Universal probe library assay for the detection of infectious pancreatic necrosis virus genogroups 1 and 5 in salmonid tissues

Avendaño‐Herrera, Rubén; Tobar, Jaime A.; Tobar, Ivan Felipe Gutiérrez; Fernández-Caro, Claudia

doi:10.1111/are.12945

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…Each group was composed of nine individuals (three individuals by each replicate), resulting in one thermal profile for each group (see Figures 3 A–C). Viral load was quantified using RT-qPCR assays for the genome segment WP2 of the IPNv ( 39 , 40 , 55 ). Viral loads were higher in individuals that have not developed behavioral fever, in contrast to the fever group (Figure 1 B).…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA used for high-throughput transcriptome sequencing, RT-qPCR and flow cytometry analysis, was previously analyzed in order to attest and confirmed the successful completion of infection with (IPNv). To do so, an RT-PCR was performed for IPNv load estimation by targeting the virus segment VP2 region using primer WB117 and a Universal ProbeLibrary probes as previously described ( 38 – 40 ).…”

Section: Methodsmentioning

confidence: 99%

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Behavioral Fever Drives Epigenetic Modulation of the Immune Response in Fish

et al. 2018

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Ectotherms choose the best thermal conditions to mount a successful immune response, a phenomenon known as behavioral fever. The cumulative evidence suggests that behavioral fever impacts positively upon lymphocyte proliferation, inflammatory cytokine expression, and other immune functions. In this study, we have explored how thermal choice during infection impacts upon underpinning molecular processes and how temperature increase is coupled to the immune response. Our results show that behavioral fever results in a widespread, plastic imprint on gene regulation, and lymphocyte proliferation. We further explored the possible contribution of histone modification and identified global associations between temperature and histone changes that suggest epigenetic remodeling as a result of behavioral fever. Together, these results highlight the critical importance of thermal choice in mobile ectotherms, particularly in response to an infection, and demonstrate the key role of epigenetic modification to orchestrate the thermocoupling of the immune response during behavioral fever.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Behavioral Fever Drives Epigenetic Modulation of the Immune Response in Fish

et al. 2018

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“…In fact, real‐time RT‐PCR with universal probe library assay probes has high potential to be a potent tool for early IPNv diagnosis for Chilean genogroups 1 and 5 in salmonid tissues (Avendaño‐Herrera et al . ). These techniques require less time to determine any viral presence.…”

Section: Infectious Pancreatic Necrosis Virusmentioning

confidence: 97%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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“…; Bastardo, Ravelo & Romalde ; Avendaño‐Herrera et al . ). The samples tested positive only for Y. ruckeri , with Ct values of 22.2 ± 0.8 and 18.33 ± 0.5 for the Bio‐Bio and Araucania farms, respectively.…”

Section: Biochemical Characterization Of the Chilean Coho Salmon Isolmentioning

confidence: 97%

Disease caused by Yersinia ruckeri serotype O2b found in Chilean‐farmed coho salmon, Oncorhynchus kisutch (Walbaum, 1792)

Avendaño‐Herrera

Tapia‐Cammas

Aedo³

et al. 2016

Journal of Fish Diseases

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Universal probe library assay for the detection of infectious pancreatic necrosis virus genogroups 1 and 5 in salmonid tissues

Cited by 6 publications

References 22 publications

Behavioral Fever Drives Epigenetic Modulation of the Immune Response in Fish

Behavioral Fever Drives Epigenetic Modulation of the Immune Response in Fish

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Disease caused by Yersinia ruckeri serotype O2b found in Chilean‐farmed coho salmon, Oncorhynchus kisutch (Walbaum, 1792)

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