Online activities are a fundamental part of daily life in this digital era and Internet self-efficacy (ISE) became a central construct for the psychologists of virtual environments. The Internet Self-Efficacy Scale developed by Kim and Glassman (2013) is a recent, valid, and reliable 17-item test to assess the ISE. The aim of this research, composed by two studies, was to translate and validate the Italian version of the ISS. In study 1, we evaluated the factorial validity and internal consistency of the Italian version of the ISS on 3724 individuals. In study 2, we tested the ISS external validity relying on the Bergen Social Media Addiction Scale (BSMAS) and the Internet Addiction Scale (IAS) based on the answers of 244 participants. The findings supported the reliability and validity of the translated ISS and its use for assessing ISE on the Italian population. Implications for researchers and interventions are also discussed.