Unusual polyphosphate inclusions observed in a marine Beggiatoa strain

Brock, Jörg; Rhiel, Erhard; Beutler, Martin; Salman, Verena; Schulz-Vogt, Heide N.

doi:10.1007/s10482-011-9640-8

Cited by 29 publications

(31 citation statements)

References 49 publications

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“…It is possible that the polyphosphate bodies are surrounded with a membranous cover to which DNA fibres are attached; proteins that bind both DNA and membranes are known in chloroplasts (Jeong et al 2003;Kabeya et al 2010). There have been several reports indicating the existence of membranes surrounding polyphosphate bodies or volutin granules, which are now known as acidocalcisomes, and similarities between vacuoles and these structures have been pointed out (Seufferheld et al 2003(Seufferheld et al , 2011Brock et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Observation of polyphosphate bodies and DNA during the cell division cycle of Synechococcus elongatus PCC 7942

2013

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Although most cyanobacterial cells contain prominent polyphosphate bodies in the central cytoplasmic area enclosed by the peripheral thylakoid membranes, their roles are not fully understood. Storing phosphate for nucleotide production might be one of their important roles in survival of the cells. As a step towards identifying a possible contribution of the polyphosphate bodies to DNA synthesis, the relationship between polyphosphate bodies and DNA throughout cell division cycle of Synechococcus elongatus PCC 7942 cells cultured under light/dark cycles was investigated with light and electron microscopy. During the dark period, the average size of polyphosphate bodies increased gradually without significant change in their number and distribution. However, during the light period, the number of polyphosphate bodies increased, while the size of each polyphosphate body decreased and cells elongated until the end of the light period, when most cells divided. The ratio of the content of polyphosphate bodies to cell length increased gradually during the dark period and decreased during the light period. Hoechst 33342-stained DNA appeared diffuse during the dark period, but in the light period it became condensed and eventually formed a wavy, rope-like structure prior to cell division. Close association between fibres containing DNA and polyphosphate bodies was demonstrated by TEM using DNA-specific staining and BrdU labelling. These regular coordinated changes of polyphosphate bodies and DNA shape during the cell division cycle, together with their intimate interaction, imply a role of polyphosphate bodies in supplying material for DNA.

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Section: Discussionmentioning

confidence: 99%

Observation of polyphosphate bodies and DNA during the cell division cycle of Synechococcus elongatus PCC 7942

2013

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“…The marine sulfide-oxidizing bacteria Beggiatoa were reported to accumulate polyP together with cations in well-delimited granules enclosed by a lipid layer with similarity to acidocalcisomes, although not particularly acidic [34]. Similar polyP and cation storage structures (small granules) were found in the Gram-negative sporulating bacterium Acetonema longum [35].…”

Section: Origin Of the Acidocalcisomementioning

confidence: 99%

The origin and evolution of the acidocalcisome and its interactions with other organelles

Docampo

2016

Molecular and Biochemical Parasitology

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Acidocalcisomes are acidic calcium stores that have been found from bacteria to human cells. They are rich in phosphorus compounds in the form of orthophosphate (Pi), pyrophosphate (PPi), and polyphosphate (polyP) and their acidity is maintained by proton pumps such as the vacuolar proton pyrophosphatase (V-H+-PPase, or VP1), the vacuolar proton ATPase (V-H+-ATPase), or both. Recent studies in trypanosomatids and in other species have revealed their role in phosphate metabolism, and cation and water homeostasis, as suggested by the presence of novel pumps, transporters, and channels. An important role in autophagy has also been described. The study of the biogenesis of acidocalcisomes as well as of the interactions of these lysosome-related organelles with other organelles have uncovered important roles in calcium signaling and osmoregulation. Significantly, despite conservation of acidocalcisomes across all of cellular life, there is evidence for intimate integration of these organelles with eukaryotic cellular functions, and which are directly relevant to parasites.

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“…Accumulation of phosphorus in the form of polyphosphate has been reported for some bacteria of the family Beggiatoaceae (Schulz and Schulz, 2005;Brock and Schulz-Vogt, 2011;Brock et al, 2012). In previous studies, T. ingrica samples were subjected to Toluidine blue staining to visualize intracellular polyphosphate granules, but only negative results were obtained (Høgslund et al, 2010;Nemoto et al, 2011).…”

Section: Phosphorus Metabolismmentioning

confidence: 99%

Ecophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence

et al. 2014

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Large sulfur-oxidizing bacteria, which accumulate a high concentration of nitrate, are important constituents of aquatic sediment ecosystems. No representative of this group has been isolated in pure culture, and only fragmented draft genome sequences are available for these microorganisms. In this study, we successfully reconstituted the genome of Thioploca ingrica from metagenomic sequences, thereby generating the first complete genome sequence from this group. The Thioploca samples for the metagenomic analysis were obtained from a freshwater lake in Japan. A PCR-free paired-end library was constructed from the DNA extracted from the samples and was sequenced on the Illumina MiSeq platform. By closing gaps within and between the scaffolds, we obtained a circular chromosome and a plasmid-like element. The reconstituted chromosome was 4.8 Mbp in length with a 41.2% GC content. A sulfur oxidation pathway identical to that suggested for the closest relatives of Thioploca was deduced from the reconstituted genome. A full set of genes required for respiratory nitrate reduction to dinitrogen gas was also identified. We further performed a proteomic analysis of the Thioploca sample and detected many enzymes/proteins involved in sulfur oxidation, nitrate respiration and inorganic carbon fixation as major components of the protein extracts from the sample, suggesting that these metabolic activities are strongly associated with the physiology of T. ingrica in lake sediment.

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Unusual polyphosphate inclusions observed in a marine Beggiatoa strain

Cited by 29 publications

References 49 publications

Observation of polyphosphate bodies and DNA during the cell division cycle of Synechococcus elongatus PCC 7942

Observation of polyphosphate bodies and DNA during the cell division cycle of Synechococcus elongatus PCC 7942

The origin and evolution of the acidocalcisome and its interactions with other organelles

Ecophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence

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