Understanding the interplay of membrane trafficking, cell surface mechanics, and stem cell differentiation

Li, Jia Hui; Trivedi, Vikas; Diz-Muñoz, Alba

doi:10.1016/j.semcdb.2022.05.010

Cited by 4 publications

(1 citation statement)

References 214 publications

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“…Our identification of Piezo1 as an initiator of EGFR endocytosis contributes to a growing list of membrane-based processes controlled by this channel that includes endosomal trafficking to the midbody during mitotic abscission ( 8 ) and phagocytosis ( 79 ). Membrane tension is both the fundamental activator of Piezo channels ( 80 , 81 ) and a key determinant of membrane remodeling and trafficking controlling pluripotency, differentiation, homeostatic turnover, and aging ( 82 – 84 ). Our work adds EGFR to the picture, highlighting the relevance of kinase-independent EGFR-driven mechanisms previously disregarded by a focus on EGFR mutations.…”

Section: Discussionmentioning

confidence: 99%

Piezo1 activates noncanonical EGFR endocytosis and signaling

Pardo-Pastor,

Rosenblatt

2023

Sci. Adv.

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EGFR-ERK signaling controls cell cycle progression during development, homeostasis, and disease. While EGF ligand and mechanical inputs can activate EGFR-ERK signaling, the molecules linking mechanical force to this axis have remained mysterious. We previously found that stretch promotes mitosis via the stretch-activated ion channel Piezo1 and ERK signaling. Here, we show that Piezo1 provides the missing link between mechanical signals and EGFR-ERK activation. While both EGF- and Piezo1-dependent activation trigger clathrin-mediated EGFR endocytosis and ERK activation, EGF relies on canonical tyrosine autophosphorylation, whereas Piezo1 involves Src-p38 kinase-dependent serine phosphorylation. In addition, unlike EGF, ex vivo lung slices treated with Piezo1 agonist promoted cell cycle re-entry via nuclear ERK, AP-1 (FOS and JUN), and YAP accumulation, typical of regenerative and malignant signaling. Our results suggest that mechanical activation via Piezo1, Src, and p38 may be more relevant to controlling repair, regeneration, and cancer growth than tyrosine kinase signaling via canonical EGF signaling, suggesting an alternative therapeutic approach.

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Section: Discussionmentioning

confidence: 99%

Piezo1 activates noncanonical EGFR endocytosis and signaling

Pardo-Pastor,

Rosenblatt

2023

Sci. Adv.

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Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling

He,

Tan,

et al. 2024

Adv Materials Technologies

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Regenerative therapies, including the transplantation of spinal cord progenitor cells (SCPCs) derived from induced pluripotent stem cells (iPSCs), are promising treatment strategies for spinal cord injuries. However, the risk of tumorigenicity from residual iPSCs advocates an unmet need for rapid SCPCs safety profiling. Herein, a rapid (≈3000 cells min‐1) electrical‐based microfluidic biophysical cytometer is reported to detect low‐abundance iPSCs from SCPCs at single‐cell resolution. Based on multifrequency impedance measurements (0.3 to 12 MHz), biophysical features including cell size, deformability, membrane, and nucleus dielectric properties are simultaneously quantified as a cell is hydrodynamically stretched at a cross junction under continuous flow. A supervised uniform manifold approximation and projection (UMAP) model is further developed for impedance‐based quantification of undifferentiated iPSCs with high sensitivity (≈1% spiked iPSCs) and shows good correlations with SCPCs differentiation outcomes using two iPSC lines. Cell membrane opacity (day 1) is also identified as a novel early intrinsic predictive biomarker that exhibits a strong correlation with SCPC differentiation efficiency (day 10). Overall, it is envisioned that this label‐free and optic‐free platform technology can be further developed as a versatile cost‐effective process analytical tool to monitor or assess stem cell quality and safety in regenerative medicine.

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Advancements in the Research of Modified Hydroxyapatite Materials for Bone Repair : a Scoping Review

Huang

Bai

et al. 2023

Preprint

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Modified hydroxyapatite (mHA) materials have shown efficacy in bone defect repair, exhibiting excellent properties such as biocompatibility, mechanical performance, antibacterial activity, and degradability. This article aims to consolidate existing research on the various aspects of mHA materials, discussing their superior performance and the fundamental principles of osteoinduction. Additionally, it explores the potential of these materials to facilitate the exploration of novel bone repair materials in the future. The primary objective of this study is to provide new insights into optimizing the functionality and structure of bone repair materials, as well as enhancing their biocompatibility, mechanical properties, antibacterial capabilities, and degradability. A systematic search was conducted in PubMed and Web of Science databases for relevant articles on modified hydroxyapatite materials. A total of 601 articles were retrieved, 461 articles with poor correlation with the purpose of the study, obsolete content, repetition and stage of the same author were excluded, and 140 articles that met the criteria were reviewed. The critical analysis and synthesis were based on the examination of in vitro and in vivo experimental results, and theoretical foundations of mHA materials. The discussion section addresses the current challenges and identifies future directions in this field.

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Understanding the interplay of membrane trafficking, cell surface mechanics, and stem cell differentiation

Cited by 4 publications

References 214 publications

Piezo1 activates noncanonical EGFR endocytosis and signaling

Piezo1 activates noncanonical EGFR endocytosis and signaling

Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling

Advancements in the Research of Modified Hydroxyapatite Materials for Bone Repair : a Scoping Review

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