Understanding protein-nanoparticle interactions leading to protein corona formation: In vitro - in vivo correlation study

Marques, Cintia; Maroni, Plinio; Maurizi, Lionel; Jordan, Olivier; Borchard, Gerrit

doi:10.1016/j.ijbiomac.2023.128339

Cited by 9 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This correlation aligns with their findings on in vivo cellular interactions investigated across different species environments. In Marques et al's study, 65 the in vitro single protein-NP studies were comparable to in vivo protein corona data, demonstrating the successful translation of direct results from in vitro studies into in vivo investigations. In our prior research, we aimed to understand HC-mediated cellular behavior using a well-established model of mouse-derived macrophages (J774.A1).…”

Section: Resultsmentioning

confidence: 83%

Graphene Oxide Nanosheets Toxicity in Mice Is Dependent on Protein Corona Composition and Host Immunity

Li,

Mei,

Liam-Or

et al. 2024

ACS Nano

View full text Add to dashboard Cite

Two-dimension graphene oxide (GO) nanosheets with high and low serum protein binding profiles (high/low hard-bound protein corona/HC high/low ) are used in this study as model materials and screening tools to investigate the underlying roles of the protein corona on nanomaterial toxicities in vivo. We proposed that the in vivo biocompatibility/nanotoxicity of GO is protein corona-dependent and host immunity-dependent. The hypothesis was tested by injecting HC high/low GO nanosheets in immunocompetent ICR/CD1 and immunodeficient NOD-scid II2rγ null mice and performed histopathological and hematological evaluation studies on days 1 and 14 postinjection. HC low GO induced more severe acute lung injury compared to HC high GO in both immunocompetent and immunodeficient mice, with the effect being particularly pronounced in immunocompetent animals. Additionally, HC low GO caused more significant liver injury in both types of mice, with immunodeficient mice being more susceptible to its hepatotoxic effects. Moreover, administration of HC low GO resulted in increased hematological toxicity and elevated levels of serum pro-inflammatory cytokines in immunocompromised and immunocompetent mice, respectively. Correlation studies were conducted to explore the impact of distinct protein corona compositions on resulting toxicities in both immunocompetent and immunodeficient mice. This facilitated the identification of consistent patterns, aligning with those observed in vitro, thus indicating a robust in vitro−in vivo correlation. This research will advance our comprehension of how hard corona proteins interact with immune cells, leading to toxicity, and will facilitate the development of improved immune-modulating nanomaterials for therapeutic purposes.

show abstract

Section: Resultsmentioning

confidence: 83%

Graphene Oxide Nanosheets Toxicity in Mice Is Dependent on Protein Corona Composition and Host Immunity

Li,

Mei,

Liam-Or

et al. 2024

ACS Nano

View full text Add to dashboard Cite

show abstract

“…Work using gold nanoparticles revealed a 4.5-fold higher binding affinity of hemoglobin to small nanoparticles (40 nm) as compared to larger ones (70 nm), [33] which may partially explain adsorption to the smaller PLGA-PEG particles (72 nm). Further, hemoglobin readily adsorbs to negatively charged nanoparticles, [34][35] which may further increase its affinity for the negatively charged PLGA-PEG particles (-34 mV).…”

Section: The Composition Of Csf-and Serum-derived Protein Coronas Are...mentioning

confidence: 99%

Protein Coronas Derived from Cerebrospinal Fluid Enhance the Interactions Between Nanoparticles and Brain Cells

Morshed,

Rennie,

Faria

et al. 2024

Preprint

View full text Add to dashboard Cite

Neuronanomedicine harnesses nanoparticle technology for the treatment of neurological disorders. An unavoidable consequence of nanoparticle delivery to biological systems is the formation of a protein corona on the nanoparticle surface. Despite the well-established influence of the protein corona on nanoparticle behavior and fate, as well as FDA approval of neuro-targeted nanotherapeutics, the effect of a physiologically relevant protein corona on nanoparticle-brain cell interactions is insufficiently explored. Indeed, less than 1% of protein corona studies have investigated protein coronas formed in cerebrospinal fluid (CSF), the fluid surrounding the brain. Herein, we utilize two clinically relevant polymeric nanoparticles (PLGA and PLGA-PEG) to evaluate the formation of serum and CSF protein coronas. LC-MS analysis revealed distinct protein compositions, with selective enrichment/depletion profiles. Following incubation with brain cells, serum and CSF coronas on PLGA particles showed enhanced associations with all cell types as compared to their corresponding corona on PLGA-PEG particles. CSF-derived protein coronas on PLGA nanoparticles, specifically, showed the greatest nanoparticle-cell interactions, with Pearson’s correlation analysis revealing that proteins associated with enhanced nanoparticle-cell interactions were exclusively enriched in this protein corona. This study demonstrates the importance of correct choice of physiologically relevant biological fluids, and its influence on the formation of the protein corona, subsequent nanoparticle-cell interactions.

show abstract

Proline-Functionalized ZnO (Zinc Oxide) Nano-Osmolytes: Potent Catalysts for Protein Aggregation Versus Pristine ZnO

Sharma,

Bhuyan,

Natu

et al. 2024

BioNanoSci.

View full text Add to dashboard Cite

Understanding protein-nanoparticle interactions leading to protein corona formation: In vitro - in vivo correlation study

Cited by 9 publications

References 54 publications

Graphene Oxide Nanosheets Toxicity in Mice Is Dependent on Protein Corona Composition and Host Immunity

Graphene Oxide Nanosheets Toxicity in Mice Is Dependent on Protein Corona Composition and Host Immunity

Protein Coronas Derived from Cerebrospinal Fluid Enhance the Interactions Between Nanoparticles and Brain Cells

Proline-Functionalized ZnO (Zinc Oxide) Nano-Osmolytes: Potent Catalysts for Protein Aggregation Versus Pristine ZnO

Contact Info

Product

Resources

About