Upregulated expression of CAP1 is associated with tumor migration and metastasis in hepatocellular carcinoma

Liu, Yanhua; Cui, Xiaopeng; Hu, Baoying; Lu, Cuihua; Huang, Xiaodong; Cai, Jing; He, Song; Lv, Liting; Xia, Cong; Liu, Guoliang; Zhang, Yixin; Ni, Runzhou

doi:10.1016/j.prp.2013.11.011

Cited by 29 publications

(23 citation statements)

References 33 publications

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“…Furthermore, CAP1 showed higher gene expression levels in both significant CAD and nonsignificant CAD groups compared to the CG. Overexpression of CAP1 was found in many types of cancers [16][17][18], and considering that cell motility is one of the pivotal roles of CAP proteins [9], it seems that overexpressed CAP1 has a role in tumour invasion and metastasis, which was associated with the poor outcome of patients [17,18]. However, CAP1 has never been evaluated in patients with CAD in the context of resistin receptor.…”

Section: Discussionmentioning

confidence: 99%

Association of adenylate cyclase‐associated protein 1 with coronary artery disease

Munjas

Sopić

Spasojević‐Kalimanovska

et al. 2017

Eur J Clin Investigation

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Section: Discussionmentioning

confidence: 99%

Association of adenylate cyclase‐associated protein 1 with coronary artery disease

Munjas

Sopić

Spasojević‐Kalimanovska

et al. 2017

Eur J Clin Investigation

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“…After knocking down its expression, the proliferation and migration of MDA-MB-231 cells was shown to decrease, inducing changes in morphology, which were associated with the arrangement of F-actin ( 25 ). Western blot analysis, real-time PCR and immunohistochemical analysis have been used to prove that CAP1 is overexpressed in hepatocellular carcinoma compared with adjacent non-cancerous liver tissues, and that it is positively associated with HCC cell metastasis ( 24 ). CAP1 overexpression has been shown to be significantly associated with lymph node status in esophageal squamous cell carcinoma.…”

Section: Discussionmentioning

confidence: 99%

“…Tissues with no staining were rated as 0, with a faint staining or moderate to strong staining in ≤25% of cells as 1, with moderate staining or strong staining in 25–50% of cells as 2, and strong staining in ≥50% of cells as 3. For statistical analysis, a score of <2 was counted as low expression, while a score of ≥2 was counted as overexpression, as previously described ( 24 ). When evaluating Ki-67 protein immunoreaction, staining was scored in a semi-quantitative manner.…”

Section: Methodsmentioning

confidence: 99%

“…On both ends of the actin filament, CAP1 rapidly translocates to the mitochondria upon treatment with agents that induce apoptosis ( 23 ). Studies have demonstrated that CAP1 is overexpressed in hepatocelluar carcinoma ( 24 ), breast cancer ( 25 ), lung cancer ( 26 ) and esophageal squamous cell carcinoma ( 27 ). However, to the best of our knowledge, no more information is available to date regarding the role of CAP1 in ovarian tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

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CAP1 is overexpressed in human epithelial ovarian cancer and promotes cell proliferation

Mao

Shu

Deng

et al. 2015

International Journal of Molecular Medicine

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Adenylate cyclase-associated protein 1 (CAP1) regulates both actin filaments and the Ras/cAMP pathway in yeast, and has been found play a role in cell motility and in the development of certain types of cancer. In the present study, we investigated CAP1 gene expression in human epithelial ovarian cancer (EOC). Western blot analysis and immunohistochemistry were performed using EOC tissue samples and the results revealed that CAP1 expression increased with the increasing grade of EOC. In the normal ovarian tissue samples however, CAP1 expression was barely detected. Using Pearson’s χ2 test, it was demonstrated that CAP1 expression was associated with the histological grade and Ki-67 expression. Kaplan-Meier analysis revealed that a higher CAP1 expression in patients with EOC was associated with a poorer prognosis. In in vitro experiments using HO-8910 EOC cells, the expression of CAP1 was knocked down using siRNA. The proliferation of the HO-8910 cells was then determined by cell cycle analysis and cell proliferation assay using the cell counting kit-8 and flow cytometry. The results revealed that the loss of CAP1 expression inhibited cell cycle progression. These findings suggest that a high expression of CAP1 is involved in the pathogenesis of EOC, and that the downregulation of CAP1 in tumor cells may be a therapeutic target for the treatment of patients with EOC.

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“…The N-terminal region of CAP binds to Cyr1 and is associated with Ras responsiveness in yeast (10)(11)(12). The C-terminal region of this protein binds monomeric actin with high affinity, and is necessary for normal cellular morphology (13) Previous research has shown that CAP1 is overexpressed in hepatocellular carcinoma (14), breast (15) and lung cancer (16), and esophageal squamous cell carcinoma (17). Yet, there is sparse research on the influence of CAP1 in gliomas.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of CAP1 and its significance in tumor cell proliferation, migration and invasion in glioma

et al. 2016

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Adenylate cyclase-associated protein 1 (CAP1), a protein related to the regulation of actin filaments and the Ras/cAMP pathway, is associated with tumor progression. Nevertheless, the expression level and effects of CAP1 in regards to glioma have not been reported. In the present study, we examined the expression of CAP1 in glioma and tumor adjacent normal brain tissues by tissue microarray and immunohistochemistry. Our results showed that CAP1 was overexpressed in glioma tissues in comparison with that noted in the tumor adjacent normal brain tissues and increased staining of CAP1 was found to be correlated with WHO stage. In addition, we discovered that knockdown of CAP1 by specific RNA interference markedly inhibited cell growth and caused downregulation of the proliferation markers, PCNA and cyclin A. We further demonstrated that knockdown of CAP1 inhibited cell metastatic abilities by downregulating N-cadherin and vimentin and upregulating E-cadherin. These findings revealed that CAP1 expression is markedly increased in human glioma and that downregulation of CAP1 in tumors may serve as a treatment for glioma patients.

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Upregulated expression of CAP1 is associated with tumor migration and metastasis in hepatocellular carcinoma

Cited by 29 publications

References 33 publications

Association of adenylate cyclase‐associated protein 1 with coronary artery disease

Association of adenylate cyclase‐associated protein 1 with coronary artery disease

CAP1 is overexpressed in human epithelial ovarian cancer and promotes cell proliferation

Overexpression of CAP1 and its significance in tumor cell proliferation, migration and invasion in glioma

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