Update of reference values for IgG antibodies against typical antigens of hypersensitivity pneumonitis

Raulf, Monika; Joest, M.; Sander, I.; Hoffmeyer, Frank; Nowak, Dennis; Ochmann, Uta; Preisser, Alexandra M.; Schreiber, Jens; Sennekamp, J.; Koschel, Dirk

doi:10.1007/s40629-019-0099-x

Cited by 29 publications

(14 citation statements)

References 16 publications

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“…The clinically relevant concentration for detecting antibodies varies depending on the application. For the detection of antibodies used for the diagnosis of infectious diseases (Fiorentini et al, 2008;Rostenberg and Peñ aloza, 1978;Barletta et al, 2004;Raulf et al, 2019), the concentration level expected to have in clinical samples (i.e. blood, serum) is -depending on the stage of infection -in the pM to low nM range.…”

Section: Discussionmentioning

confidence: 99%

Single antibody detection in a DNA origami nanoantenna

et al. 2021

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DNA nanotechnology offers new biosensing approaches by templating different sensor and transducer components. Here, we combine DNA origami nanoantennas with label-free antibody detection by incorporating a nanoswitch in the plasmonic hotspot of the nanoantenna. The nanoswitch contains two antigens that are displaced by antibody binding, thereby eliciting a fluorescent signal. Singleantibody detection is demonstrated with a DNA origami integrated anti-digoxigenin antibody nanoswitch. In combination with the nanoantenna, the signal generated by the antibody is additionally amplified. This allows the detection of single antibodies on a portable smartphone microscope. Overall, fluorescence-enhanced antibody detection in DNA origami nanoantennas shows that fluorescence-enhanced biosensing can be expanded beyond the scope of the nucleic acids realm.

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Section: Discussionmentioning

confidence: 99%

Single antibody detection in a DNA origami nanoantenna

et al. 2021

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“…For simple aspergilloma in pulmonary tuberculosis patients, the same working group found a sensitivity of 63.5% and a specificity of 98.3% using a cut-off of 27.3 mg/L. However, the Gm3 cut-off-level is still under discussion, as a recent multicentric study found a median Gm3 of 21.3 mg/L and a 90% quantile for Gm3 of 78 mg/L in a population of 121 healthy donors (Raulf et al, 2019). The working group suggested that IgG values higher than at least 90% of the healthy donor group indicate elevated levels, which is more consistent with our findings of a cut-off-level of 78.05 mg/L.…”

Section: Discussionmentioning

confidence: 99%

Prospective Evaluation of Aspergillus fumigatus-Specific IgG in Patients With Cystic Fibrosis

Eschenhagen

Grehn

Schwarz

2021

Front. Cell. Infect. Microbiol.

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BackgroundIn Cystic Fibrosis (CF), the airways are often colonized by opportunistic fungi. The most frequently detected mold is Aspergillus fumigatus (Af). Af diseases are associated with significant morbidity and mortality. The most common clinical picture caused by Af is allergic bronchopulmonary aspergillosis (ABPA), triggered by an immunological reaction against Af. Af bronchitis and invasive aspergillosis rarely occur in CF as a result of spore colonization and germination. Since pulmonary mycoses and exacerbations by other pathogens overlap in clinical, radiological, and immunological characteristics, diagnosis still remains a challenge. The search for reliable, widely available biomarkers for Af diseases is therefore still an important task today.ObjectivesAf-specific IgG m3 is broadly available. Sensitivity and specificity data are contradictory and differ depending on the study population. In our prospective study on pulmonary Af diseases in CF, we determined specific IgG m3 in order to test its suitability as a biomarker for acute Af diseases and as a follow-up parameter.MethodsIn this prospective single center study, 109 patients with CF were screened from 2016 to 2019 for Af-associated diseases. According to diagnostic criteria, they were divided into four groups (control, bronchitis, ABPA, pneumonia). The groups were compared with respect to the level of Af-specific IgG (ImmunoCAP Gm3). We performed a receiver operating characteristic (ROC) curve analysis to determine cut-off, sensitivity and specificity. Twenty-one patients could be enrolled for a follow-up examination.ResultsOf the 109 patients, 36 were classified as acute Af-disease (Af bronchitis, ABPA, Af pneumonia). Of these, 21 patients completed follow up-screening. The median Af-specific Gm3 was higher in the acute Af-disease groups. There was a significant difference in Af-specific IgG m3 compared to the control group without acute Af-disease. Overall, there was a large interindividual distribution of Gm3. A cut-off value of 78.05 mg/L for Gm3 was calculated to discriminate controls and patients with ABPA/pneumonia with a specificity of 75% and a sensitivity of 74.6%. The follow up examination of 21 patients showed a decrease of Gm3 in most patients without statistical significance due to the small number of follow up patients.ConclusionAf specific IgG may be a useful biomarker for acute ABPA and Af pneumonia, but not for Af bronchitis in CF. However, due to the large interindividual variability of Gm3, it should only be interpreted alongside other biomarkers. Therefore, due to its broad availability, it could be suitable as a biomarker for ABPA and Af pneumonia in CF, if the results can be supported by a larger multicenter cohort.

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“…Several studies have suggested the presence of cross-reactive antigenic reactions between different bird species or different avian matrices, especially for pigeons 14,[27][28][29] . Recently, significant correlations have been found in serological analyses between pigeon, duck, and goose antigens 11 . Based on the BLASTp alignment, our results showed that the amino-acid sequence of duck IGLL has higher identity with goose IGLL (ID: 81%) than pigeon IGLL1 (ID: 65%) (Supplemental data).…”

Section: Discussionmentioning

confidence: 99%

“…The IGLL1 protein has been identified in droppings, bloom, and pigeon serum and PROE protein in droppings and bloom. A strong correlation has been shown between the IgG antibody response of patients sensitized to pigeon, duck and goose antigens 11 . Therefore, we hypothesize that amino-acid sequences close to those found in IGLL1 and PROE are part of proteins from the feathers (goose, duck) used for the manufacture of pillows and duvets.…”

Section: Introductionmentioning

confidence: 96%

“…A recent modified Delphi survey on chronic HP shows that exposure to a causative antigen is the most important clinical variable supporting a confident diagnosis 3 . Although the use of serological tests is not universally accepted, they can be used to demonstrate antigenic exposure by looking for circulating precipitins or IgG antibodies 3,11,12 .…”

Section: Introductionmentioning

confidence: 99%

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Recombinant antigens r-PROE and r-IGLL1 for serodiagnosis of feather duvet lung

Rouzet¹,

Morell²,

Reboux³

et al. 2020

Preprint

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Background: Feather duvet lung (FDL) is an underestimated form of acute and chronic hypersensitivity pneumonitis. Serological tests for FDL need to be validated. We investigated the ability of recombinant pigeon Proproteinase E (r-PROE) and Immunoglobulin-lambda-like-polypeptide-1 (r-IGLL1) proteins to support the serological diagnosis of FDL, and propose them as a serological tool for clinicians to differentiate cases from FDL and Bird fancier's lung (BFL). Methods: Specific IgG antibodies against r-PROE and r-IGLL1, analyzed with ELISA, were measured in patients diagnosed with FDL (n=31), BFL (n=15) controls exposed (n=15) and unexposed to feathers (n=15). Results: The sensitivity and specificity of the r-PROE ELISA for the serological diagnosis of FDL cases versus exposed and unexposed controls were 74.2% and 86.7% respectively, with an index threshold of 0.5. (AUC: 0.9). In addition, this serological test was effective to support the serological diagnosis of FDL and BFL cases with significantly different thresholds. The r-IGLL1 ELISA was only effective for the serological diagnosis of BFL. Also, these two serological tests were useful for the diagnosis of both chronic and acute forms. Conclusions: The new diagnostic test for FDL using r-PROE protein should help to detect overt and hidden cases of FDL. The combination of both test will help the clinician in distinguish between the etiology of birds or feathers duvet.

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Update of reference values for IgG antibodies against typical antigens of hypersensitivity pneumonitis

Cited by 29 publications

References 16 publications

Single antibody detection in a DNA origami nanoantenna

Single antibody detection in a DNA origami nanoantenna

Prospective Evaluation of Aspergillus fumigatus-Specific IgG in Patients With Cystic Fibrosis

Recombinant antigens r-PROE and r-IGLL1 for serodiagnosis of feather duvet lung

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