Up-regulated pathways in response to Deformed Wing Virus infection in Apis mellifera (Hymenoptera: Apidae)

Quintana, Silvina; Brasesco, Constanza; Negri, Pedro; MARIN, Maia; Pagnuco, Inti Anabela; Szawarski, Nicolás; Reynaldi, Francisco José; Larsen, Alejandra Edith; Eguaras, Martín Javier; Maggi, Matías

doi:10.25085/rsea.780101

Cited by 20 publications

(14 citation statements)

References 56 publications

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“…The cellular immune response can be measured based on the number of circulating hemocytes after challenging (Brandt et al 2016(Brandt et al , 2017Quintana et al 2019). Therefore, we measured the THC in M. quadrifasciata after exposure to azadirachtin and spinosad in uninfected bees and bees infected with E. coli .…”

Section: Total Hemocyte Countmentioning

confidence: 99%

Changes in innate immune response and detoxification in Melipona quadrifasciata (Apinae: Meliponini) on oral exposure to azadirachtin and spinosad

et al. 2020

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Exposure to agrochemicals and pathogens has been indicated as critical factors of declining pollinator populations, including species of native tropical bees. In the present study, we investigated the sublethal effects of acute oral exposure to azadirachtin and spinosad bioinsecticides on the expression of immunity-related genes and the number of hemocytes in uninfected and Escherichia coli -infected workers of the stingless bee Melipona quadrifasciata . Expression of the immune-related gene vitellogenin decreased and the number of circulating hemocytes increased in bioinsecticide-exposed uninfected bees and in unexposed E. coli -infected bees as compared with unexposed uninfected bees. Our results show that though bioinsecticides are considered safe, contamination by sublethal doses of azadirachtin and spinosad can interfere with the immune system of bees.bioinsecticides / hemocytes / spinosad / stingless bees / vitellogenin

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Section: Total Hemocyte Countmentioning

confidence: 99%

Changes in innate immune response and detoxification in Melipona quadrifasciata (Apinae: Meliponini) on oral exposure to azadirachtin and spinosad

et al. 2020

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“…(2013). Since then infections by the DWV-A in colonies of the South-eastern region of Buenos Aires Province have been detected from our research group (Brasesco et al ., 2013; Negri et al ., 2015; Quintana et al ., 2019). Even so, the information is still scarce and fragmented, and the presence of DWV-B in colonies of bees of Argentina has not been reported so far.…”

Section: Discussionmentioning

confidence: 99%

“…(2008), for the amplification of the conserved region of the RNA-dependent RNA polymerase (RdRp) gene, were used. This primer pair has been used in previous studies with good results (Negri et al ., 2015; Quintana et al ., 2019). For the amplifications of fragments of the DWV-B, we used two primer pairs designed by Zioni et al .…”

Section: Methodsmentioning

confidence: 99%

“…High loads of DWV in bees with deformed wings and lower viral loads in bees with normal wing appearance were observed, as previously reported (Yang and Cox-Foster, 2005; Martin et al ., 2012). This was also corroborated later on by our research group in a recently published article; differences in DWV loads between deformed wings bees and normal bees were analyzed in relation with changes in the expression levels of immune genes of A. mellifera (Quintana et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deformed wing virus type a and b in managed honeybee colonies of Argentina

Brasesco

Quintana

Gerónimo³

et al. 2020

Bull. Entomol. Res.

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Apis mellifera is infected by more than 24 virus species worldwide, mainly positive-sense, single-stranded RNA viruses of the Dicistroviridae and Iflaviridae families. Among the viruses that infect honey bees, Deformed wing virus is the most prevalent and is present as three master variants DWV-A, B, and C. Given that the ectoparasitic mite Varroa destructor vectors these virus variants, recombination events between them are expected, and variants and their recombinants can co-exist in mites and honeybees at the same time. In this study, we detect, through RT-qPCR, the presence of DWV-A and B in the same samples of adult bees from colonies of Argentina. Total RNA was extracted from pools of ten adult bees from 45 apiaries distributed across the main beekeeping Provinces of Argentina (Buenos Aires, Santa Fe, Córdoba, Santiago del Estero, Río Negro, and Mendoza); then RT-qPCR reactions were performed to detect DWV-A and B, with specific primer pairs. After the amplifications, PCR products (204 and 660 bp amplicons for DWV-B, and ~250 bp for DWV-A) were purified and sequenced to verify that they corresponded to reported sequences, analyzing them using the Blast software. Of the 45 samples analyzed by RT-qPCR, over 90% were infected with DWV-A and 47% were also positive for DWV-B, where it was found in high prevalence specifically in colonies of A. mellifera of the Buenos Aires Province. Future studies will determine the impact of this type of the virus and its ability to recombine with the other DWV types in the apiaries of our country.

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“…These results provide additional evidence for the active immune suppression by the DWV associated with the Toll pathway. Recently, Quintana et al [156] found that bees with deformed wings and increased levels of DWV have upregulated expression of the genes Domeless and TEPa (JAK-STAT pathway), and also NOS . Interestingly, in the same study, no differences in Toll Wheeler (Toll18W) mRNA levels are found, but, an up-regulation of the NF-κB inhibitor (IκB) gene Cactus is detected in bees with deformed wings and increased levels of DWV.…”

Section: Varroa-dwv Within the Context Of Innate Immune Responsesmentioning

confidence: 99%

Towards Precision Nutrition: A Novel Concept Linking Phytochemicals, Immune Response and Honey Bee Health

Negri

Villalobos

Szawarski

et al. 2019

Insects

Self Cite

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The high annual losses of managed honey bees (Apis mellifera) has attracted intensive attention, and scientists have dedicated much effort trying to identify the stresses affecting bees. There are, however, no simple answers; rather, research suggests multifactorial effects. Several works have been reported highlighting the relationship between bees’ immunosuppression and the effects of malnutrition, parasites, pathogens, agrochemical and beekeeping pesticides exposure, forage dearth and cold stress. Here we analyze a possible connection between immunity-related signaling pathways that could be involved in the response to the stress resulted from Varroa-virus association and cold stress during winter. The analysis was made understanding the honey bee as a superorganism, where individuals are integrated and interacting within the colony, going from social to individual immune responses. We propose the term “Precision Nutrition” as a way to think and study bees’ nutrition in the search for key molecules which would be able to strengthen colonies’ responses to any or all of those stresses combined.

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Up-regulated pathways in response to Deformed Wing Virus infection in Apis mellifera (Hymenoptera: Apidae)

Cited by 20 publications

References 56 publications

Changes in innate immune response and detoxification in Melipona quadrifasciata (Apinae: Meliponini) on oral exposure to azadirachtin and spinosad

Changes in innate immune response and detoxification in Melipona quadrifasciata (Apinae: Meliponini) on oral exposure to azadirachtin and spinosad

Deformed wing virus type a and b in managed honeybee colonies of Argentina

Towards Precision Nutrition: A Novel Concept Linking Phytochemicals, Immune Response and Honey Bee Health

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