Uniaxial Tensile Testing Device for Measuring Mechanical Properties of Biological Tissue with Stress-Relaxation Test under a Confocal Microscope

Vondrášek, David; Hadraba, Daniel; Matějka, Roman; Lopot, František; Svoboda, Martin; Jelen, Karel

doi:10.21062/ujep/192.2018/a/1213-2489/mt/18/5/866

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…A coating of this type may prevent acute thrombus formation while endothelial cells adhere and proliferate on the surface of the graft. The suppression of thrombus formation and the increased proliferation of saphenous vein endothelial cells in vitro on polyvinyl chloride (PVC) coated with a fibrin-heparin mesh was reported in an earlier publication [34].…”

Section: Discussionmentioning

confidence: 81%

“…The results indicate that SDS treatment maintains the range of Young's modulus and the mechanical anisotropy trends of porcine pericardium. Two perpendicular orientations from native, SDS 1% treated and SDS 0.5% treated samples were tested for their mechanical response, adopting the stress relaxation protocol [34]. This protocol reveals the elastic response of viscoelastic DP, which is represented by the Young's modulus.…”

Section: Discussionmentioning

confidence: 99%

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Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

et al. 2021

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An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity of the graft is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling of the scaffold by the patient’s cells should result in the formation of living autologous tissue. In the work presented here, decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor (VEGF) to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells (ASCs). While the ASCs grew only on the surface of the decellularized pericardium, the fibrin-modified scaffolds were entirely repopulated in 28 d, and the scaffolds modified with fibrin, heparin and VEGF were already repopulated within 6 d. Label free mass spectrometry revealed fibronectin, collagens, and other extracellular matrix proteins produced by ASCs during recellularization. Thin layers of human umbilical endothelial cells were formed within 4 d after the cells were seeded on the surfaces of the scaffold, which had previously been seeded with ASCs. The results indicate that an artificial tissue prepared by in vitro recellularization and remodeling of decellularized non-autologous pericardium with autologous ASCs seems to be a promising candidate for cardiovascular grafts capable of accelerating in situ endothelialization. ASCs resemble the valve interstitial cells present in heart valves. An advantage of this approach is that ASCs can easily be collected from the patient by liposuction.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

et al. 2021

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“…There are a limited number of studies in the literature determining linear properties like Young’s modulus and Poisson’s ratio of ePTFE (Faturechi et al 2019 ; Bouchet et al 2019 ), Dacron (Faturechi et al 2019 ; Coirbay 2020 ), porcine (Aguiari et al 2016 ; Vondrášek et al 2018 ; Inoue et al 2021 ) and bovine pericardia (Zioupos and Barbenel 1994 ; Aguiari et al 2016 ) through mechanical tensile tests, as these materials are examined in the framework of viscoelastic theory. However, the experimental measurements in these studies were conducted over different strain ranges, rendering direct comparisons with our values unfeasible.…”

Section: Discussionmentioning

confidence: 99%

Mechanical characterization and torsional buckling of pediatric cardiovascular materials

Donmazov,

Piskin,

Gölcez

et al. 2024

Biomech Model Mechanobiol

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In complex cardiovascular surgical reconstructions, conduit materials that avoid possible large-scale structural deformations should be considered. A fundamental mode of mechanical complication is torsional buckling which occurs at the anastomosis site due to the mechanical instability, leading surgical conduit/patch surface deformation. The objective of this study is to investigate the torsional buckling behavior of commonly used materials and to develop a practical method for estimating the critical buckling rotation angle under physiological intramural vessel pressures. For this task, mechanical tests of four clinically approved materials, expanded polytetrafluoroethylene (ePTFE), Dacron, porcine and bovine pericardia, commonly used in pediatric cardiovascular surgeries, are conducted (n = 6). Torsional buckling initiation tests with n = 4 for the baseline case (L = 7.5 cm) and n = 3 for the validation of ePTFE (L = 15 cm) and Dacron (L = 15 cm and L = 25 cm) for each are also conducted at low venous pressures. A practical predictive formulation for the buckling potential is proposed using experimental observations and available theory. The relationship between the critical buckling rotation angle and the lumen pressure is determined by balancing the circumferential component of the compressive principal stress with the shear stress generated by the modified critical buckling torque, where the modified critical buckling torque depends linearly on the lumen pressure. While the proposed technique successfully predicted the critical rotation angle values lying within two standard deviations of the mean in the baseline case for all four materials at all lumen pressures, it could reliably predict the critical buckling rotation angles for ePTFE and Dacron samples of length 15 cm with maximum relative errors of 31% and 38%, respectively, in the validation phase. However, the validation of the performance of the technique demonstrated lower accuracy for Dacron samples of length 25 cm at higher pressure levels of 12 mmHg and 15 mmHg. Applicable to all surgical materials, this formulation enables surgeons to assess the torsional buckling potential of vascular conduits noninvasively. Bovine pericardium has been found to exhibit the highest stability, while Dacron (the lowest) and porcine pericardium have been identified as the least stable with the (unitless) torsional buckling resistance constants, 43,800, 12,300 and 14,000, respectively. There was no significant difference between ePTFE and Dacron, and between porcine and bovine pericardia. However, both porcine and bovine pericardia were found to be statistically different from ePTFE and Dacron individually (p < 0.0001). ePTFE exhibited highly nonlinear behavior across the entire strain range [0, 0.1] (or 10% elongation). The significant differences among the surgical materials reported here require special care in conduit construction and anastomosis design.

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“…This device is in principle similar to Luggage Wheel Wear Test Machine [17], but has simpler design and was built from standard widely available components and materials. Other ideas dealing with similar issues can be found in [14][15][16][17][18][19][20].…”

Section: Testingmentioning

confidence: 99%

Experimental Measurements on a Stand for a Grain Sampler

Cézová¹,

Lopot²,

Machac³

et al. 2022

Manufacturing Technology

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This paper describes experimental measurements of travel rollers on a polyurethane thread. This experiment took place at the CTU Faculty of Mechanical Engineering in the laboratory of the Department of Design and Machine Parts. The experiment was performed within the project of a lightweight type of sampler, designed by a team of collaborators from our institute for OK Servis BioPro, s.r.o. For the newly designed type of sampler, it was necessary to determine the operation of a newly designed grain sampler. The load of the rollers is different in each environment. This paper presents the average values from the measurement, including its evaluation. Moreover, the paper compares different temperatures that can be achieved in the practice. Negative temperature values were not performed, as this measurement would be expensive and inefficient. Grain samplerExperiment Measurement Travel roll Polyurethane

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Uniaxial Tensile Testing Device for Measuring Mechanical Properties of Biological Tissue with Stress-Relaxation Test under a Confocal Microscope

Cited by 8 publications

References 35 publications

Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Mechanical characterization and torsional buckling of pediatric cardiovascular materials

Experimental Measurements on a Stand for a Grain Sampler

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