Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Chow, Yiing Yng; Rahman, Sadequr; Ting, Adeline Su Yien

doi:10.1016/j.jare.2016.10.008

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…In the current study, the isolated endophytes showed a significant antagonistic effect against different pathogenic microorganisms. Endophytic bacteria can indirectly assist plant growth through the production of substances, which inhibit plant pathogens [52,53]. Endophytes isolated from other medicinal plants have also produced novel bioactive compounds [49,54].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

et al. 2020

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Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (Fagonia mollis Delile and Achillea fragrantissima (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (Zea mays L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL−1 with increasing tryptophan concentration from 1 to 5 mg·mL−1. Bacillus and Brevibacillus strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

et al. 2020

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“…For biotechnological application of endophytes as biocontrol, the selection of microbial inoculants in regard to their direct ability to promote plant growth is crucial. However, other mechanisms which enable microbial inoculants to establish mutualism with plants without severe any detrimental symptoms are necessary required [50] .…”

Section: Discussionmentioning

confidence: 99%

Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L.

Hassan

2017

Journal of Advanced Research

263

109

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“…and Trichoderma spp. ( Bakhtiar et al., 2010 ; Sundram et al., 2011 ; Azizah et al., 2015 ; Goh et al., 2015 ; Chow et al., 2017 ; Lim et al., 2018 ; Chow et al., 2019 ; Sujarit et al., 2020 ). Inhibition happened attributed to some potential antimicrobials compound such as eudistomin I, Gly-Met-OH, halstoctacosanolide A, lovastatin, naringenin, N-acetyl-leu-leu-tyr-amide, N-methyl-a-aminoisobutyric acid, penipanoid A, phenazine and phenazine-1-carboxylic acid, phenylethyl alcohol, phenyl alkenoic acids, pyrene-1,6-dione, 4-O-8’,5”-5’-dehydrotriferulic acid, 7-O-beta-D-glucoside, 12-deoxyaklanonic acid, and 12-oxo-10Z-dodecenoic acid ( Angel et al., 2016 ; Alexander et al., 2017b ; Lee et al., 2018 ; Ahmad et al., 2020 ; Sujarit et al., 2020 ).…”

Section: Management Strategiesmentioning

confidence: 99%

Ganoderma boninense: general characteristics of pathogenicity and methods of control

Khoo

Chong

2023

Front. Plant Sci.

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Ganoderma boninense (G. boninense) is a soil-borne fungus threatening oil palm at the present. It causes basal stem rot disease on oil palm. Within six months, this fungus can cause an oil palm plantation to suffer a significant 43% economic loss. The high persistence and nature of spread of G. boninense in soil make control of the disease challenging. Therefore, controlling the pathogen requires a thorough understanding of the mechanisms that underlie pathogenicity as well as its interactions with host plants. In this paper, we present the general characteristics, the pathogenic mechanisms, and the host’s defensive system of G. boninense. We also review upcoming and most promising techniques for disease management that will have the least negative effects on the environment and natural resources.

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Understanding colonization and proliferation potential of endophytes and pathogen in planta via plating, polymerase chain reaction, and ergosterol assay

Abstract: Graphical abstractColonization and proliferation potential of endophytes and pathogen in planta via ergosterol assay and compared to conventional plating and PCR methods.

Cited by 14 publications

References 37 publications

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

Plant growth-promoting activities for bacterial and fungal endophytes isolated from medicinal plant of Teucrium polium L.

Ganoderma boninense: general characteristics of pathogenicity and methods of control

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