Unusual base pairing during the decoding of a stop codon by the ribosome

Fernandez, I.S.; Ng, Chyan Leong; Kelley, A.C.; Wu, Guowei; Yu, Yi; Ramakrishnan, V.

doi:10.1038/nature12302

Cited by 146 publications

(145 citation statements)

References 19 publications

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“…Similarly, C was found to be widely present in mRNA and change in response to environmental signals, including starvation and heat shock in yeast and serum starvation in human cells [43][44][45]. How these modifications influence mRNP biogenesis and the gene expression program at large remains to be seen, but it is interesting to consider RNA modification as another means of altering the genetic code, the effect of which can be dramatic given the reported ability of C to alter decoding of stop codons [49,50].…”

Section: A Role For Rna Modificationmentioning

confidence: 96%

Emerging properties of nuclear RNP biogenesis and export

Oeffinger¹,

Montpetit²

2015

Current Opinion in Cell Biology

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Section: A Role For Rna Modificationmentioning

confidence: 96%

Emerging properties of nuclear RNP biogenesis and export

Oeffinger¹,

Montpetit²

2015

Current Opinion in Cell Biology

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“…Artificially targeted pseudouridylation of stop codons in vivo leads to noncanonical base pairing of Ψ in the decoding center of the ribosome and efficient nonsense suppression (Karijolich & Yu, 2011). Structural studies of this surprising phenomenon suggested the possibility of more widespread effects of pseudouridine on decoding (Fernández et al, 2013).…”

Section: Rna Modificationsmentioning

confidence: 97%

Pseudo-Seq

Carlile

Rojas-Durán

Gilbert

2015

Methods in Enzymology

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RNA molecules contain a variety of chemically diverse, posttranscriptionally modified bases. The most abundant modified base found in cellular RNAs, pseudouridine (Ψ), has recently been mapped to hundreds of sites in mRNAs, many of which are dynamically regulated. Though the pseudouridine landscape has been determined in only a few cell types and growth conditions, the enzymes responsible for mRNA pseudouridylation are universally conserved, suggesting many novel pseudouridylated sites remain to be discovered. Here, we present Pseudo-seq, a technique that allows the identification of sites of pseudouridylation genome-wide with single-nucleotide resolution. In this chapter, we provide a detailed description of Pseudo-seq. We include protocols for RNA isolation from Saccharomyces cerevisiae, Pseudo-seq library preparation, and data analysis, including descriptions of processing and mapping of sequencing reads, computational identification of sites of pseudouridylation, and assignment of sites to specific pseudouridine synthases. The approach presented here is readily adaptable to any cell or tissue type from which high-quality mRNA can be isolated. Identification of novel pseudouridylation sites is an important first step in elucidating the regulation and functions of these modifications.

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“…Em condições normais, este processo ocorre com três códons de parada diferentes, UGA, UAA ou UAG (Fernández et al, 2013).…”

Section: Mecanismos Envolvidos Na Síntese Proteicaunclassified

Síntese proteica em suínos: como fêmeas, machos não castrados e castrados respondem a este processo?

Monteiro

Huepa

Castilha

et al. 2017

Pubvet

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RESUMO.A síntese proteica muscular em suínos é essencial para promover o ganho de carne marga. Entretanto, é um processo complexo que envolve inúmeras enzimas, proteínas ribossomais e pode ser influenciado por diversos fatores, de ordem ambiental, nutricional e hormonal. Neste sentido, os hormônios sexuais podem exercer ação anabólica na síntese proteica, levando a diferentes taxas de deposição muscular em machos castrados, machos não castrados e fêmeas. Com o intuito de aproveitar o efeito anabólico da testosterona, a imunocastração vem sendo cada vez mais difundida na suinocultura, levando machos imunocastrados a apresentarem desempenho e características de carcaça semelhantes a machos inteiros. De forma geral, o sexo parece desempenhar um importante papel na síntese proteica. Isso confere diferentes exigências de aminoácidos, em machos inteiros, fêmeas e machos castrados, devido às diferenças na deposição de proteína na carcaça.Palavras chave: fêmeas, imunocastração, machos, síntese muscular Protein synthesis in pigs: how gilts, barrows and intact male respond to this process?ABSTRACT. Muscle protein synthesis in pigs can be considered a key factor in promoting lean gain. However, this is a complex process depending on several enzymes, ribosomal proteins and may be affected by environmental factors, besides nutritional and hormonal. In this sense, sexual hormones may have anabolic action in protein synthesis, leading different rates of muscle deposition in barrows, intact males and gilts. Aiming to use the anabolic effect of testosterone, the immunocastration practice is being applied to pig production in many countries, allowing males to show the same growth performance and carcass traits as intact males. In this sense, sex plays an important role on protein synthesis, providing differences in the amino acids requirements, according to its condition (intact males, gilts and barrows) due to differences in carcass protein deposition.Keywords: Gilts, immunocastration, barrows, muscle synthesis Síntesis proteica en porcinos: como las hembras, los machos castrados y los machos enteros responden a este proceso?RESUMEN. La síntesis de proteína muscular en porcinos es esencial para estimular la ganancia de carne magra. Sin embargo es un proceso complejo que involucra una cantidad innumerable de enzimas, proteínas ribosomales y puede ser influenciada por diferentes factores de orden ambiental, nutricional y hormonal. En este sentido, las hormonas sexuales pueden ejercer una acción anabólica en la síntesis de proteínas, conllevando a diferentes

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Unusual base pairing during the decoding of a stop codon by the ribosome

Cited by 146 publications

References 19 publications

Emerging properties of nuclear RNP biogenesis and export

Emerging properties of nuclear RNP biogenesis and export

Pseudo-Seq

Síntese proteica em suínos: como fêmeas, machos não castrados e castrados respondem a este processo?

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