Update on White Lupin Cluster Root Acclimation to Phosphorus Deficiency Update on Lupin Cluster Roots

Cheng, Lingyun; Bucciarelli, Bruna; Shen, Jianbo; Allan, Deborah L.; Vance, Carroll P.

doi:10.1104/pp.111.175174

Cited by 73 publications

(42 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…translocated from source leaves is part of a systemic signalling system that leads to Pi deficiency-induced alterations in root morphology and metabolism in white lupin and other plant species, including arabidopsis (Liu et al, 2005;Hammond and White, 2008;Cheng et al, 2011Cheng et al, , 2014Wang et al, 2014). Here, we have established a novel mechanistic link between reversible in vivo PEPC phosphorylation and the concentrations of sucrose and its signalling molecule Tre6P (Yadav et al, 2014), while correlating this interesting phenomenon with the diurnal control of organic anion exudation and Pi uptake by CRs of ÀPi white lupin plants (Watt and Evans, 1999;Hocking and Jeffery, 2004;Liu et al, 2005;Cheng et al, 2014).…”

Section: Discussionmentioning

confidence: 90%

“…The upregulation of PEPC during Pi starvation has also been linked to the synthesis and exudation of large quantities of organic acid anions (e.g. malate, citrate) by roots of various ÀPi plants (Neumann et al, 2000;Vance et al, 2003;Shane and Lambers, 2005;Plaxton and Tran, 2011;Cheng et al, 2011;Lambers et al, 2011). This greatly increases root Pi acquisition by solubilizing otherwise inaccessible sources of mineralized soil Pi, thus increasing soluble Pi concentrations by up to 1000-fold (Vance et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Transcriptional expression of various Pi-responsive genes in white lupin CRs (including that encoding the dominant PEPC isozyme LaPEPC3 (PEPC isozyme-3 from L. albus)) also increased in the light and decreased in the dark, suggesting that light-driven changes in the supply of photosynthate (sucrose) from the leaves to the CRs regulated expression of these genes in the latter (Liu et al, 2005;Cheng et al, 2011Cheng et al, , 2014. The data support the view that shoot carbohydrate supply plays a crucial role in orchestrating root metabolic responses to Pi limitation, including white lupin CRs (Liu et al, 2005;Hammond and White, 2008;Cheng et al, 2011Cheng et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Despite numerous physiological, biochemical and transcriptomic studies of white lupin Pi-starvation responses Vance et al, 2003;Liu et al, 2005;Cheng et al, 2011Cheng et al, , 2014, there is little information about regulatory PEPC phosphorylation in this species. PEPC was suggested to be activated by phosphorylation in CRs of ÀPi white lupin since the partially purified enzyme from this tissue displayed decreased sensitivity to malate inhibition following its incubation with ATP (Uhde- .…”

Section: Introductionmentioning

confidence: 99%

“…This adaptive strategy is especially prevalent in highly specialized cluster (proteoid) roots (CRs), which mediate efficient Pi acquisition by certain plant species acclimating to soluble Pi-impoverished soils (Keerthisinghe et al, 1998;Neumann et al, 2000;Watt and Evans, 1999;Shane and Lambers, 2005;Cheng et al, 2011;Lambers et al, 2011). Of the various plants that form CRs, white lupin (Lupinus albus) stands out as an important agricultural crop, and is the species that has been most intensively investigated with respect to Pi nutrition (Cheng et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Shane

Feil

Lunn

et al. 2016

Ann Bot

View full text Add to dashboard Cite

Background and Aims Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated enzyme that controls carbohydrate partitioning to organic acid anions (malate, citrate) excreted in copious amounts by cluster roots of inorganic phosphate (Pi)-deprived white lupin plants. Excreted malate and citrate solubilize otherwise inaccessible sources of mineralized soil Pi for plant uptake. The aim of this study was to test the hypotheses that (1) PEPC is post-translationally activated by reversible phosphorylation in cluster roots of illuminated white lupin plants, and (2) light-dependent phosphorylation of cluster root PEPC is associated with elevated intracellular levels of sucrose and its signalling metabolite, trehalose-6-phosphate.Methods White lupin plants were cultivated hydroponically at low Pi levels ( 1 mM) and subjected to various light/ dark pretreatments. Cluster root PEPC activity and in vivo phosphorylation status were analysed to assess the enzyme's diurnal, post-translational control in response to light and dark. Levels of various metabolites, including sucrose and trehalose-6-phosphate, were also quantified in cluster root extracts using enzymatic and spectrometric methods.Key Results During the daytime the cluster root PEPC was activated by phosphorylation at its conserved Nterminal seryl residue. Darkness triggered a progressive reduction in PEPC phosphorylation to undetectable levels, and this was correlated with 75-80 % decreases in concentrations of sucrose and trehalose-6-phosphate.Conclusions Reversible, light-dependent regulatory PEPC phosphorylation occurs in cluster roots of Pi-deprived white lupin plants. This likely facilitates the well-documented light-and sucrose-dependent exudation of Pi-solubilizing organic acid anions by the cluster roots. PEPC's in vivo phosphorylation status appears to be modulated by sucrose translocated from CO 2 -fixing leaves into the non-photosynthetic cluster roots.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Shane

Feil

Lunn

et al. 2016

Ann Bot

View full text Add to dashboard Cite

show abstract

Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review

Vranová

Rejšek

Skene

et al. 2013

Z. Pflanzenernähr. Bodenk.

180

111

View full text Add to dashboard Cite

The aim of this work is to review the current knowledge on the effects of plant metabolism (C3, C4, and CAM) on root exudation and on the methods of exudate collection as well as the use of such exudates for analyses, testing of microbial response, degradation of pollutants, enzymatic activities, and occurrence of allelochemicals. We examine the advantages and disadvantages of each method as related to the downstream use of the exudates. The use of continuous percolation of solid cultivation medium with adjustment of nutrient‐solution strength appears to be a promising methodology for the determination of root exudation rates and qualitative composition of exuded compounds. The method mimics rhizosphere conditions, minimizing the artificial accumulation of compounds, alteration of plasma‐membrane permeability, ATPase activity, and the impacts of inhibitors or stimulators of root enzymes. Of particular significance is the fact that the adjustment of strength of nutrient solution and percolation enables universal and also long‐term use of the method, allowing high exudation yield by minimizing influx and maximizing efflux rates of exuded compounds at high nutrient‐solution strength. Furthermore, it facilitates assessment of the effect on soil microbial populations and their ability to degrade pollutants. Enzymatic activities can be assessed when a low strength of nutrient solution is used, with percolation of the exudates directly into tested soils. Composition of root exudates, regulation of root enzymes, and plant response to nutrient deficiency can be assessed by measuring net efflux or influx rates. The impact of heavy metals and other type of mechanical, chemical, and biological stresses differs according to the type of plant metabolism. This has significant consequences on transformations in plant communities, both structurally and functionally, and impacts upon crop nutrition, with respect to global climate change, and the use of plants for phytoremediation purposes. Understanding the effects of different types of plant metabolism on root exudation with respect to genetic regulation of synthetic pathways through root enzymes and transport systems presents an important direction for future research.

show abstract

Genomic resources for lupins are coming of age

et al. 2021

View full text Add to dashboard Cite

Lupins are underutilised pulse crops subject to increasing interest for human consumption of the high‐protein grain. They are also valued as a source of animal nutrition and make an excellent break crop in agricultural production systems. Like other orphan legumes, the genomic revolution has made it cost‐effective to also apply modern genetic and genomic approaches in lupins. These have predominantly been conducted in the two major domesticated lupin species, namely, narrow‐leafed lupin (NLL; Lupinus angustifolius) and white lupin (Lupinus albus), with transcriptome studies also emerging in other domesticated and undomesticated species. This review provides an overview of the current lupin genomic resources developed including two reference genomes for NLL and white lupin, several transcriptome resources and the development of pan‐genomes for NLL and white lupin, and describes how these offer great potential to increase grain yield and quality for these recently domesticated pulse crops. Furthermore, we highlight the importance of lupins to further our understanding of many aspects of fundamental legume biology. Combined, this will aid breeders and growers to improve lupin crops to help meet the increasing demand for plant protein in more sustainable cropping systems.

show abstract

Update on White Lupin Cluster Root Acclimation to Phosphorus Deficiency Update on Lupin Cluster Roots

Cited by 73 publications

References 96 publications

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review

Genomic resources for lupins are coming of age

Contact Info

Product

Resources

About