Unique stress response to the lactoperoxidase-thiocyanate enzyme system in Escherichia coli

Sermon, Jan; Vanoirbeek, Kristof; Spiegeleer, Philipp De; Houdt, Rob Van; Aertsen, Abram; Michiels, Chris W.

doi:10.1016/j.resmic.2004.09.012

Cited by 29 publications

(20 citation statements)

References 43 publications

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“…In addition, some genes that are inducible by H 2 O 2 (recA) or by O 2 Ϫ (sodA) were also induced by the LP/SCN Ϫ system. We concluded that the LP/SCN Ϫ system elicits a specific and unique stress response different from but partly overlapping with other oxidative stress responses (29). Similarly, Hansen et al (13) characterized the stress response in E. coli after challenge with the Curvularia haloperoxidase, using bromide as a substrate, and succeeded in establishing a link between one of the induced genes and bacterial tolerance to the haloperoxidase system.…”

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confidence: 71%

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CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Sermon

Wevers

Jansen

et al. 2005

Appl Environ Microbiol

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The enzyme lactoperoxidase is part of the innate immune system in vertebrates and owes its antimicrobial activity to the formation of oxidative reaction products from various substrates. In a previous study, we have reported that, with thiocyanate as a substrate, the lactoperoxidase system elicits a distinct stress response in Escherichia coli MG1655. This response is different from but partly overlapping with the stress responses to hydrogen peroxide and to superoxide. In the current work, we constructed knockouts in 10 lactoperoxidase system-inducible genes to investigate their role in the tolerance of E. coli MG1655 to this antimicrobial system. Five mutations resulted in a slightly increased sensitivity, but one mutation (corA) caused hypersensitivity to the lactoperoxidase system. This hypersensitive phenotype was specific to the lactoperoxidase system, since neither the sensitivity to hydrogen peroxide nor to the superoxide generator plumbagin was affected in the corA mutant. Salmonella enterica serovar Typhimurium corA had a similar phenotype. Although corA encodes an Mg 2؉ transporter and at least three other inducible open reading frames belonged to the Mg 2؉ regulon, repression of the Mg stimulon by Mg 2؉ did not change the lactoperoxidase sensitivity of either the wild-type or corA mutant. Prior exposure to 0.3 mM Ni 2؉ , which is also transported by CorA, strongly sensitized MG1655 but not the corA mutant to the lactoperoxidase system. Furthermore, this Ni 2؉ -dependent sensitization was suppressed by the CorA-specific inhibitor Co(III) hexaammine. These results indicate that CorA affects the lactoperoxidase sensitivity of E. coli by modulating the cytoplasmic concentrations of transition metals that enhance the toxicity of the lactoperoxidase system.

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confidence: 71%

“…In an earlier study we identified promoters specifically induced upon exposure to the antimicrobial LP/ SCN Ϫ system (29). The first objective of the present work was to evaluate the possible contribution of the corresponding genes in the protection of E. coli against the LP system.…”

Section: Construction and Lp Sensitivity Of Knockout Mutants In Lp-inmentioning

confidence: 96%

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Sermon

Wevers

Jansen

et al. 2005

Appl Environ Microbiol

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“…35) Lactoperoxidase also contributes to the antimicrobial defense in secretory fluids. 36,37) The hypothesis that salivary SCN − can protect the stomach from OH radicals formed by ascorbic acid/H 2 O 2 /Fe(II) systems under acidic conditions was reported. 38) Thus the change in SCN − concentration may have a significant effect not only on the oral cavity, but also on other sites in the body.…”

Section: Discussionmentioning

confidence: 99%

Effect of Psychologic Stress on Peroxidase and Thiocyanate Levels in Human Saliva Detected by Ultraweak Chemiluminescence

Goi

Takagi

Hirai

et al. 2007

JOURNAL OF HEALTH SCIENCE

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Saliva sampling has the advantage of being noninvasive and stress free. Based on a recent study, salivary ultraweak chemiluminescence (UCL) is a new biomarker of psychologic stress. However, it is not clear what causes changes in the UCL level and whether the change is biologically significant. We investigated the candidates for salivary UCL induced by psychological stressors and discuss the physiologic function of these candidates. Volunteers completed a questionnaire and then performed the Kraepelin test. Saliva was sampled just before, immediately after, and 30 min after the stress exposure. The UCL of saliva significantly increased just after stress exposure (1.56-fold) and returned to prestress levels after 30 min. The concentration of secretory immunoglobulin A also increased significantly and the change in both biomarkers was rapid. Similar significant changes were observed in salivary peroxidase activity and the concentration of thiocyanate (SCN − ). On the other hand, the levels of amylase activity did not significantly increase and the concentration of cortisol increased slowly. Moreover, in the reconstitution experiment, UCL was generated at the same level by a mixture of peroxidase and SCN − at physiologic concentrations. In conclusion, we determined that the Kraepelin test as a mental arithmetic task elicited a significant response in the body and this response can be calculated using salivary UCL. Furthermore, SCN − and peroxidase in the saliva play a key role in salivary UCL.

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“…The antimicrobial activity of LP is exerted through the oxidation of thiocyanate (SCN−) by hydrogen peroxide producing oxidation products as hypothiocyanite (OSCN−) (Carlsson et al, 1983; Le Nguyen et al, 2005) and hypthiocyanous acid (HOSCN), which show antimicrobial action (Sermon et al, 2005). Lactoperoxidase catalyzes the chemical reaction in milk of inorganic and organic substrates with the help of H2O2 (Al-Baarri et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

Arefin¹,

Sarker²,

Islam³

et al. 2017

J Adv Vet Anim Res

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Objective: Hydrogen peroxide (H2O2) was used for the activation of lactoperoxidase system on preservation of milk. Materials and methods: Milk samples were collected from Bangladesh Agricultural University dairy farm. The collected milk samples were added with 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% of 10% H2O2 along with control one. All milk samples were kept at room temperature (28-31 0 C). Flavor, clot on boiling (COB), acidity %, and methylene blue reduction (MBR) test were observed every one hour interval. Results: The milk samples were acceptable in terms of flavor up to 18 h at 0.14% H2O2. Similarly, this milk sample took maximum time (19 h) to give COB positive test. Acidity % was within normal range for 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% H2O2 treated milk samples up to 8,9,11,12,12,14,17 and 18 h, respectively. Significant difference (P<0.01) was found among the levels of H2O2 in acidity development at every observation hour except 0 hour. In MBR test, control sample was decolorized at 7 h and H2O2 treated milk samples were decolorized after 8 to 13 h depending on the level of added H2O2. Conclusion: Based on the results, it may be recommended that 0.14% H2O2 is suitable to extend the milk shelf-life where milk cooling facilities are not available.

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Unique stress response to the lactoperoxidase-thiocyanate enzyme system in Escherichia coli

Cited by 29 publications

References 43 publications

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Effect of Psychologic Stress on Peroxidase and Thiocyanate Levels in Human Saliva Detected by Ultraweak Chemiluminescence

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

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