Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix

Abstract

In this work, a universal chemiluminescence (CL) flow-through device suitable for various CL resonance energy transfer (CRET) systems has been successfully fabricated. Highly efficient CRET in solid-state photoactive organic molecules can be achieved by assembling them on the surface of layered double hydroxides (LDHs). We attribute these observations to the suppression of the intermolecular π-π stacking interactions among aromatic rings and the improvement of molecular orientation and planarity in the LDH matrix, enabling a remarkable increase in fluorescence lifetime and quantum yield of organic molecules. Under optimal conditions, using peroxynitrous acid-fluorescein dianion (FLUD) as a model CRET system, trace FLUD (10 μM) was assembled on the surface of LDHs. Peroxynitrous acid/nitrite could be assayed in the range of 1.0-500 μM, and the detection limit for peroxynitrous acid/nitrite (S/N = 3) was 0.6 μM. This CL flow-through device exhibited operational stability, high reproducibility, and long lifetime. While LDHs were immobilized in a flow-through device in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite was 100 μM. On the other hand, FLUD at the same concentration can not enhance the CL intensity of peroxynitrous acid system. This fabricated CL flow-through column has been successfully applied to determine nitrite in sausage samples with recoveries of 98-102%. These satisfactory results demonstrated that our studies pave a novel way toward flow-through column-based CRET using solid-state organic molecules as acceptors for signal amplification.

Gold Nanoclusters@Ru(bpy)32+-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe

“…As a result, the fluorescence lifetime and quantum yield of fluorescence dyes would increase when they were assembled on the surface of LDHs. 32,33 The successful improvements of FRET and CRET in the LDH matrix inspirits us to construct an efficient ERET process by layer-by-layer (LBL) electrostatic assembly of CoAl−LDH nanosheets with some ECL molecules (i.e., ERET donors and acceptors). However, the ERET with LDH nanosheets have not been established yet.…”

Single Step Synthesis of MoSe2−MoO3 Heterostructure for Highly Sensitive Amperometric Detection of Nitrite in Water Samples of Industrial Areas

“…Thus, the detrimental effect of nitrite on environment and human health makes its monitoring of utmost importance in public health and food industries. Although various analytical methods like spectrophotometry , chemiluminescence , capillary electrophoresis and chromatography have been developed for determination of nitrite, electrochemical methods gained wide attention due to their portability, low‐cost, specificity and sensitivity in real‐time analysis. In addition, electrochemical methods do not require any expensive instruments with time‐consuming and laborious procedures.…”

The determination of nitrite by a graphene quantum dot fluorescence quenching method without sample pretreatment

“…Spectrophotometry is another method in the National Standard for analysing nitrite, it is simple and suitable for batch sample analysis, but the method sensitivity needs improvement and the chromogenic reagent commonly used has a some toxicity. Chemiluminescence (CL) based on the microfluidic chip is also an effective way for the sensing nitrite, but this option is reagent‐ and time‐consuming when needed for large quantities of data. The other widely studied method is electrochemistry, which has stability and high accuracy, but the surface treatment of electrodes is somewhat time‐consuming and complicated.…”