Unique guanine nucleotide binding properties of the human placental GTP-binding protein Gp

Tamir, Amichai; Fawzi, Ahmad; Northup, John K.

doi:10.1021/bi00482a002

Cited by 8 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our maximal estimate for the intrinsic hydrolysis rate of the SRP (<0.002 min -t) is comparable to the extremely low hydrolysis rates reported for the Sec4 protein (Kabcenell et al, 1990) and the ras p21 protein (Gibbs et al, 1984). The intrinsic hydrolysis rate for the SRP receptor (0.02 min -~) was lower than the value of 0.4 min -1 reported for Gs (Brandt and Ross, 1985), but comparable to rates reported for Gi (Sunyer et al, 1984) and Gp (Tamir et al, 1990). We were not able to detect a significant amount of protein bound ribonucleotide when the SRP receptor was incubated with GTP.…”

Section: Discussionsupporting

confidence: 74%

GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor.

Connolly

Gilmore

1993

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Translocation of proteins across the endoplasmic reticulum membrane is a GTP-dependent process. The signal recognition particle (SRP) and the SRP receptor both contain subunits with GTP binding domains. One GTP-dependent reaction during protein translocation is the SRP receptor-mediated dissociation of SRP from the signal sequence of a nascent polypeptide. Here, we have assayed the SRP and the SRP receptor for GTP binding and hydrolysis activities. GTP hydrolysis by SRP was not detected, so the maximal GTP hydrolysis rate for SRP was estimated to be <0.002 mol GTP hydrolyzed x mol of SRP -1 x min-'. The intrinsic GTP hydrolysis activity of the SRP receptor ranged between 0.02 and 0.04 mol GTP hydrolyzed × mol of SRP receptor -l x min -1. A 40-fold enhancement of GTP hydrolysis activity relative to that observed for the SRP receptor alone was obtained when complexes were formed between SRP and the SRP receptor. GTP hydrolysis activity was inhibited by GDP, but not by ATE Extended incubation of the SRP or the SRP receptor with GTP resulted in substoichiometric quantities of protein-bound ribonucleotide. SRP-SRP receptor complexes engaged in GTP hydrolysis were found to contain a minimum of one bound guanine dbonucleotide per SRP-SRP receptor complex. We conclude that the GTP hydrolysis activity described here is indicative of one of the GTPase cycles that occur during protein translocation across the endoplasmic reticulum.

show abstract

Section: Discussionsupporting

confidence: 74%

GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor.

Connolly

Gilmore

1993

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…The proteins encoded by these genes were reported to function in signal transduction pathways. Defects in this gene are implicated in many cancers, such as bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma [117,118].…”

Section: Discussionmentioning

confidence: 99%

Genetic polymorphisms and breast cancer risk: evidence from meta-analyses, pooled analyses, and genome-wide association studies

Peng

Lü

Ruan

et al. 2011

Breast Cancer Res Treat

View full text Add to dashboard Cite

To address the association between variants and breast cancer, an increasing number of articles on genetic association studies, genome-wide association studies (GWASs), and related meta- and pooled analyses have been published. Such studies have prompted an updated assessment of the associations between gene variants and breast cancer risk. We searched PubMed, Medline, and Web of Science and retrieved a total of 87 meta- and pooled analyses, which addressed the associations between 145 gene variants and breast cancer. Analyses met the following criteria: (1) breast cancer was the outcome, (2) the articles were all published in English, and (3) in the recent published meta- and pooled analyses, the analyses with more subjects were selected. Among the 145 variants, 46 were significantly associated with breast cancer and the other 99 (in 62 genes) were not significantly associated with breast cancer. The summary ORs for the 46 significant associations (P < 0.05) were further assessed by the method of false-positive report probability (FPRP). Our results demonstrated that 10 associations were noteworthy: CASP8 (D302H), CHEK2 (*1100delC), CTLA4 (+49G>A), FGFR2 (rs2981582, rs1219648, and rs2420946), HRAS (rare alleles), IL1B (rs1143627), LSP1 (rs3817198), and MAP3K1 (rs889312). In addition, eight GWASs were identified, in which 25 loci were obtained (14 in nine genes, six near a gene or genes, and five intergenic loci). Of the 25 SNPs, 20 were noteworthy: C6orf97 (rs2046210 and rs3757318), FGFR2 (rs2981579, rs1219648, and rs2981582), LSP1 (rs909116), RNF146 (rs2180341), SLC4A7 (rs4973768), MRPS30 (rs7716600), TOX3 (rs3803662 and rs4784227), ZNF365 (rs10995190), rs889312, rs614367, rs13281615, rs13387042, rs11249433, rs1011970, rs614367, and rs1562430. In summary, in this review of genetic association studies, 31.7% of the gene-variant breast cancer associations were significant, and 21.7% of these significant associations were noteworthy. However, in GWASs, 80% of the significant associations were noteworthy.

show abstract

“…The basis of our kinetic approach is to use well-defined nucleotide complexes of p21 in the absence of excess nucleotide, so that processes of interest occur under single-turnover conditions. The advantage of this approach is that observed rate constants are independent of the concentration of native p21 rm, a value usually based on filter-binding assays which are subject to systematic errors [8].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence approaches to the study of the p21ras GTPase mechanism

Eccleston¹,

Moore²,

Brownbridge³

et al. 1991

Biochemical Society Transactions

View full text Add to dashboard Cite

The use of ribose-modified guanine nucleotides and tryptophan mutants of p21ras, neither of which have significant effect on the kinetic mechanism of the p21ras GTPase and the GAP-activated p21ras GTPase, will now allow a detailed kinetic study of how GAP and other regulatory proteins interact with p21ras. This will lead to a better understanding of how the relative concentrations of 'active' p21ras. GTP and 'inactive' p21ras. GDP are regulated in the cell.

show abstract

Unique guanine nucleotide binding properties of the human placental GTP-binding protein Gp

Cited by 8 publications

References 28 publications

GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor.

GTP hydrolysis by complexes of the signal recognition particle and the signal recognition particle receptor.

Genetic polymorphisms and breast cancer risk: evidence from meta-analyses, pooled analyses, and genome-wide association studies

Fluorescence approaches to the study of the p21ras GTPase mechanism

Contact Info

Product

Resources

About