Uniaxial and Coaxial Nanofibers PCL/Alginate or PCL/Gelatine Transport and Release Tamoxifen and Curcumin Affecting the Viability of MCF7 Cell Line

Abstract

Breast cancer is the second cause of cancer death in women worldwide. The search for therapeutic and preventive alternatives has increased in recent years. One synthetic drug for patients with hormone receptor-positive tumours is tamoxifen citrate (TMX). Curcumin (Cur) is a natural compound that is being tested. Both were coupled with nanoscale-controlled and sustained release systems to increase the effectiveness of the treatment and reduce adverse effects. We produced a controlled release system based on uniaxial and coaxial polymeric nanofibers of polycaprolactone (PCL), alginate (Alg) and gelatine (Gel) for the transport and release of TMX and Cur, as a new alternative to breast cancer treatment. Nanofibers combining PCL–Alg and PCL–Gel were fabricated by the electrospinning technique and physicochemically characterised by thermal analysis, absorption spectroscopy in the infrared region and X-ray diffraction. Morphology and size were studied by scanning electron microscopy. Additionally, the release profile of TMX and Cur was obtained by UV-Vis spectroscopy. Additionally, the cytotoxic effect on breast cancer cell line MCF7 and peripheral-blood mononuclear cells (PBMCs) from a healthy donor were evaluated by a Resazurin reduction assay. These assays showed that PCL–TMX nanofiber was highly toxic to both cell types, while PCL–Cur was less toxic.

One-step Preparation of Antibacterial Gelatin/Polycaprolactone Nanofibrous Janus Membranes for Efficient Hemostasis

“…PCL particles (20 wt %) were added into the mixed solution of dichloromethane and methanol (volume ratio of 1:1) and stirred for 6 h until completely dissolved according to a previous work. 25 Then, the Gel@Ze@PDA mixed solution and the PCL solution were separately placed in two parallel troughs connected with a voltage of 80 kV, and the distance was 23 cm. Thus, the two-layer membrane was obtained from the receiving end by electrospinning in turn, wherein a pure cotton non-woven fabric was used as the substrate.…”

Antimicrobial peptide immobilization on catechol-functionalized PCL/alginate wet-spun fibers to combat surgical site infection

“…S4(c) (ESI †). 70 However, according to the XRD data of PCL/Alg, its microstructure was mostly similar to PCL because of the presence of similar diffraction peaks. This proves that Alg chains were encapsulated within PCL molecules.…”

Surface Functionalization of Nanofibers: The Multifaceted Approach for Advanced Biomedical Applications

“…Nanofibers have attracted considerable massive attention and emerged as novel nanomaterials due to potential physicochemical properties, surfactant free and, commercial potential [ 1 , 2 ]. Their advanced applications can be attributed to the ability to form network of highly porous mesh with notable interlinking amongst pores [ 3 ].…”