Universal mini COI barcode for the identification of fish species in processed products

Sultana, Sharmin; Ali, Md. Eaqub; Hossain, M. A. Motalib; Asing,; Naquiah, Nina; Zaidul, I.S.M.

doi:10.1016/j.foodres.2017.10.065

Cited by 90 publications

(54 citation statements)

References 47 publications

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“…Primer yang digunakan yaitu primer universal Fish F1/R1 (Ward et al 2005) dan primer universal mini barcodes mini-F/R (Sultana et al 2018). Popa et al (2007) menyatakan bahwa kualitas produk PCR sangat tergantung pada kualitas isolat DNA.…”

Section: Hasil Amplifikasi Marka Molekuler Mini-barcode Coiunclassified

Mini-Coi Barcodes Sebagai Penanda Molekuler Untuk Ketertelusuran Label Pangan Berbagai Produk Olahan Ikan Sidat

et al. 2018

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Ikan Sidat merupakan komoditas hasil perikanan penting dengan nilai ekonomis tinggi. DNA barcoding telah cukup banyak digunakan sebagai metode berbasis penentuan urutan nukelotida untuk mendeteksi jenis spesies pada ikan. Proses pengolahan dengan suhu tinggi dapat menyulitkan proses identifikasi berbasis penentuan urutan nukelotida. Tujuan penelitian ini adalah untuk mengidentifikasi spesies berbagai produk olahan ikan sidat menggunakan marka molekuler mini-COI barcodes. Pengolahan suhu tinggi yang digunakan pada penelitian ini adalah: panggang, penggorengan dalam minyak sayur, panggang, pengasapan panas dan pengolahan suhu sterilisasi. Seluruh sampel segar dapat teridentifikasi sebagai spesies Anguilla bicolor bicolor (99-100%) dan Anguilla marmorata (100%). Sampel olahan sidat berhasil diamplifikasi dengan marka mini-COI barcodes, sehingga dapat digunakan untuk aplikasi pengawasan label produk seafood secara rutin.

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Section: Hasil Amplifikasi Marka Molekuler Mini-barcode Coiunclassified

Mini-Coi Barcodes Sebagai Penanda Molekuler Untuk Ketertelusuran Label Pangan Berbagai Produk Olahan Ikan Sidat

et al. 2018

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“…Parameter ini merupakan nilai yang digunakan dalam menjamin hasil PCR yang baik pada proses amplifikasi. Nilai kemurnian yang tidak berada pada angka tersebut diduga merupakan isolat dengan kontaminan yang Table 2 Amplification conditions for all primers in the study of DNA barcoding application for seafood label traceability of various commercial surimi-based products PCR Steps Primers F1R1/F2R2 (Ward et al 2005) Mini-barcode (Sultana et al 2017) Lago F/R (designed in this research) berupa lipid, protein, polisakarida, serta senyawa anorganik dan organik. Kontaminan tersebut dapat mengurangi kualitas DNA dan mengganggu keberhasilan analisis pada tahap selanjutnya (Muhammad et al 2016).…”

Section: Hasil Dan Pembahasan Konsentrasi Dan Kemurnian Isolat Dna Prunclassified

DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products

et al. 2019

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Surimi-based processed products are prone to mislabeling using raw materials that are not in accordance with food safety requirements. There were cases reporting use of toxic fish tissue in commercial seafood products. This study was aimed to identify and determine the raw materials used in various processed surimi products using cytochrome oxidase I (COI) gene marker. The experiment consisted of DNA isolation, DNA amplification using several target primers namely full-length barcodes, mini-barcodes, as well as specific species primers against poisonous puffer fish Lagocephalus lunaris and genetic analysis. The results of bioinformatics analysis revealed that S1 samples were Coryphaena hippurus or mahi-mahi fish, S2 and S3 samples were Nemipterus bathybius or curated fish and CS samples were Evynnis cardinalis or kuro fish. Detection of samples with species specific primers of puffer fish Lagocephalus lunaris with annealing temperatures of 54°C, 57°C, and 60°C showed no DNA bands in the six samples analyzed.

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“…Fish species authentication in the marketplace has become an international concern, mainly due to the widely reported species adulteration, where expensive or high‐demand species is replaced by a cheaper or more readily available one (Miller & Mariani, ; Ferrito et al , ; Xiong et al , ; Kim et al , ; Sultana et al , ; Xiong et al , ; Zeng et al , ; Sánchez et al , ). In particular, substitution of fish species in processed fish products is substantial, with an average prevalence rate of 30% in a recent investigation (ranging from less than 5% to 100%) (Pardo et al , ).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, several DNA amplification methods have been developed over the last years, including polymerase chain reaction (PCR), loop‐mediated isothermal amplification (LAMP) (Saull et al , ) and denaturation bubble‐mediated strand exchange amplification method (SEA) (Liu et al , ). Among these, PCR is the most widely used, and a wide variety of diagnostic methods have been raised, such as DNA barcoding, real‐time PCR and multiplex PCR (Hebert et al , ; Hajibabaei et al , ; Rasmussen & Morrissey, ; Herrero et al , ; Pappalardo & Ferrito, ; Cardenosa et al , ; Kim et al , ; Sultana et al , ). Particularly, the most straightforward approach consists in real‐time PCR which can amplify the target DNA with specificity, efficiency and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

Xiong

Yuan

Huang

et al. 2019

Int J of Food Sci Tech

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Summary Using species outside the order Gadiformes to prepare codfish products has been widely revealed as a new type of fraud in China, highlighting the necessity to establish proper methods for codfish species identification. This work aimed to develop a new SYBR Green real‐time PCR assay for the detection of codfish ingredient (mainly the eight Gadiformes species, Gadus morhua, Gadus macrocephalus, Theragra chalcogramma, Pollachius virens, Melanogrammus aeglefinus, Merluccius merluccius, Merluccius australis and Albatrossia pectoralis) in processed fish products based on mitochondrial 12S rRNA gene. Results highlighted the positive Cq (cycle quantification value, average value 19.18 ± 1.49) only from the eight Gadiformes species, with no fluorescent signal (Cq > 32) for the other sixty‐seven non‐Gadiformes species. The absolute limit of detection was 0.048 ng genomic DNA. Methodology validation succeeded in twenty‐six commercial products. These results demonstrated that the newly developed method is suitable for the identification of codfish ingredient in processed fish products.

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Universal mini COI barcode for the identification of fish species in processed products

Cited by 90 publications

References 47 publications

Mini-Coi Barcodes Sebagai Penanda Molekuler Untuk Ketertelusuran Label Pangan Berbagai Produk Olahan Ikan Sidat

Mini-Coi Barcodes Sebagai Penanda Molekuler Untuk Ketertelusuran Label Pangan Berbagai Produk Olahan Ikan Sidat

DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products

Development of a rapid method for codfish identification in processed fish products based on SYBR Green real‐time PCR

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