Unexpected sensitivity enhancement in analysing alfatoxin M1 using LC-IDMS

Tölgyesi, Ádám; Kovács, Bence Tóth; Tóth, Edgár; Simon, Ágnes; Bálint, Mária; Sharma, Virender K.

doi:10.1016/j.microc.2022.107469

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…In our earlier studies, we found that the mobile phase did not require acidic conditions to obtain high sensitivity for the analysis of pesticides and mycotoxins using LC-MS/MS separation and employing positive ionization [ 37 , 39 ]. The response of DON, aflatoxins and some pesticides (e.g., chlorpyrifos-ethyl/methyl), detected as a protonated molecule ion, slightly increased at alkaline pH in comparison with acidic conditions ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Development of a Novel LC-MS/MS Multi-Method for the Determination of Regulated and Emerging Food Contaminants Including Tenuazonic Acid, a Chromatographically Challenging Alternaria Toxin

Tölgyesi¹,

Cseh²,

Simon³

et al. 2023

Molecules

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The regulation of food contaminants in the European Union (EU) is comprehensive, and there are several compounds in the register or being added to the recommendation list. Recently, European standard methods for analysis have also been issued. The quick analysis of different groups of analytes in one sample requires a number of methods and the simultaneous use of various instruments. The aim of the present study was to develop a method that could analyze several groups of food contaminants: in this case, 266 pesticides, 12 mycotoxins, 14 alkaloid toxins, and 3 Alternaria toxins. The main advantage of the herein described approach over other methods is the simultaneous analysis of tenuazonic acid (TEA) and other relevant food contaminants. The developed method unites the newly published standard methods such as EN 15662:2018, EN 17194:2019, EN 17256:2019, EN 17425:2021, EN 17521:2021, which describes the analysis of both regulated and emerging contaminants. The developed method is based on a QuEChERS sample preparation, followed by LC-MS/MS analysis under alkaline mobile phase conditions. The pH of the aqueous eluent was set to 8.3, which resulted in baseline separation among ergot alkaloids and their corresponding epimers, a symmetric chromatographic peak shape for analyzing TEA and fit-for-purpose sensitivity for MS/MS detection in both positive and negative ionization modes. Those compounds, which possess the corresponding isotopically labeled internal standards (ISTD), allowed for direct quantification by the developed method and no further confirmation was necessary. This was proven by satisfactory analyses of a number of quality control (QC), proficiency test (PT), and validation samples.

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Section: Resultsmentioning

confidence: 99%

Development of a Novel LC-MS/MS Multi-Method for the Determination of Regulated and Emerging Food Contaminants Including Tenuazonic Acid, a Chromatographically Challenging Alternaria Toxin

Tölgyesi¹,

Cseh²,

Simon³

et al. 2023

Molecules

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“…Nowadays, there are many methods for detection AFM1 in milk and dairy products. Based on the reviewed literature, there are Thin-Layer Chromatography (TLC) [14,15]with good performance in bioassay and chromatography, the Enzyme-linked immunosorbent assay with obvious specificity for AFM1 [16][17][18], the Fluorescence Spectrophotometry with easy use [19], low price and fast, and the most commonly used and stable combination of liquid chromatography and liquid chromatography mass spectrometry [8,10,20]. However, as a method for the rapid detection of mycotoxins, thin-layer chromatography (TLC) is simple and quick, but experimental repeatability and precision are not well; The enzyme immune assay has a strong specificity for AFTs, but the rate of false positives was high, so more accurate detection methods are also used to facilitate detection; Liquid chromatography is stable and sensitive, but it could not detect and distinguish homologues or isomers with similar structures at the same time.…”

Section: Introductionmentioning

confidence: 99%

Certification of a novel matrix reference material for accurate measurements of aflatoxin M1 in milk powder

Huang,

Liu,

Zhou

et al. 2024

Accred Qual Assur

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In this study, two different concentrations of matrix certified reference materials (CRMs) were produced for the accurate measurement of aflatoxin M1(AFM1) in milk powder((GBW(E) 100552, GBW(E) 100553)). The raw material was obtained by feeding cows with positive drugs. The homogeneity, stability and characterization of this matrix CRMs were examined by liquid chromatography tandem mass spectrometry with isotope-labeled internal standard method. The certified value for the low concentration of AFM1 in milk powder was 2.45 µg/kg with an uncertainty of 0.43 µg/kg (coverage factor k=2, at 95% confidence). The certified value for the high concentration of AFM1 in milk powder was 3.45 µg/kg with an expanded uncertainty of 0.49 µg/kg (coverage factor k=2, at 95% confidence). In addition, the samples were evaluated in detail for homogeneity, long-term stability at -80°C for 6 months, and short-term stability at 4°C for 7 days. The results showed that the samples were stable under the above conditions.

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Ultrasensitive ratiometric electrochemical aptasensor based on novel MnCo@C as nonenzyme catalysis for the detection of patulin

Liu,

Lai,

Ming

et al. 2024

Sensors and Actuators B: Chemical

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Unexpected sensitivity enhancement in analysing alfatoxin M1 using LC-IDMS

Cited by 5 publications

References 42 publications

Development of a Novel LC-MS/MS Multi-Method for the Determination of Regulated and Emerging Food Contaminants Including Tenuazonic Acid, a Chromatographically Challenging Alternaria Toxin

Development of a Novel LC-MS/MS Multi-Method for the Determination of Regulated and Emerging Food Contaminants Including Tenuazonic Acid, a Chromatographically Challenging Alternaria Toxin

Certification of a novel matrix reference material for accurate measurements of aflatoxin M1 in milk powder

Ultrasensitive ratiometric electrochemical aptasensor based on novel MnCo@C as nonenzyme catalysis for the detection of patulin

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