Untargeted and targeted metabolomics identify metabolite biomarkers for Salmonella enteritidis in chicken meat

Chen, Lan; Ding, Hao; Zhu, Yali; Guo, Yawen; Tang, Yayun; Xie, Kaizhou; Zhang, Genxi; Dai, Guojun; YuShi, Gao; Zhang, Tao

doi:10.1016/j.foodchem.2022.135294

Cited by 25 publications

(10 citation statements)

References 41 publications

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“…The R2Y and Q 2 values of the PLS‐DA models were 0.808 and 0.726, respectively. Figure 3d presents the validation results of 200 random permutation tests, which generated intercepts with Q 2 = ‐0.641, indicating the model's reliability without overfitting (Chen et al., 2023 ; Li et al., 2019 ). The loading scatter plots of PLS‐DA revealed that valine, glucose, isoleucine, leucine, 3‐methyl‐2‐oxovalerate, 2‐oxoisocaproate, tryptophan, 3‐hydroxybutyrate, and O‐acetylcarnitine were the main contributors to the separation between groups (Figure 3e ).…”

Section: Resultsmentioning

confidence: 99%

¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

Chen,

Wang,

Guo

et al. 2024

Food Science & Nutrition

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The primary objective of this investigation was to explore the beneficial impacts of Enteromorpha prolifera polysaccharide (EP) on dysglycemia in Zucker diabetic fatty (ZDF) rats, while also shedding light on its potential mechanism using 1H‐NMR‐based metabolomics. The results demonstrated a noteworthy reduction in fasting blood glucose (FBG, 46.3%), fasting insulin (50.17%), glycosylated hemoglobin A1c (HbA1c, 44.1%), and homeostatic model assessment of insulin resistance (HOMA‐IR, 59.75%) following EP administration, while the insulin sensitivity index (ISI, 19.6%) and homeostatic model assessment of β‐cell function (HOMA‐β, 2.5‐fold) were significantly increased. These findings indicate that EP enhances β‐cell function, increases insulin sensitivity, and improves insulin resistance caused by diabetes. Moreover, EP significantly reduced serum lipid levels, suggesting improvement of dyslipidemia. Through the analysis of serum metabolomics, 17 metabolites were found to be altered in diabetic rats, 14 of which were upregulated and 3 of which were downregulated. Notably, the administration of EP successfully reversed the abnormal levels of 9 out of the 17 metabolites. Pathway analysis further revealed that EP treatment partially restored metabolic dysfunction, with notable effects observed in valine, leucine, and isoleucine metabolism; aminoacyl‐transfer RNA (tRNA) biosynthesis; and ketone body metabolism. These findings collectively indicate the potential therapeutic efficacy of EP in preventing glycemic abnormalities and improving insulin resistance. Thus, EP holds promise as a valuable treatment option for individuals with diabetes.

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Section: Resultsmentioning

confidence: 99%

¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

Chen,

Wang,

Guo

et al. 2024

Food Science & Nutrition

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“…The cut-off range for candidate selection was as follows: variable importance in the projection (VIP) value >1, an absolute p -value >0.05 ( Chhonker et al ., 2021 ; Shi et al ., 2021 ), an absolute p (correlation) value ≥0.4, and an endogenous metabolite. To assess the risk of model overfitting, the 200-times permutation test was performed ( Zhu et al ., 2020 ; Chen et al ., 2023 ). For candidate metabolites selected through multivariate analysis, validation was conducted by confirming their consistency through compound identification.…”

Section: Methodsmentioning

confidence: 99%

Metabolomic Profiles in Patients with Cervical Cancer Undergoing Cisplatin and Radiation Therapy

Choi,

Kim,

Jeon

et al. 2024

Biomol Ther (Seoul)

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This study was aimed to evaluate endogenous metabolic changes before and after cisplatin and radiation therapy in patients with cervical cancer via untargeted metabolomic analysis using plasma samples. A total of 13 cervical cancer patients were enrolled in this study. Plasma samples were collected from each patient on two occasions: approximately one week before therapy (P1) and after completion of cisplatin and radiation therapy (P2). Of the 13 patients, 12 patients received both cisplatin and radiation therapy, whereas one patient received radiation therapy alone. The samples were analyzed using the Ultimate 3000 coupled with Q Exactive TM Focus Hybrid Quadrupole-Orbitrap TM mass spectrometry (Thermo Fisher Scientific, Waltham, MA, USA). Chromatographic separation utilized a Kinetex C18 column 2.1×100 mm (2.6 μm) (Phenomenex, Torrance, CA, USA), and the temperature was maintained at 40°C. Following P2, there were statistically significant increases in the concentrations of indoxyl sulfate, phenylacetylglutamine, Lysophosphatidyethanolamine (LysoPE) (18:1), and indole-3-acetic acid compared with the concentrations observed at P1. Specifically, in the human papillomavirus (HPV) noninfection group, indoxyl sulfate, LysoPE (18:1), and phenylacetylglutamine showed statistically significant increases at P2 compared with P1. No significant changes in metabolite concentrations were observed in the HPV infection group. Indoxyl sulfate, LysoPE (18:1), phenylacetylglutamine, and indole-3-acetic acid were significantly increased following cisplatin and radiation therapy.

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“…Samples were sent to Shanghai Biotree Biomedical Technology Co., Ltd. for analysis. Targeted metabolomics analysis was performed by the ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS) and was provided by BIOTREE (Shanghai, China) [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. The model validity was evaluated by 7-fold cross-validation, and a permutation test ( n = 200) VIP > 1 and P < 0.05 were used as screening thresholds for differentially abundant metabolites [ 37 ]. Statistical significance was defined as p < 0.05.…”

Section: Methodsmentioning

confidence: 99%

Prevotella copri transplantation promotes neurorehabilitation in a mouse model of traumatic brain injury

Gu,

Yan,

Tang

et al. 2024

J Neuroinflammation

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Background The gut microbiota plays a critical role in regulating brain function through the microbiome-gut-brain axis (MGBA). Dysbiosis of the gut microbiota is associated with neurological impairment in Traumatic brain injury (TBI) patients. Our previous study found that TBI results in a decrease in the abundance of Prevotella copri (P. copri). P. copri has been shown to have antioxidant effects in various diseases. Meanwhile, guanosine (GUO) is a metabolite of intestinal microbiota that can alleviate oxidative stress after TBI by activating the PI3K/Akt pathway. In this study, we investigated the effect of P. copri transplantation on TBI and its relationship with GUO-PI3K/Akt pathway. Methods In this study, a controlled cortical impact (CCI) model was used to induce TBI in adult male C57BL/6J mice. Subsequently, P. copri was transplanted by intragastric gavage for 7 consecutive days. To investigate the effect of the GUO-PI3K/Akt pathway in P. copri transplantation therapy, guanosine (GUO) was administered 2 h after TBI for 7 consecutive days, and PI3K inhibitor (LY294002) was administered 30 min before TBI. Various techniques were used to assess the effects of these interventions, including quantitative PCR, neurological behavior tests, metabolite analysis, ELISA, Western blot analysis, immunofluorescence, Evans blue assays, transmission electron microscopy, FITC-dextran permeability assay, gastrointestinal transit assessment, and 16 S rDNA sequencing. Results P. copri abundance was significantly reduced after TBI. P. copri transplantation alleviated motor and cognitive deficits tested by the NSS, Morris’s water maze and open field test. P. copri transplantation attenuated oxidative stress and blood-brain barrier damage and reduced neuronal apoptosis after TBI. In addition, P. copri transplantation resulted in the reshaping of the intestinal flora, improved gastrointestinal motility and intestinal permeability. Metabolomics and ELISA analysis revealed a significant increase in GUO levels in feces, serum and injured brain after P. copri transplantation. Furthermore, the expression of p-PI3K and p-Akt was found to be increased after P. copri transplantation and GUO treatment. Notably, PI3K inhibitor LY294002 treatment attenuated the observed improvements. Conclusions We demonstrate for the first time that P. copri transplantation can improve GI functions and alter gut microbiota dysbiosis after TBI. Additionally, P. copri transplantation can ameliorate neurological deficits, possibly via the GUO-PI3K/Akt signaling pathway after TBI.

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Untargeted and targeted metabolomics identify metabolite biomarkers for Salmonella enteritidis in chicken meat

Cited by 25 publications

References 41 publications

¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

Metabolomic Profiles in Patients with Cervical Cancer Undergoing Cisplatin and Radiation Therapy

Prevotella copri transplantation promotes neurorehabilitation in a mouse model of traumatic brain injury

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Untargeted and targeted metabolomics identify metabolite biomarkers for Salmonella enteritidis in chicken meat

Cited by 25 publications

References 41 publications

1H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

1H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

Metabolomic Profiles in Patients with Cervical Cancer Undergoing Cisplatin and Radiation Therapy

Prevotella copri transplantation promotes neurorehabilitation in a mouse model of traumatic brain injury

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¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats

¹H‐NMR‐based metabolomics reveals the preventive effect of Enteromorpha prolifera polysaccharides on diabetes in Zucker diabetic fatty rats