Update on Immunohistochemical Methods Relevant to Dermatopathology

Wasserman, Justin; Maddox, Jessica; Racz, Mark; Petronic‐Rosic, Vesna

doi:10.5858/133.7.1053

Cited by 25 publications

(9 citation statements)

References 61 publications

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“…For example, genomic analysis of cutaneous melanoma has shown unique patterns of mutation linked to various subtypes and stages of the illness. Not only has the discovery of mutations in genes like BRAF, NRAS, and KIT facilitated accurate subtyping, but it has also spurred the creation of targeted medicines that specifically target these alterations [3]. Similar to this, molecular profiling has revealed mutations in genes producing structural proteins essential for skin integrity in illnesses such as epidermolysis bullosa (EB), providing insight on the underlying pathophysiology and opening the door for future gene-based therapeutics [4].…”

Section: Section 1: Dermatopathology Molecular Profilingmentioning

confidence: 99%

“…These kinds of partnerships encourage a multidisciplinary strategy by combining knowledge from many domains to address the complexity of skin disorders. This collaboration fosters innovation and guarantees a patient-centric approach in dermatopathology by expediting the translation of research results into clinical practice [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…The understanding of different skin disorders has been revolutionised by advances in molecular profiling techniques. Extensive genetic fingerprints underlying dermatological disorders have been discovered by studies employing next-generation sequencing (NGS) and other high-throughput technologies, making it possible to precisely classify and delineate disease subtypes [3]. The development of targeted medicines and personalised treatment methods has been accelerated by the identification of certain genetic abnormalities, such as mutations in genes encoding critical signalling pathways or variations in regulatory elements [4].…”

Section: Introductionmentioning

confidence: 99%

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Updates in Dermatopathology: Emerging Trends in Diagnosis and Classification

Asma Hussain

2023

IJRITCC

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Developments in dermatopathology are transforming clinical practice profoundly by constantly changing disease categorization and diagnostic methods. This review highlights key developments that have shaped modern dermatopathology, including the use of digital pathology, artificial intelligence (AI) applications, changing categorization schemes, and molecular profiling. By using methods such as next-generation sequencing, molecular profiling clarifies complex genetic changes that underlie skin conditions, enabling accurate diagnosis and focused treatment plans. Digital pathology systems are transforming the field of diagnostics through the provision of remote consultations, cooperation, and improved diagnosis accuracy. Artificial intelligence (AI) applications show promise in automating picture processing, improving diagnosis accuracy, and optimising workflow. Molecular, histological, and clinical data are integrated via evolving classification systems, which improve disease categorization and prognostication. The synergy of knowledge created by interdisciplinary interactions between dermatologists, pathologists, technologists, and molecular biologists promotes holistic approaches to patient treatment and disease understanding. When taken as a whole, these discoveries represent a paradigm change in dermatopathology, opening the door to applications in precision medicine and individualised patient care.

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Section: Section 1: Dermatopathology Molecular Profilingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Updates in Dermatopathology: Emerging Trends in Diagnosis and Classification

Asma Hussain

2023

IJRITCC

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“…Finally, the final diagnosis should take into consideration the clinical history and histological diagnosis as well as IH panel (Wassermann et al 2009) (Figs. 18 and 19).…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

“…IH markers used in dermatopathology. (Note: no marker is completely specific for a tumor(Edward and Yung 2012;Wassermann et al 2009;Kempf et al 2008;Logan 2007)) …”

mentioning

confidence: 99%

Histopathology of the Skin: General Principles

Ladoyanni

2021

Atlas of Dermatology, Dermatopathology and Venereology

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Dermatopathology deals with the microscopic study of skin, adjacent mucosa, and cutaneous adnexal structures. Dermatology and dermatopathology put much emphasis on clinical and pathological features and rely heavily upon the powers of observation and correct classification in order to obtain the correct diagnosis. Both specialties are in fact complementary and closely related to each other. A fundamental understanding of dermatopathological changes is necessary if one wishes to become an effective dermatologist. In this chapter, the histopathological basic principles of the skin are discussed.

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A cellular model reflecting the phenotypic heterogeneity of mutant HRAS driven squamous cell carcinoma

Cantariño

Fernández‐Figueras

Valero

et al. 2016

Intl Journal of Cancer

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Squamous cell carcinomas have a range of histopathological manifestations. The parameters that determine this clinically observed heterogeneity are not fully understood. Here, we report the generation of a cell culture model that reflects part of this heterogeneity. We have used the catalytic subunit of human telomerase hTERT and large T to immortalize primary UVunexposed keratinocytes. Then, mutant HRAS G12V has been introduced to transform these immortal keratinocytes. When injected into immunosuppressed mice, transformed cells grew as xenografts with distinct histopathological characteristics. We observed three major tissue architectures: solid, sarcomatoid and cystic growth types, which were primarily composed of pleomorphic and basaloid cells but in some cases displayed focal apocrine differentiation. We demonstrate that the cells generated represent different stages of skin cancerogenesis and as such can be used to identify novel tumor-promoting alterations such as the overexpression of the PADI2 oncogene in solid-type SCC. Importantly, the cultured cells maintain the characteristics from the xenograft they were derived from while being amenable to manipulation and analysis. The availability of cell lines representing different clinical manifestations opens a new tool to study the stochastic and deterministic factors that cause case-to-case heterogeneity despite departing from the same set of oncogenes and the same genetic background.Keratinocyte carcinomas are by far the most common malignancies worldwide and their incidence and morbidity continuously increase, specially in light-skinned populations.1,2 The two main subtypes are basal cell carcinomas and squamous cell carcinomas (SCC). Basal cell carcinomas are the most frequent among all cancers. They are slow-growing, largely noninvading tumours that are caused by an aberrant sonic hedgehog pathway. 2 SCC are tumours with interfollicular epidermal differentiation which are more prone to spread and can be originated by a wide range of genetic mechanisms. 3 Solar UV radiation is the main risk factor for SCC. 4 Additional risk factors include infection with human papillomavirus, iatrogenic immunosuppression after solid-organ transplantation and several different chemicals such as the nitrosamines found in tobacco smoke. 2 SCCs evolve particularly fast as a consequence of targeted melanoma therapy. 5 Almost half of patients with metastatic melanoma patients carry the BRAFV600E mutation and are candidates for treatment with the BRAF inhibitors vemurafenib and dabrafenib. 5-7 SCC and related keratoacanthoma appear in up to 26% of treated melanoma patients as a sudden and adverse secondary effect. 5,7 It is thought that these tumours arise through the fast clonal expansion of premalignant keratinocytes carrying mutant

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Update on Immunohistochemical Methods Relevant to Dermatopathology

Cited by 25 publications

References 61 publications

Updates in Dermatopathology: Emerging Trends in Diagnosis and Classification

Updates in Dermatopathology: Emerging Trends in Diagnosis and Classification

Histopathology of the Skin: General Principles

A cellular model reflecting the phenotypic heterogeneity of mutant HRAS driven squamous cell carcinoma

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