Unveiling the mode of action of antibacterial labdane diterpenes from Alpinia nigra (Gaertn.) B. L. Burtt seeds

Ghosh, Sudhamoy; Indukuri, Kiran; Bondalapati, Somasekhar; Saikia, Anil K.; Rangan, Latha

doi:10.1016/j.ejmech.2013.05.034

Cited by 42 publications

(30 citation statements)

References 21 publications

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“…Ghosh and colleagues found by measuring of the extent of propidium iodide DNA staining of treated cells that both compounds caused significant increase in fluorescence intensity compared to the controls, which indicates membrane damage. The interpretation of this finding was supported by field emission scanning electronic microscopy; they observed membrane disintegration and significant damage to the cell wall [57]. …”

Section: Application Of Flow Cytometrymentioning

confidence: 93%

“…Additionally, the use of these dyes has the advantage that no pretreatments of cells were required. Other studies have succeeded in employing fluorescent probes to measure the efficiency of new antibacterial drugs [12, 56, 57]. For instance, Ghosh et al [57] demonstrated by FCM the antibacterial activity of two bioactive compounds isolated from seeds of Alpinia nigra against seven pathogenic bacteria.…”

Section: Application Of Flow Cytometrymentioning

confidence: 99%

“…Other studies have succeeded in employing fluorescent probes to measure the efficiency of new antibacterial drugs [12, 56, 57]. For instance, Ghosh et al [57] demonstrated by FCM the antibacterial activity of two bioactive compounds isolated from seeds of Alpinia nigra against seven pathogenic bacteria. Ghosh and colleagues found by measuring of the extent of propidium iodide DNA staining of treated cells that both compounds caused significant increase in fluorescence intensity compared to the controls, which indicates membrane damage.…”

Section: Application Of Flow Cytometrymentioning

confidence: 99%

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Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Ambriz-Aviña

Contreras‐Garduño

Pedraza-Reyes

2014

BioMed Research International

125

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Although reports of flow cytometry (FCM) applied to bacterial analysis are increasing, studies of FCM related to human cells still vastly outnumber other reports. However, current advances in FCM combined with a new generation of cellular reporter probes have made this technique suitable for analyzing physiological responses in bacteria. We review how FCM has been applied to characterize distinct physiological conditions in bacteria including responses to antibiotics and other cytotoxic chemicals and physical factors, pathogen-host interactions, cell differentiation during biofilm formation, and the mechanisms governing development pathways such as sporulation. Since FCM is suitable for performing studies at the single-cell level, we describe how this powerful technique has yielded invaluable information about the heterogeneous distribution of differently and even specialized responding cells and how it may help to provide insights about how cell interaction takes place in complex structures, such as those that prevail in bacterial biofilms.

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Section: Application Of Flow Cytometrymentioning

confidence: 93%

Section: Application Of Flow Cytometrymentioning

confidence: 99%

Section: Application Of Flow Cytometrymentioning

confidence: 99%

See 1 more Smart Citation

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Ambriz-Aviña

Contreras‐Garduño

Pedraza-Reyes

2014

BioMed Research International

125

View full text Add to dashboard Cite

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“…The MIC was determined using the microdilution method, in 96 wells microtitre plates according to protocol described by Ghosh et al (2013). Compounds were dissolved in dimethyl sulfoxide (DMSO) at concentration of 2 mg/mL and this concentration was further used to make compound with concentration up to 1.95 µg/mL by two fold dilution method.…”

Section: Minimum Inhibitory Concentration (Mic) Is the Lowest Concentmentioning

confidence: 99%

Synthesis, In silico studies and In vitro evaluation for antioxidant and antibacterial properties of diarylmethylamines: A novel class of structurally simple and highly potent pharmacophore

Mahato

Singh

Rangan

et al. 2016

European Journal of Pharmaceutical Sciences

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“…In addition, the mode of action of two labdane diterpenes, ( E )-labda-8(17),12-diene-15,16-dial ( 6 ) and ( E )-8 β ,17-epoxylabd-12-ene-15,16-dial ( 7 ) (Figure 2), was recently investigated. The results revealed that these molecules caused significant damage and disintegration to the bacterial cell membranes and cell leakage was found [37]. …”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of Coronarin D and Its Synergistic Potential with Antibiotics

Reuk-Ngam

Chimnoi

Khunnawutmanotham

et al. 2014

BioMed Research International

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Coronarin D is a labdane-type diterpene from the rhizomes of Hedychium coronarium. In the view of our ongoing effort to explore its novel biological activity, antimicrobial activity study of coronarin D was performed. The results showed that coronarin D was active against tested Gram-positive bacteria, inactive for tested Gram-negative bacteria, and weakly active against tested fungi. The antibacterial effect of the combination of coronarin D with nine classical antibiotics against four Gram-positive bacteria was also evaluated. The fractional inhibitory concentration indices (FICI) of coronarin D-antibiotics combinations, calculated from the checkerboard assay, were used as synergism indicator. Out of 36 combinations, 47% showed total synergism, 33% had partial synergistic interaction, 17% showed no effect, and 3% showed antagonism. By combination with coronarin D at concentration of 0.25 minimal inhibitory concentration (MIC), the activities of antibiotics were boosted to 4- to 128-fold. These finding suggested an attractive approach to combat the infectious diseases by using coronarin D-antibiotic drug combination.

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Unveiling the mode of action of antibacterial labdane diterpenes from Alpinia nigra (Gaertn.) B. L. Burtt seeds

Cited by 42 publications

References 21 publications

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Applications of Flow Cytometry to Characterize Bacterial Physiological Responses

Synthesis, In silico studies and In vitro evaluation for antioxidant and antibacterial properties of diarylmethylamines: A novel class of structurally simple and highly potent pharmacophore

Antimicrobial Activity of Coronarin D and Its Synergistic Potential with Antibiotics

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