Understanding the formation of CuS concave superstructures with peroxidase-like activity

Abstract

Copper sulfide (CuS) concave polyhedral superstructures (CPSs) have been successfully prepared in an ethanolic solution by a simple solvothermal reaction without the use of surfactants or templates. Two typical well defined, high symmetry CuS concave polyhedrons, forming a concave truncated cuboctahedron and icosahedron were prepared. The effect of the reaction time, temperature and different Cu ion and sulfur sources on the formation of CuS CPSs were investigated and a possible formation mechanism was proposed and discussed based on gas chromatography-mass spectrometry. More importantly, we found, for the first time, that the CuS CPSs exhibit intrinsic peroxidase-like activity, as they can quickly catalyze the oxidation of typical horseradish peroxidase (HRP) substrates, 3,3',5,5'-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD), in the presence of hydrogen peroxide. In addition to the recent discoveries regarding peroxidase mimetics on Fe(3)O(4) NPs and carbon nanostructures, our findings suggest a new kind of candidate for peroxidase mimics. This may open up a new application field of CuS micro-nano structures in biodetection, biocatalysis and environmental monitoring.

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

“…Furthermore, NiS/MMT/GO also possesses a higher thermal stability than that of HRP over a wide range of temperature from 20 to 80°C (Fig. 7b), because natural enzymes are vulnerable to denaturalization [34]. Generally, the results proved the excellent robustness of peroxidase activity of NiS/MMT/GO nanocomposites.…”

“…As expected, similar to natural enzyme, known as horseradish peroxidase (HRP), and other artificial enzyme mimics, the catalytic activity of NiS/MMT/GO is greatly dependent on pH and temperature [34]. The peroxidaselike activity of NiS/MMT/GO was investigated while varying the pH of reaction systems from 2 to 8 as well as the temperature in the range of 30 to 70°C, shown in Fig.…”

“…4b. Thus, NiS/MMT/GO displayed high catalytic activity over a broad range of temperatures, superior to that of the natural enzymes [34]. Conveniently, the following assays were conducted at room temperature unless otherwise stated.…”

AuPt Alloy Nanostructures with Tunable Composition and Enzyme-like Activities for Colorimetric Detection of Bisulfide

“…Compared with natural enzymes, nanostructured artificial enzymes have several advantages, such as easy synthesis, facile storage and high catalytic stability against stringent conditions. Until now, a variety of nanomaterials, including metal oxides345, metal sulfides67, noble metals891011, carbon1213, and their combined nanostructures1415 have been explored to exhibit enzyme-like activities. Because of the quantum size and surface effect, nanoparticles generally exhibit superior catalytic activity and intrinsic ability to generate or scavenge reactive oxygen species161718.…”

Nanotechnological Methods of Antioxidant Characterization

“…Nanoparticles of noble metals have been used to fabricate biosensors for the detection of hydrogen peroxide (e.g., in conjunction with glucose sensing) by making use of the oxidase-and peroxidase-like activity of NPs (74)(75)(76)(77)(78)(79)(80)(81). For example, AuNPs with different coatings and bovine serum albumin (BSA)-stabilized Au clusters exhibited peroxidase-like activity along with good biocompatibility, which may make AuNPs promising candidates for use in biosensors (82)(83)(84).…”