Understanding the relationship between norovirus diversity and immunity

Ford-Siltz, Lauren A.; Tohma, Kentaro; Parra, Gabriel I.

doi:10.1080/19490976.2021.1900994

Cited by 33 publications

(24 citation statements)

References 106 publications

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“…A low infectious dose and easily transmissibility make challenging to control the noroviruses burden [ 5 ]. However, efforts to develop noroviruses vaccines were hindered due to multiple factors [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid screening for antigenic characterization of GII.17 norovirus strains with variations in capsid gene

Liao

Xue²,

Gao³

et al. 2022

Gut Pathog

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The emergence of the novel GII.17 Kawasaki 2014 norovirus variant raising the interest of the public, has replaced GII.4 as the predominant cause of noroviruses outbreaks in East Asia during 2014–2015. Antigenic variation of the capsid protein is considered as one of the key mechanisms of norovirus evolution. In this study, we screened a panel of GII.17 mutants. First, we produced norovirus P proteins using cell-free protein synthesis (CFPS) system, comparing the results to pure proteins expressed in a cell-based system. Next, we determined the binding capability of specific monoclonal antibody (mAb) 2D11 using a unique set of wild-type GII.17 strains. Results of the EIA involving a panel of mutant cell-free proteins indicated that Q298 was the key residue within loop 1. These data highlighted the essential residues in the linear antibody binding characteristics of novel GII.17. Furthermore, it supported the CFPS as a promising tool for rapidly screening mutants via the scalable expression of norovirus P proteins.

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Section: Introductionmentioning

confidence: 99%

Rapid screening for antigenic characterization of GII.17 norovirus strains with variations in capsid gene

Liao

Xue²,

Gao³

et al. 2022

Gut Pathog

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“…One of the major obstacles for norovirus vaccine development is the large viral genetic and antigenic diversity [10]. Genetic differences within VP1 have been used to classify noroviruses into genogroups and genotypes [11].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analyses of human noroviruses provide insights on evolutionary dynamics and evidence of coexisting viral populations evolving under recombination constraints

Tohma¹,

Lepore²,

Martínez

et al. 2021

PLoS Pathog

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Norovirus is a major cause of acute gastroenteritis worldwide. Over 30 different genotypes, mostly from genogroup I (GI) and II (GII), have been shown to infect humans. Despite three decades of genome sequencing, our understanding of the role of genomic diversification across continents and time is incomplete. To close the spatiotemporal gap of genomic information of human noroviruses, we conducted a large-scale genome-wide analyses that included the nearly full-length sequencing of 281 archival viruses circulating since the 1970s in over 10 countries from four continents, with a major emphasis on norovirus genotypes that are currently underrepresented in public genome databases. We provided new genome information for 24 distinct genotypes, including the oldest genome information from 12 norovirus genotypes. Analyses of this new genomic information, together with those publicly available, showed that (i) noroviruses evolve at similar rates across genomic regions and genotypes; (ii) emerging viruses evolved from transiently-circulating intermediate viruses; (iii) diversifying selection on the VP1 protein was recorded in genotypes with multiple variants; (iv) non-structural proteins showed a similar branching on their phylogenetic trees; and (v) contrary to the current understanding, there are restrictions on the ability to recombine different genomic regions, which results in co-circulating populations of viruses evolving independently in human communities. This study provides a comprehensive genetic analysis of diverse norovirus genotypes and the role of non-structural proteins on viral diversification, shedding new light on the mechanisms of norovirus evolution and transmission.

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“…In Japan, the United States (US) and Europe, NoV accounts for 50% of all food-related illness (Thébault et al, 2021). Moreover, the global socioeconomic burden from NoV is estimated to be around 4.2 billion US Dollars (USD) (equivalent to 3.0 billion pounds) in direct health-care costs, with an additional 60.3 billion USD annually in indirect costs (equivalent to 47.8 billion pounds) (Ford-Siltz et al, 2021). NoV is estimated to cause 685 million cases and 210,000 deaths worldwide every year (Cannon et al, 2021;Cates et al, 2020;) .…”

Section: Introductionmentioning

confidence: 99%

A socio-technical approach to food safety incident analysis using the AcciMap model in the hospitality sector

et al. 2022

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Understanding the relationship between norovirus diversity and immunity

Cited by 33 publications

References 106 publications

Rapid screening for antigenic characterization of GII.17 norovirus strains with variations in capsid gene

Rapid screening for antigenic characterization of GII.17 norovirus strains with variations in capsid gene

Genome-wide analyses of human noroviruses provide insights on evolutionary dynamics and evidence of coexisting viral populations evolving under recombination constraints

A socio-technical approach to food safety incident analysis using the AcciMap model in the hospitality sector

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