Update on the Major Clonal Types of Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            in the Czech Republic

Melter, Oto; Aires-de-Sousa, Marta; Urbášková, P; Jakubů, Vladislav; Žemličková, Helena; Lencastre, Hermı́nia de

doi:10.1128/jcm.41.11.4998-5005.2003

Cited by 70 publications

(84 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In England, the percentage increased from 1.5 to 13.2% between 1989 and 1995 (8)(9)(10)(11)(12)(13). In Brazil, the prevalence of MRSA is high, especially in large and teaching hospitals, ranging from 26.6 to 70%, and MRSA is considered to be the main pathogen causing hospital outbreaks (14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

Beretta

Trabasso

Stucchi

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related profiles were also identified. During period II, 80 (68.8%) of 117 strains showed the same endemic profile A identified during period I, 18 (13.7%) closely related profiles and 18 (13.7%) possibly related profiles and, only one strain presented an unrelated profile. Cluster analysis showed a 96% coefficient of similarity between profile A from period I and profile A from period II, which were considered to be from the same clone. The molecular monitoring of MRSA strains permitted the determination of the clonal dissemination and the maintenance of a dominant endemic strain during a 10-year period and the presence of closely and possibly related patterns for endemic profile A. However, further studies are necessary to improve the understanding of the dissemination of the endemic profile in this hospital.

show abstract

Section: Introductionmentioning

confidence: 99%

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

Beretta

Trabasso

Stucchi

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…A second multiresistant clone (Brazilian Epidemic clone -BEC) was shown to be widely spread in Brazilian hospitals several thousands of kilometers apart [11] and later it was also associated with infections in Portugal, Argentina, Uruguay, Czech Republic, and Canada [12][13][14][15]. Several other studies [16][17][18] in Brazil, using pulsed-field gel electrophoresis (PFGE) technique, to analyze Brazilian MRSA strains, also indicated a low variability between strains.…”

mentioning

confidence: 99%

Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil

Cury¹,

Mobilon²,

Stehling³

et al. 2009

Braz J Infect Dis

View full text Add to dashboard Cite

One hundred and fifty-one methicillin-resistant Staphylococcus aureus (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in Campinas were grouped in clusters A and B, while most of the strains isolated in Ribeirão Preto were grouped in cluster C. This distribution denotes the existence of different founder strains that undergo independent genetic variability. The strains considered representative of the Brazilian Epidemic Clone (BEC) were categorized as cluster A. These results indicate a possible higher variability among Brazilian MRSA strains than currently described and indicate that the techniques herein used can be used as an alternative to Pulsed Field Gel Electrophoresis (PFGE).

show abstract

“…However, differences between the PFGE protocol used by Sader et al and the other authors mentioned above makes the comparison of their results impossible. Later on, the BC was reported in Portugal , Argentina, Uruguay, Chile , and the Check Republic (Melter et al 2003).…”

Section: Evolution Of Typing Techniques and The Characterization Of Mmentioning

confidence: 99%

“…This MRSA clone was first described in Spain, 1989(Dominguez et al 1994) and later on, in Portugal (Ferrari et al 2002), Italy and United Kingdom (Mato et al 1998), Germany (Witte et al 1994), Belgium, Switzerland, France (Deplano et al 2000), Poland (Leski et al 1998), Check Republic (Melter et al 2003), and United States (Roberts et al 1998a).…”

Section: Evolution Of Typing Techniques and The Characterization Of Mmentioning

confidence: 99%

Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

Vivoni

Moreira

2005

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Update on the Major Clonal Types of Methicillin-Resistant Staphylococcus aureus in the Czech Republic

Abstract: The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus

Cited by 70 publications

References 34 publications

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in two metropolitan areas of São Paulo State, southeast Brazil

Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

Contact Info

Product

Resources

About