Unveiling the role of long-range and short-range forces in the non-productive adsorption between lignin and cellulases at different temperatures

Xu, Li; Wang, Jingyu; Zhang, Aiting; Pang, Yongqiang; Yang, Dongjie; Li, Hongming; Qiu, Xueqing

doi:10.1016/j.jcis.2023.05.152

Cited by 10 publications

(4 citation statements)

References 41 publications

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“…To characterize the successful modification of enzymes on AFM probe tips, the morphology of the AFM probes was examined using field emission scanning electron microscopy (FE-SEM, Regulus 8100, Hitachi), and the elements C, N, and O on the AFM probe tips were scanned using energy-dispersive spectroscopy (EDS). 40,41 The FE-SEM scanning parameters were set to an acceleration voltage of 3.0 kV and a magnification of 20000×.…”

Section: Characterization Of Sl and Rlmentioning

confidence: 99%

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Elucidating the nonproductive adsorption mechanism of cellulase with lignin fractions from hydrothermally pretreated poplar using multi-dimensional spectroscopic technologies

et al. 2023

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Section: Characterization Of Sl and Rlmentioning

confidence: 99%

“…S2 †). 40,41 In addition, the elements of C, O, and N on the probe were characterized using the SEM-EDS module. From Fig.…”

Section: Quantitative Characterization Of the Interaction Force Betwe...mentioning

confidence: 99%

Elucidating the nonproductive adsorption mechanism of cellulase with lignin fractions from hydrothermally pretreated poplar using multi-dimensional spectroscopic technologies

et al. 2023

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“…Notably, surfaces coated with polymers containing a higher cationic content exhibited smaller contact angles, indicating an enhanced hydrophilicity of the lignin surface. Our earlier work has revealed that hydrophobic interactions primarily drive nonproductive adsorption between lignin and cellulase . Accordingly, PAD-X is able to alleviate the nonproductive adsorption by improving the hydrophilicity of the lignin surface.…”

Section: Resultsmentioning

confidence: 99%

“…Our earlier work has revealed that hydrophobic interactions primarily drive nonproductive adsorption between lignin and cellulase. 40 Accordingly, PAD-X is able to alleviate the nonproductive adsorption by improving the hydrophilicity of the lignin surface.…”

Section: Effect Of Cationic Copolymers On the High-solid Enzymatic Hy...mentioning

confidence: 99%

Copolymer with Tunable Cation Content as an Efficient Promoter in Enzymatic Saccharification of Lignocellulose

Xu,

Zhang,

Liao

et al. 2023

ACS Appl. Polym. Mater.

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Nonproductive adsorption of cellulase is widely considered as the major roadblock to enzymatically hydrolyzing lignocellulosic biomass. Cationic additives present a promising avenue for mitigating the issue by effectively obstructing cellulase adsorption onto the lignin surface through enhanced electrostatic interactions with the negatively charged lignin, in contrast to nonionic additives. However, excessive positive charges carried by ionic additives can potentially hamper enzyme activity. To reconcile the dilemma, we synthesized a copolymer featuring modulatable cationic content via control over the ratio of nonionic and cationic units within the polymer backbone. Upon addition of 1.0 g/L of the resultant cationic copolymer, a notable enhancement in glucose release from 39 to 88% was observed, even at a low cellulase dosage of 5 FPU/g glucan. Remarkably, these cationic copolymers exhibit versatile applicability across diverse enzyme preparations, acid-treated lignocellulosic substrates, and highly solid saccharification systems. Furthermore, an enzyme saving of up to 80% was achieved. Mechanistic studies illuminated that this copolymer can not only attenuate the occurrence of irreversible binding between lignin and cellulase but also exert a favorable influence on enzyme activity. The adaptability of the copolymer’s cationic content endows it with the status of a promising and effective lignin-blocking additive, holding considerable potential for advancing the economic feasibility of biofuel production.

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Optimizing bioconversion processes of rice husk into value-added products: D-psicose, bioethanol, and lactic acid

Song,

Maskey,

Lee

et al. 2024

Bioresource Technology

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Unveiling the role of long-range and short-range forces in the non-productive adsorption between lignin and cellulases at different temperatures

Cited by 10 publications

References 41 publications

Elucidating the nonproductive adsorption mechanism of cellulase with lignin fractions from hydrothermally pretreated poplar using multi-dimensional spectroscopic technologies

Elucidating the nonproductive adsorption mechanism of cellulase with lignin fractions from hydrothermally pretreated poplar using multi-dimensional spectroscopic technologies

Copolymer with Tunable Cation Content as an Efficient Promoter in Enzymatic Saccharification of Lignocellulose

Optimizing bioconversion processes of rice husk into value-added products: D-psicose, bioethanol, and lactic acid

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