverse life events reported in IBS, alterations of HPA axis and its prevalence in women. 2,52 Acknowledgements: We acknowledge Dr Patrick Frost (Department of Hematology-Oncology, UCLA) for the plate reader use.…”

Section: Discussionmentioning

confidence: 99%

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Moussaoui

Jacobs

Larauche

et al. 2017

J Neurogastroenterol Motil

111

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Background/AimsWistar rat dams exposed to limited nesting stress (LNS) from post-natal days (PND) 2 to 10 display erratic maternal behavior, and their pups show delayed maturation of the hypothalamic-pituitary-adrenal axis and impaired epithelial barrier at PND10 and a visceral hypersensitivity at adulthood. Little is known about the impact of early life stress on the offspring before adulthood and the influence of sex. We investigated whether male and female rats previously exposed to LNS displays at weaning altered corticosterone, intestinal permeability, and microbiota. MethodsWistar rat dams and litters were maintained from PND2 to 10 with limited nesting/bedding materials and thereafter reverted to normal housing up to weaning (PND21). Control litters had normal housing. At weaning, we monitored body weight, corticosterone plasma levels (enzyme immunoassay), in vivo intestinal to colon permeability (fluorescein isothiocyanate-dextran 4 kDa) and fecal microbiota (DNA extraction and amplification of the V4 region of the 16S ribosomal RNA gene). ResultsAt weaning, LNS pups had hypercorticosteronemia and enhanced intestinal permeability with females > males while body weights were similar. LNS decreased fecal microbial diversity and induced a distinct composition characterized by increased abundance of Gram positive cocci and reduction of fiber-degrading, butyrate-producing, and mucus-resident microbes. ConclusionsThese data indicate that chronic exposure to LNS during the first week post-natally has sustained effects monitored at weaning including hypercorticosteronemia, a leaky gut, and dysbiosis. These alterations may impact on the susceptibility to develop visceral hypersensitivity in adult rats and have relevance to the development of irritable bowel syndrome in childhood.

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Section: Discussionmentioning

confidence: 99%

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Moussaoui

Jacobs

Larauche

et al. 2017

J Neurogastroenterol Motil

111

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“…1 Clinically IBS is characterized by abdominal discomfort that improves with defecation and/or is associated with changes in stool frequency or stool form.…”

Section: Introductionmentioning

confidence: 99%

Stooling Characteristics in Children With Irritable Bowel Syndrome

Weidler

Self

Czyzewski

et al. 2017

Clinical Gastroenterology and Hepatology

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“…Mast cells are also a source of serotonin and the number of mast cells is increased in adults with irritable bowel syndrome (IBS) [22]. Similar results were obtained by Cremon et al who demonstrated a 10-fold higher serotonin release in patients with IBS [24].…”

Section: Discussionmentioning

confidence: 72%

Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts

et al. 2016

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In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.

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Underlying molecular and cellular mechanisms in childhood irritable bowel syndrome

Cited by 47 publications

References 91 publications

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Chronic Early-life Stress in Rat Pups Alters Basal Corticosterone, Intestinal Permeability, and Fecal Microbiota at Weaning: Influence of Sex

Stooling Characteristics in Children With Irritable Bowel Syndrome

Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts

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