UNFERTILIZED EMBRYO SAC 12 phosphorylation plays a crucial role in conferring salt tolerance

He, Zihang; Wang, Zhen; Nie, Xiaowei; Qu, Ming; Zhao, Huimin; Ji, Xiaoyu; Wang, Yucheng

doi:10.1093/plphys/kiab549

Cited by 15 publications

(8 citation statements)

References 45 publications

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“…This is consistent with the findings of a previous study on pepper bHLH ( Liu et al, 2021 ). Arabidopsis AtUNE12 belongs to the bHLH TF superfamily, which can be induced by NaCl, mannitol and ABA to confer salt and osmotic stress tolerance in plants ( He et al, 2021 ). In the present study, the expression levels of eight candidate SabHLH genes were all increased under NaCl treatment, especially SabHLH038 (homologous to AtUNE12 ) was significantly upregulated under both NaCl and PEG treatments, and may play a role similar to that of AtUNE12 in S. aralocaspica .…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy

2022

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Basic helix-loop-helix (bHLH) transcription factors play important roles in plant growth, development, metabolism, hormone signaling pathways, and responses to abiotic stresses. However, comprehensive genomic and functional analyses of bHLH genes have not yet been reported in desert euhalophytes. Suaeda aralocaspica, an annual C4 halophyte without Kranz anatomy, presents high photosynthetic efficiency in harsh natural habitats and is an ideal plant for identifying transcription factors involved in stress resistance. In this study, 83 bHLH genes in S. aralocaspica were identified and categorized into 21 subfamilies based on conserved motifs, gene structures, and phylogenetic analysis. Functional annotation enrichment revealed that the majority of SabHLHs were enriched in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in the response to stress conditions, as transcription factors. A number of cis-acting elements related to plant hormones and stress responses were also predicted in the promoter regions of SabHLHs, which were confirmed by expression analysis under various abiotic stress conditions (NaCl, mannitol, low temperature, ABA, GA3, MeJA, and SA); most were involved in tolerance to drought and salinity. SabHLH169 (076) protein localized in the nucleus was involved in transcriptional activity, and gene expression could be affected by different light qualities. This study is the first comprehensive analysis of the bHLH gene family in S. aralocaspica. These data will facilitate further characterization of their molecular functions in the adaptation of desert plants to abiotic stress.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy

2022

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“…These results were consistent with previous reports. For instance, Arabidopsis AtATAF1 enhances drought tolerance by reducing stomatal aperture (Wu et al, 2009), AGL16 negatively modulates drought tolerance via stomatal movement in Arabidopsis (Zhao et al, 2020), AtUNE12 confers salt tolerance by decreasing stomatal aperture in Arabidopsis (He et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Salicylic acid-related ribosomal protein CaSLP improves drought andPst. DC3000tolerance in pepper

Zhang

Pei

Saeed

et al. 2022

Preprint

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The ribosomal protein SA plays an essential role in multiple aspects and is involved in plant growth and response to various stresses. Drought threatens pepper yield and quality. However, the resistance mechanism of pepper in response to drought are complex and not yet fully understood. Here, we describe the role of CaSLP in mediating pepper tolerance to drought stress. we found that CaSLP was highly expressed under drought and salicylic acid (SA) stress, and CaSLP was localized in cell nucleus and cytomembrane. Knockout of CaSLP gene significantly decreased the pepper drought tolerance, while transient expression of CaSLP leads to drought tolerance in pepper, and overexpression of the CaSLP dramatically increased the drought stress tolerance in Arabidopsis. Furthermore, exogenous spring salicylic acid enhanced drought tolerance. The characterization of resistance molecular mechanisms in the Pseudomonas syringae pv. Tomato DC3000 (Pst.DC3000) is of great significance for the pepper yield and quality, we found that CaSLP-knockdown pepper plants demonstrated decreased Pst.DC3000 tolerance, whereas ectopic expression of the CaSLP increased the Pst.DC3000 stress tolerance in Arabidopsis. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) results showed that CaNAC035 physically interacts with CaSLP in the cell nucleus, and the CaNAC035 was identified as an upstream partner of the CaPR1 promoter and activated the transcription. Taken together, our data demonstrated that CaSLP plays an essential role in the regulation of drought stress. Our study elucidates the roles of CaSLP response to drought stress tolerance. Furthermore, a possible regulatory model and molecular mechanisms under drought stress is proposed.

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“…The expression levels of AtRD29B , AtLEA6 , AtLEA7 , AtERD1 , AtP5CS1 , AtSOS1 , AtSOS2 , and AtSOS3 were investigated using RT-qPCR. The treatment methods were conducted as previously described [ 2 ]. Three biological replicates were used to detect changes in gene expression.…”

Section: Methodsmentioning

confidence: 99%

“…Stress tolerance of plants is influenced by the expression of stress-induced genes regulated by specific transcription factors (TFs) [ 1 ]. TFs directly or indirectly regulate the expression of downstream stress-related target genes via cis-acting elements [ 1 , 2 ]. The bZIP transcription factor is one of the key regulators of plant stress resistance involved in regulating the defense against pathogens, light tolerance, and stress-signal transduction [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat

Weng

Lü

Zhou

et al. 2022

IJMS

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ABFs play a key role in regulating plant osmotic stress. However, in Tartary buckwheat, data on the role of ABF genes in osmotic stress remain limited and its associated mechanism in osmoregulation remain nebulous. Herein, a novel ABF family in Tartary buckwheat, FtbZIP12, was cloned and characterized. FtbZIP12 is a transcriptional activator located in the nucleus; its expression is induced by NaCl, mannitol, and abscisic acid (ABA). Atopic expression of FtbZIP12 in Arabidopsis promoted seed germination, reduced damage to primary roots, and improved the tolerance of seedlings to osmotic stress. The quantitative realtime polymerase chain reaction (RT-qPCR) results showed that the expressions of the typical genes related to stress, the SOS pathway, and the proline synthesis pathway in Arabidopsis were significantly (p < 0.05) upregulated under osmotic stress. FtbZIP12 improved the osmotic pressure resistance by reducing the damage caused by reactive oxygen species to plants and maintained plant homeostasis by upregulating the expression of genes related to stress, osmotic regulation, and ion homeostasis. This study identified a key candidate gene for understanding the mechanism underlying osmotic-stress-regulated function in Tartary buckwheat, thereby providing a theoretical basis for improving its yield and quality.

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UNFERTILIZED EMBRYO SAC 12 phosphorylation plays a crucial role in conferring salt tolerance

Cited by 15 publications

References 45 publications

Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy

Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family in Suaeda aralocaspica, an Annual Halophyte With Single-Cell C4 Anatomy

Salicylic acid-related ribosomal protein CaSLP improves drought andPst. DC3000tolerance in pepper

FtbZIP12 Positively Regulates Responses to Osmotic Stress in Tartary Buckwheat

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