Unique self-assembly properties of a bridge-shaped protein dimer with quantum dots

Wang, Jianhao; Jiang, Pengju; Gao, Liqian; Yu, Yongsheng; Lu, Yao; Qiu, Lin; Wang, Cheli; Xia, Junfeng

doi:10.1007/s11051-013-1914-9

Cited by 20 publications

(20 citation statements)

References 46 publications

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“…S4 †). 21 Interestingly, the rebinding step was observed aer the displacement step. Aer adding imidazole for 15 min, the intermediates QD-(PC 10 A) m -(imidazole) n were replaced with the QD-(PC 10 A) 25 nanogel.…”

Section: Loading Of Hybrid Nanogel With Drugsmentioning

confidence: 99%

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Yang

Yao

et al. 2014

Nanoscale

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A new type of multifunctional quantum dot (QD)-polypeptide hybrid nanogel with targeted imaging and drug delivery properties has been developed by metal-affinity driven self-assembly between artificial polypeptides and CdSe-ZnS core-shell QDs. On the surface of QDs, a tunable sandwich-like microstructure consisting of two hydrophobic layers and one hydrophilic layer between them was verified by capillary electrophoresis, transmission electron microscopy, and dynamic light scattering measurements. Hydrophobic and hydrophilic drugs can be simultaneously loaded in a QD-polypeptide nanogel. In vitro drug release of drug-loaded QD-polypeptide nanogels varies strongly with temperature, pH, and competitors. A drug-loaded QD-polypeptide nanogel with an arginine-glycine-aspartic acid (RGD) motif exhibited efficient receptor-mediated endocytosis in αvβ3 overexpressing HeLa cells but not in the control MCF-7 cells as analyzed by confocal microscopy and flow cytometry. In contrast, non-targeted QD-polypeptide nanogels revealed minimal binding and uptake in HeLa cells. Compared with the original QDs, the QD-polypeptide nanogels showed lower in vitro cytotoxicity for both HeLa cells and NIH 3T3 cells. Furthermore, the cytotoxicity of the targeted QD-polypeptide nanogel was lower for normal NIH 3T3 cells than that for HeLa cancer cells. These results demonstrate that the integration of imaging and drug delivery functions in a single QD-polypeptide nanogel has the potential for application in cancer diagnosis, imaging, and therapy.

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Section: Loading Of Hybrid Nanogel With Drugsmentioning

confidence: 99%

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Yang

Yao

et al. 2014

Nanoscale

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“…Furthermore, CE-FL can provide far more detailed information on QDs-protein assembly than ensemble fluorescence measurement [15]. …”

Section: Resultsmentioning

confidence: 99%

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

Qiu

Wang

et al. 2014

IJMS

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In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.

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“…The shape of the protein (e.g. monomeric vs rigid bridge-shaped dimeric structures) itself also influences the saturation ratio [39]. Banerjee et al (2015) [28] investigated the effect of the length and the use of double or single stranded DNA.…”

Section: Packing Of Biomolecules On Qdsmentioning

confidence: 99%

“…Despite the relatively high affinity between metal and His-tag, QD-protein assemblies are still prone to displacement by competitive ligands, such as imidazole, glutathione (GSH), and His-tag peptides. As biological fluids often contain metal-binding molecules and proteins, ligand displacement will result in mixed ligands on QDs, and hamper their use in tissue imaging or drug delivery [39]. Although His n -QD surface interactions have been postulated for some time, the first description in literature was in 2005 by Medintz and co-workers [52,65].…”

Section: Dative Chemistrymentioning

confidence: 99%

Bioconjugation of quantum dots: Review & impact on future application

Foubert

Beloglazova

Rajković

et al. 2016

TrAC Trends in Analytical Chemistry

120

110

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Nowadays luminescent semiconductor quantum dots (QDs) are widely applied in different areas due to their unique optical properties. QDs can be used as photoluminescent labels with excellent possibilities for high-throughput detection and diagnostics. For most of such applications QDs must be coupled to biomolecules, which often represents a fundamental challenge. Although QDs have a lot of advantages over organic dyes, most of the techniques that have been developed for QD functionalization and bioconjugation, are more complicated than the corresponding techniques for organic fluorescent dyes. Here, the importance of choosing a suitable bioconjugation strategy in different applications, such as imaging and assays is described. The main goal of this review is to give a structured and detailed overview and comparison of the most widely used conjugation strategies in function of the active groups (carboxyl, amine, thiol, epoxy, hydroxyl and aldehyde groups) present on QD surface.

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Unique self-assembly properties of a bridge-shaped protein dimer with quantum dots

Cited by 20 publications

References 46 publications

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

Bioconjugation of quantum dots: Review & impact on future application

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