Unigene-Derived Microsatellite Marker Based Variation Study of Phytophthora nicotianae Isolates Infecting Citrus

Nerkar, S.; Bawage, Swapnil; Kumar, Ashok; Das, A. K.

doi:10.1007/s12088-011-0244-9

Cited by 3 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, in the present study, P. palmivora and P. nicotianae were characterized through the amplification of ITS region that can rapidly distinguish between the two main species of Phytophthora involved in causing diseases in citrus [22]. The phylogenetic analysis based on ITS region revealed that P. palmivora and P. nicotianae isolates closely clustered with respective Phytopthora species infecting different citrus species in India and other different countries [9,31]. Thus, the study clearly showed that Phytophthora isolates collected from 31 places of Kodagu district confirmed the presence of two Phytophthora species, namely P.…”

Section: Discussionmentioning

confidence: 99%

Status of Phytophthora and Huanglongbing Diseases of Citrus Causing Decline in Coorg Mandarin in India

Sonavane¹,

Venkataravanappa²

2017

JAST-B

View full text Add to dashboard Cite

Coorg mandarin is a famous ecotype of mandarin grown in multi-tier cropping system in coffee and pepper plantations in Coorg region of India. The roving survey indicated disease incidence of Huanglongbing (HLB) and Phytophthora, ranging between 35%-64.2% and 22.5%-35.6% in different places of Coorg region. A total of 177 Phytophthora infected (plant roots 59, soil 118), and 576 greening or HLB infected Coorg mandarin samples were collected from 184 Coorg mandarin orchards, of these 111 Phytophthora isolates were isolated and characterized in this study on the basis of colony growth patterns and morphological structures. Based on the virulence, only 52 of 111 Phytophthora isolates were amplified by PCR using universal internal transcriber spacer (ITS) primers, cloned and sequenced. The sequences analysis of Phytophthora isolates revealed more than 97% nucleotide (nt) similarity within themselves, except eight isolates of P. palmivora and four isolates of P. nicotianae had varied nt identity (70.6% to 97.4%) with other Phytophthora species compared. Similarly, 523 of 576 Coorg mandarin samples were confirmed the presence of HLB infection by PCR using 16S rRNA gene specific universal primers. The representative five of 523 Coorg mandarin isolates showed nt identity ranged from 94.4% to 95.3% with Candidatus Liberibacter asiaticus (AB008366) isolate infecting citrus in Asia and one isolate of HLB shared nt identity of 96.9% with Ca. L. asiaticus (KJ944269).

show abstract

Section: Discussionmentioning

confidence: 99%

Status of Phytophthora and Huanglongbing Diseases of Citrus Causing Decline in Coorg Mandarin in India

Sonavane¹,

Venkataravanappa²

2017

JAST-B

View full text Add to dashboard Cite

show abstract

Characterization of Phytophthora species inciting citrus foot rot in Punjab

Kaur,

Hunjan,

Arora

et al. 2024

Indian Phytopathology

View full text Add to dashboard Cite

Diversity of citrus gummosis in São Paulo State, Brazil

2016

View full text Add to dashboard Cite

Citrus gummosis is an important disease because it causes the death of young plants, resulting in their replacement, a costly operation. Its causal agents are different species of Phytophthora. Dissemination of these microorganisms occurs mainly by contaminated nursery trees. Differences in aggressiveness, competitiveness and response to fungicides varied among species of this pathogen. Therefore, this study aimed to examine diversity among citrus isolates of Phytophthora from two different origins in São Paulo State. Thirty isolates from citrus nursery trees from 13 municipalities and 11 from seven commercial orchards were analyzed. Interspecific diversity was performed by species-specific primers and sequencing of the ITS region. Subsequently, intraspecific diversity was carried out with 16 RAPD primers and clustering analysis of UPGMA, using the Dice coefficient. Our data identified only P. nicotianae from either substrates or commercial fields. Isolates clustered into two genetically distinct populations. Genetically similar isolates were also found. The existence of these clonal lineages among isolates from geographically distinct nursery trees suggests an efficient dissemination mechanism. This was the first study to examine the diversity of Phytophthora in citrus from substrates of nursery trees in Brazil.

show abstract

Unigene-Derived Microsatellite Marker Based Variation Study of Phytophthora nicotianae Isolates Infecting Citrus

Cited by 3 publications

References 8 publications

Status of Phytophthora and Huanglongbing Diseases of Citrus Causing Decline in Coorg Mandarin in India

Status of Phytophthora and Huanglongbing Diseases of Citrus Causing Decline in Coorg Mandarin in India

Characterization of Phytophthora species inciting citrus foot rot in Punjab

Diversity of citrus gummosis in São Paulo State, Brazil

Contact Info

Product

Resources

About