Universal gold nanoparticle modified hybrid monolithic substrate developed for facile in-column post-functionalization

Online highly selective recognition of domoic acid by an aptamer@MOFs affinity monolithic column coupled with HPLC for shellfish safety monitoring

“…As shown in Table S2, † three batches of affinity monoliths were applied to calculate the aptamer coverage density, and the average value was gained as 3140 mmol L −1 , providing a high aptamer intensity on the monolith that was better than most reported previously. [27][28][29][30][31][32] The high coverage density of the aptamer on the resultant monolith enabled a favourable interface to enhance the specic recognition of DA in the complex samples.…”

“…[27][28][29] Using gold nanoparticles (AuNPs) as a medium, aptamer@AuNPs-functionalized monoliths with a high Brunauer-Emmett-Teller (BET) surface area and large content of aptamers have also been designed for perfect specic performance. [30][31][32] The selective IT-SPME of the massive amount of aptamers on the affinity monolith offers an attractive opportunity for the online selective capture and precise measurement of various analytes. 33 This sheds light on a promising approach for the online accurate recognition of DA.…”