Updated Functional Classification of β-Lactamases

Bush, Karen; Jacoby, George A.

doi:10.1128/aac.01009-09

Cited by 2,028 publications

(1,777 citation statements)

References 73 publications

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“…3 β-lactamases including broad-and extended-spectrum β-lactamases (ESBLs) have been reported predominantly in Enterobacteriaceae, especially Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli). [4][5][6] Most extended-spectrum β-lactamases fall into three genotypes: TEM, SHV and CTX-M 7 with hundreds of variants of these gene families having emerged and disseminated widely requiring concerted efforts to effectively control. 6,8 An example is the 4130 allelic variants of CTX-M (Lahey Clinic website: http://www.lahey.org/Studies/other.asp#table1).…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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Resistance to β-lactam antibiotics through β-lactamase production by Enterobacteriaceae continues to burden the health-care sector worldwide. Traditional methods for detection of β-lactamases are time-consuming and labor-intensive and newer methods with varying capabilities continue to be developed. The objective of this study was to develop a multiplex PCR (M-PCR) system for the detection of bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes and to apply it in clinical Klebsiella pneumoniae and Escherichia coli strains. To do this, we used group-specific PCR primers in singleplex reactions followed by optimization into multiplex reactions. Specificity and sensitivity of the M-PCR were then evaluated using 58 reference strains before its application to detect bla group genes in 203 clinical Enterobacteriaceae strains. PCR amplicons were sequenced to determine the β-lactamase subtypes. The M-PCR system exhibited 100% specificity and sensitivity. In all, 83.7% of K. pneumoniae and 89.8% of E. coli clinical strains harbored bla group genes with 46.9%, 40.1%, 15.0%, 21.1% and 6.1% of K. pneumoniae having bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively, whereas 12.2%, 77.6%, 22.4%, 36.7% and 8.2% of E. coli had bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively. Bla SHV-1 , bla SHV-11 , bla SHV-27 , bla SHV-33 , bla SHV-144 , bla TEM-1 , bla TEM-135 , bla OXA-1 , bla CTX-M-3 , bla CTX-M-9 , bla CTX-M-14 , bla CTX-M-15 , bla CTX-M-27 , bla CTX-M-55 , bla CTX-M-65 and bla CTX-M-104 were detected. In conclusion, the M-PCR system was efficient and versatile with an advantage of simultaneously detecting all the targeted bla group genes. Hence, it is a potential candidate for developing M-PCR kits for the screening of these genes for clinical or epidemiological purposes.

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Section: Introductionmentioning

confidence: 99%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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“…Durante la última década, se ha reportado a nivel mundial un aumento sostenido en el porcentaje de bacterias Gram negativas que son resistentes a todas las familias de antibióticos y especialmente a los carbapenem (1,4,5). Para que una bacteria Gram negativa exprese un fenotipo resistente a los carbapenem, requiere de la combinación de, al menos, dos mecanismos de resistencia, siendo la más frecuente la producción de enzimas y el cierre de porinas, lo cual, además, le confiere a la bacteria resistencia a otros betalactámicos (6)(7)(8).…”

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“…Las enzimas más relevantes en la resistencia a los carbapenem son las betalactamasas de tipo AmpC y las carbapenemasas (7,9,10), entre las que se encuentran las KPC, enzimas principalmente codificadas por el gen bla KPC , el cual generalmente se localiza en plásmidos. La localización en dicho elemento genético móvil le confiere a las bacterias portadoras la capacidad de compartir esta información genética con otras especies y familias de bacterias (11), facilitando su diseminación 'clonal' (12) y geográfica (13).…”

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Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

et al. 2013

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34(Supl.1):81-90 Prevalencia de bacterias portadoras de blaKPC en Colombia Contribución de los autores:Robinson Pacheco: realización del estudio, recolección de la información clínica y epidemiológica, realización de los análisis estadísticos y redacción del manuscrito. Lyda Osorio: participación en el diseño del estudio, supervisión de la recolección de información y del análisis estadístico. Adriana M. Correa: coordinación de las actividades de laboratorio, desarrollo de la técnica molecular de PFGE. María Virginia Villegas: participación en el diseño y coordinación general del estudio. Todos los autores participaron en la revisión y corrección del manuscrito. ARTÍCULO ORIGINAL Biomédica 2014;34(Supl.

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“…This enzyme will be more devastating if its gene is incorporated into an integron capable of encoding resistance to all the antibiotics commonly used in therapeutics. [8][9][10][11] For practical purposes, a bacterial strain is said to be multidrug-resistant if it is resistant to at least three classes of potentially active antibiotics. It is said to be extensively drug-resistant if it is resistant to all but one or two antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Fosfomycin and Its Application in the Treatment of Multidrug-Resistant Enterobacteriaceae Infections

2011

Clinical Medicine Reviews in Therapeutics

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Abstract:The use of fosfomycin has been limited in therapeutics in recent years. Because it has shown good antibacterial activity in vitro and clinical efficacy in some domains, it has been proposed as an alternative to current antimicrobial agents, which are subject to increasing resistance. This paper reviews the main properties of fosfomycin and the latest publications concerning multidrugresistant Enterobacteriaceae infections. In uncomplicated urinary tract infections, a single oral dose was found to be safe and effective. In complicated urinary tract infections, the same results were observed with several doses. In both cases, by using fosfomycin to treat infections, the use of carbapenems could be reduced, leading to lower costs and better microbial ecology. In severe infections, combinations with intravenous fosfomycin need to be explored further because its future activity may depend on choosing a good partner drug. Because of the fast evolution of microbial resistance, more studies are urgently needed.

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Updated Functional Classification of β-Lactamases

Cited by 2,028 publications

References 73 publications

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

Fosfomycin and Its Application in the Treatment of Multidrug-Resistant Enterobacteriaceae Infections

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