Understanding Sperm Quality for Improved Reproductive Performance

Santolaria, Pilar; Rickard, Jessica P.; Pérez-Pe, Rosaura

doi:10.3390/biology12070980

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Sperm preservation or storage is a crucial reproductive technique for human fertility treatments, artificial livestock breeding, and animal conservation [ 1 ]. These procedures involve buffer systems and cryoprotective agents [ 2 ] and have been widely developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

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Background and Aim: Semen storage is an important reproductive method used in artificial livestock breeding. However, oxidative stress during storage reduces the quality of sperm. Melatonin supplementation in semen storage medium has not been well studied, but it has been shown to protect cells from oxidative stress. Therefore, this study aimed to determine the effect of melatonin supplementation on sperm quality parameters and antioxidant gene expression levels in semen extenders during cold storage. Materials and Methods: Semen extenders with melatonin concentrations of 0 (control), 0.1, 0.2, and 0.3 mM were added as treatment. The treated semen was then stored at 5°C for 72 h using a cold storage method, and quality parameters, including percentage of progressive motility, membrane integrity, intact acrosome, and DNA integrity, were measured every 24 h. In addition, messenger ribonucleic acid abundance levels of glutathione peroxidase (GPx) and superoxide dismutase (SOD) genes were sampled after 0 and 72 h of cold storage. Results: All observed sperm quality parameters decreased with increasing cold storage time; however, 0.2 mM melatonin demonstrated superior protection of sperm quality during cold storage. Gene expression analysis showed that GPx levels decreased significantly (p < 0.05) after 72 h in semen without melatonin but not in the melatonin-treated groups. A similar trend was also observed in SOD, indicating that exogenous antioxidants effectively protected the sperms. Conclusion: Melatonin supplementation at 0.2 mM in semen extenders during cold storage maintains sperm quality parameters for up to 72 h because melatonin protects sperm from oxidative stress. These findings can be used to improve the semen storage protocol by combining semen extender and antioxidant. Keywords: antioxidant gene expression, melatonin, semen cold storage, sperm quality.

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Section: Introductionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

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Fish Consumption Once a Week Improves Sperm Quality and Testosterone Levels

Roussev,

Sokrateva,

Nenkova

et al. 2024

Andrologia

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Background: The rate of male infertility has increased in the last decades. One factor that may improve sperm quality and testosterone is fish consumption, which supplies the organism with essential nutrients.Aim: To determine evidence of a relationship between the frequency of fish consumption and sperm quality.Methods: Seventy‐four healthy men, aged 25–45 years, were involved in this observational study. Semen quality was evaluated by spermogram analyses and testosterone was measured in blood. Volunteers were classified as low, moderate, and high fish consumption groups according to the replies indicated in a questionnaire they had to fill out.Results: The moderate fish consumption group showed better semen quality with all spermogram parameters in the reference range and the low fish consumption group manifested the worst semen quality with sperm morphology below the reference range. The low fish consumption group had lower testosterone levels (3.6 ± 0.9 vs. 4.3 ± 1.5; p = 0.04), decreased nonprogressive spermatozoa (11.9 ± 6.2 vs. 15.8 ± 8.2; p = 0.04), and lower semen volume (3.1 ± 2.0 vs. 3.6 ± 1.3; p = 0.06) compared to the moderate fish consumption group. Positive associations were detected between the frequency of fish consumption, the semen volume, and nonprogressive spermatozoa, respectively.Conclusions: Fish consumption at moderate level such as once a week leads to improvement in sperm quality and testosterone levels.

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Understanding Sperm Quality for Improved Reproductive Performance

Abstract: The assessment of semen quality is used to identify factors that influence sperm performance and diagnose male infertility [...]

Cited by 2 publications

References 11 publications

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Fish Consumption Once a Week Improves Sperm Quality and Testosterone Levels

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