UPLC-PDAD Analysis for Simultaneous Determination of Ten Synthetic Preservatives in Foodstuff

Li, Xiuqin; Ji, Chao; Yong, Wei; Ling, Yun; Yang, Mei; Chu, Xiaogang

doi:10.1365/s10337-008-0645-z

Cited by 13 publications

(7 citation statements)

References 21 publications

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“…To prevent Salmonella contamination and growth, synthetic preservatives, such as ethylparaben, methylparaben, benzoice acid, sorbic acid, and propanoic acid, are added to foodstuffs, including meat products [ 38 ]. Although synthetic preservatives are effective and economical, many have reported harmful effects [ 39 ], and thus, there is a considerable demand for a safe and economical natural preservative.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Lee

Yun

Goo

2020

Insects

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The expressions of antimicrobial peptides (AMPs) in the larvae of the black soldier fly, Hermetia illucens, were significantly increased by pathogen or stimulant induced innate immunity activation. We immunized H. illucens fifth instar larvae with five different Lactobacillus species, that is, Lactobacillus acidophilus, L. brevis, L. casei, L. fermentum, or L. delbrueckii, to induce the mass production of AMPs and selected optimal immune inducers. Antimicrobial activities in hemolymph and H. illucens larvae (HIL) extract were evaluated against three salmonella species (Salmonella pullorum, Salmonella typhimurium, and Salmonella enteritidis). Highest antimicrobial activity was shown by the hemolymph of HIL immunized by L. casei and its activity was closely linked with the inductions of cecropin 1 (HiCec1) and defensin 1 (HiDef1) gene expressions. Furthermore, antimicrobial activity in hemolymph was stable to heat and pH and the growth of three Salmonella species were dramatically suppressed by HIL hemolymph and extract after immunization with L. casei. The minimal inhibitory concentration (MICs) of L. casei-immunized HIL extract against Staphylococcus aureus, Escherichia coli, and Salmonella species ranged from 100~200 µg/100 µL and no cytotoxicity to CaCo-2 and L929 cells were observed in the concentration range 100~40,000 µg/100 µL. Taken together, the present investigation demonstrates that L. casei-immunized HIL extract is a powerful natural antibiotic and preservative that can prevent contamination by Salmonella species.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Lee

Yun

Goo

2020

Insects

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“…The mobile phase conditions were modified from that described in a previous report [17], as follows: 25 mM AA-ACN (80:20, v/v) was used as solvent B in lieu of 20 mM potassium dihydrogen phosphate; the pH of the mobile phase was adjusted to 4.5 with PPA. The optimized mobile phase conditions were obtained to give the best resolution after comparing several mobile phases (data not shown).…”

Section: Optimization Of the Mobile Phase And Gradient Elution Modementioning

confidence: 99%

“…Under the optimized conditions, the gradient elution time was shortened to less than 5 min at a flow rate of 0.45 mL/ min. The total run time was set at 6 min; only half the time used in the previous report [17]. Consequently, successive and rapid separation of nine chemical preservatives was accomplished by optimizing analytical conditions, as shown in Fig.…”

Section: Optimization Of the Mobile Phase And Gradient Elution Modementioning

confidence: 99%

Simultaneous and rapid analysis of chemical preservatives in processed animal products by ultra-performance liquid chromatography

Park

Choi

et al. 2017

Food Sci Biotechnol

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An ultra-performance liquid chromatography-tunable ultraviolet method was optimized and validated for the simultaneous analysis of nine chemical preservatives in processed animal products. The limits of detection and quantification for the preservatives were within the ranges of 0.02-0.23 and 0.07-0.76 μg/mL, respectively. The relative standard deviations for intraday analyses of retention time and peak area were 0.00-0.23 and 0.03-2.93%, respectively, whereas, those for interday analyses were 0.67-2.30 and 2.12-5.37%, respectively. Of the nine preservatives spiked into six different animal products, dehydroacetic acid spiked into soft cheese exhibited the lowest recovery rate of 72.1 ± 0.36% at the lowest concentration (0.25 g/kg). Comparing data between UPLC and high-performance liquid chromatography with a 5% significance level, the t-statistic was 1.42. Moreover, sorbic acid was detected in 16 animal products (0.11-2.49 g/kg) when 278 products were analyzed for preservatives.

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“…Over the years various analytical techniques for the determination of parabens in different matrices have been developed, including gas-chromatography (GC) [ 9 , 15 , 16 ], high performance liquid chromatography (HPLC) [ 13 , 17 ], capillary zone electrophoresis (CZE) [ 4 ] and ultra performance liquid chromatography [ 18 , 19 ] amongst others. HPLC methods are the most widely used for the analysis and determination of parabens since techniques such as GC require prior derivatization of the compounds [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

In-Syringe Micro Solid-Phase Extraction Method for the Separation and Preconcentration of Parabens in Environmental Water Samples

2018

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In this study, a simple, rapid and effective in-syringe micro-solid phase extraction (MSPE) method was developed for the separation and preconcetration of parabens (methyl, ethyl, propyl and butyl paraben) in environmental water samples. The parabens were determined and quantified using high performance liquid chromatography and a photo diode array detector (HPLC-PDA). Chitosan-coated activated carbon (CAC) was used as the sorbent in the in-syringe MSPE device. A response surface methodology based on central composite design was used for the optimization of factors (eluent solvent type, eluent volume, number of elution cycles, sample volume, sample pH) affecting the extraction efficiency of the preconcentration procedure. The adsorbent used displayed excellent absorption performance and the adsorption capacity ranged from 227–256 mg g−1. Under the optimal conditions the dynamic linear ranges for the parabens were between 0.04 and 380 µg L−1. The limits of detection and quantification ranged from 6–15 ng L−1 and 20–50 ng L−1, respectively. The intraday (repeatability) and interday (reproducibility) precisions expressed as relative standard deviations (%RSD) were below 5%. Furthermore, the in-syringe MSPE/HPLC procedure was validated using spiked wastewater and tap water samples and the recoveries ranged between from 96.7 to 107%. In conclusion, CAC based in-syringe MSPE method demonstrated great potential for preconcentration of parabens in complex environmental water.

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UPLC-PDAD Analysis for Simultaneous Determination of Ten Synthetic Preservatives in Foodstuff

Cited by 13 publications

References 21 publications

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Antimicrobial Activity of an Extract of Hermetia illucens Larvae Immunized with Lactobacillus casei against Salmonella Species

Simultaneous and rapid analysis of chemical preservatives in processed animal products by ultra-performance liquid chromatography

In-Syringe Micro Solid-Phase Extraction Method for the Separation and Preconcentration of Parabens in Environmental Water Samples

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