Understanding and controlling the bone–implant interface

Da, Puleo; Nanci, Antonio

doi:10.1016/s0142-9612(99)00160-x

Cited by 1,192 publications

(887 citation statements)

References 76 publications

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“…Numerous reports have demonstrated that implants with micron-scale topography enable rapid and increased bone accrual or bone implant contact [21][22][23][24][25][26][27]. There are several processes that can be used to achieve implant surfaces with micro-scale topography.…”

Section: Discussionmentioning

confidence: 99%

“…For example, using fluorochrome labeling D.A. Puleo indicated that bone grew in two directions, and bone extending away from the implant formed at a rate about 30% faster than that of the bone moving toward the implant [24]. P. Thomsen et al observed two types of bone, calcified bone tissue and dense amorphous bone, near implant surface [28].…”

Section: Discussionmentioning

confidence: 99%

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Microscopic view of osseointegration and functional mechanisms of implant surfaces

Han

Shen

2015

Materials Science and Engineering: C

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Argon ion beam polishing technique was applied to prepare the cross sections of implants feasible for high resolution scanning electron microscope investigation. The interfacial microstructure between newly formed bone and implants with three modified surfaces retrieved after in vivo test using three different animal models was characterized. By this approach it has become possible to directly observe early bone formation, the increase of bone density, and the evolution of bone structure. The two bone growth mechanisms, distant osteogenesis and contact osteogenesis, can also be distinguished. These direct observations give, at microscopic level, a better view of osseointegration and expound the functional mechanisms of various implant surfaces for osseointegration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microscopic view of osseointegration and functional mechanisms of implant surfaces

Han

Shen

2015

Materials Science and Engineering: C

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“…1,12-15 In addition, both of these proteins have been found to be enriched at bone-implant interfaces and they are hypothesized to play an important role in cellular adhesion and osseointegration at this interface as well. 16 OPN and BSP are both members of the SIBLING (small integrin-binding ligand, N-linked glycoproteins) family, one category of non-collagenous proteins found in many mineralized tissues. The SIBLING family members share a number of properties, including that they are secreted, phosphorylated, and sulfated sialoproteins, that are acidic in nature.…”

Section: Introductionmentioning

confidence: 99%

MC3T3-E1 cell adhesion to hydroxyapatite with adsorbed bone sialoprotein, bone osteopontin, and bovine serum albumin

Bernards

Qin

Jiang

2008

Colloids and Surfaces B: Biointerfaces

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Native bone tissue is composed of a complex matrix of collagen, non-collagenous proteins, and hydroxyapatite (HAP). Bone sialoprotein (BSP) and bone osteopontin (OPN) are members of the non-collagenous protein family termed the SIBLING (small integrin-binding ligand, N-linked glycoproteins) proteins, which are primarily found in mineralized tissues. Previously, OPN was shown to exhibit a preferential orientation for MC3T3-E1 cell adhesion when it was specifically bound to collagen, while the MC3T3-E1 cell adhesion was shown to be dependant on the conformational flexibility of BSP specifically bound to collagen. Additionally, OPN was shown to play a greater role than BSP for cell binding to collagen. In this work, the orientations and conformations of BSP and OPN specifically bound to HAP are probed under similar conditions. Radiolabeled adsorption isotherms were obtained for BSP and OPN on HAP formed from a simulated body fluid, and the results show that HAP has the capacity to bind significantly more BSP than OPN. An in vitro MC3T3-E1 cell adhesion assay was then performed to compare the cell binding ability of adsorbed BSP and OPN specifically bound to HAP. It was found that there is a preference for cell binding to HAP with adsorbed BSP as compared to OPN, but not to a statistically significant level. However, the maximum cell binding was observed on HAP substrates with adsorbed heat denatured bovine serum albumin (BSA). The influence of BSA on cell binding was shown to be concentration dependant and it is believed that the adsorbed BSA modulates the proliferation state of the bound cells.

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“…[4][5][6] On the other hand, phospholipids are widely used to modify medical devices, including biosensors, drug carriers, and urological devices in order to achieve more desirable biological integrations. 7 The adsorption of proteins to lipid layers is recognized as the first event following the implantation of biomaterials and has been shown to play an important role in determining subsequent events such as thrombus formation, foreign body reaction, and other undesirable responses. 8,9 The function of proteins is realized by their residues, whose capability is greatly affected by their local environment.…”

Section: Introductionmentioning

confidence: 99%

Penetration and Saturation of Lysozyme in Phospholipid Bilayers

et al. 2007

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We show that the combination of X-ray reflectivity, tryptophan fluorescence spectrum, and fluorescence quenching by bromine provides a useful tool to probe the location of lysozyme in lipid bilayers. To this end, we prepare lamellar complexes composed of phospholipids and lysozyme on solid surfaces using a solutioncasting method. The proteins lie spontaneously between adjacent bilayers in the complexes. The results indicate that lysozyme may penetrate into the lipid bilayers. But the penetration depth is very shallow, and the tryptophan residues do not penetrate beyond the interface between the hydrocardon core and the headgroup region of the lipid bilayer. The penetration becomes saturated when more proteins are incorporated into the lamellar complex. The excess proteins stay in the interlamellar aqueous layers.

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Understanding and controlling the bone–implant interface

Cited by 1,192 publications

References 76 publications

Microscopic view of osseointegration and functional mechanisms of implant surfaces

Microscopic view of osseointegration and functional mechanisms of implant surfaces

MC3T3-E1 cell adhesion to hydroxyapatite with adsorbed bone sialoprotein, bone osteopontin, and bovine serum albumin

Penetration and Saturation of Lysozyme in Phospholipid Bilayers

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