Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma

Bo, Hao; Gong, Zhaojian; Zhang, Wenling; Li, Xiayu; Zeng, Yong; Liao, Qianjin; Pan, Chaoyun; Shi, Lei; Yu, Li; Jing, Yizhou; Tang, Ke; Li, Zheng; Zhou, Yanhong; Zhou, Ming; Xiang, Bo; Li, Xiaoling; Yang, Jianbo; Xiong, Wei; Li, Guiyuan; Zeng, Zhaoyang

doi:10.18632/oncotarget.4057

Cited by 200 publications

(192 citation statements)

References 46 publications

Supporting

Mentioning

183

Contrasting

Unclassified

Order By: Relevance

“…Knockdown of HNF1A-AS suppressed proliferation and migration of NPC cells [100]. AFAP1-AS1 knockdown increased AFAP1 protein expression, thereby inhibiting the NPC cell migration and invasive capability [101]. Recently, high-through put analysis revealed that lncRNA LOC100129148 was highly expressed in NPC.…”

Section: Oncogenic Lncrnasmentioning

confidence: 99%

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

Hann²

2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Nasopharyngeal carcinoma (NPC) is one of the most common cancers originating in the nasopharynx and occurring at high frequency in South-eastern Asia and North Africa. Long non-coding RNAs (lncRNAs) are a class of non-protein-coding RNA molecules and key regulators of developmental, physiological, and pathological processes in humans. Emerging studies have shown that lncRNAs play critical roles in tumorgenicity and cancer prognosis. With the development of deep sequencing analyses, an extensive amount of functional lncRNAs have been discovered in nasopharyngeal carcinoma tissues and cell lines. However, the roles and mechanisms of aberrantly expressed lncRNAs in the pathogenesis of NPC are not fully understood. In this review, we briefly illustrate the concept, identification, functional characterization, and summarize recent advancements of biological functions of lncRNAs with heterogeneous mechanistic characterization and their involvement in NPC. Then, we describe individual lncRNAs that have been associated with tumorgenesis, growth, invasion, cancer stem cell differentiation, metastasis, drug resistance and discuss the strategies of their therapeutic manipulation in NPC. We also review the emerging insights into the role of lncRNAs and their potential as biomarkers and therapeutic targets for novel treatment paradigms. Finally, we highlight the up-to-date of clinical information involving lncRNAs and future directions in the linking lncRNAs to potential gene therapies, and how modifications of lncRNAs can be exploited for prevention and treatment of NPC.

show abstract

Section: Oncogenic Lncrnasmentioning

confidence: 99%

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

Hann²

2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…30,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58] In this review, the related clinicopathological characteristics and molecular functions of this lncRNA in cancers are presented in Tables 1 and 2.…”

Section: Identification Of Afap1-as1mentioning

confidence: 99%

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Zhang

Xiao

et al. 2017

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…Recent studies have shown that AFAP1-AS1 promoted cells proliferation and invasion in nasopharyngeal carcinoma, hepatocellular carcinoma, esophageal squamous cell carcinoma, gallbladder cancer [17, 19, 22, 23]. To investigate roles played by AFAP1-AS1 in CCA, we first examined the expression of AFAP1-AS1 in all 4 CCA cell lines (QBC939, CCLP1, HuCC-T1 and RBE) and the nonmalignant cholangiocyte cell line BEC.…”

Section: Resultsmentioning

confidence: 99%

“…LncRNA CPS1-IT1 was observed to be upregulated in intrahepatic cholangiocarcinoma and knockdown of CPS1 CPS1-IT1 decreased the proliferation and indued the apoptosis of ICC-9810 cells [16]. LncRNA AFAP1-AS1 (actin filament associated protein 1 antisense RNA1), has been reported to be upregulated in esophageal adenocarcinoma, hepatocellular carcinoma, pancreatic ductal adenocarcinoma, and nasopharyngeal carcinoma [17-20]. However, the expression and detailed function of AFAP1-AS1 in CCA remains largely unclear and needs to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

Xu¹,

Zhou²,

Li³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Long non-coding RNAs (lncRNAs) have been shown to play important roles in a wide range of pathophysiological processes, including cancer progression. Our previous study has shown that AFAP1-AS1 was upregulated and acted as an oncogene in hepatocellular carcinoma. However, the expression and biological functions of lncRNA AFAP1-AS1 in intrahepatic cholangiocarcinoma (CCA) remains largely unknown. Methods: The expression level of AFAP1-AS1 was measured in 56 pairs of human cholangiocarcinoma tumor tissues and corresponding adjacent normal bile duct tissues. The correlation between AFAP1-AS1 and the clinicopathological features were evaluated by chi-square test. The effects of AFAP1-AS1 on CCA cells were determined by CCK-8 assay, clone formation assay, flow cytometry and transwell assay. Finally, to determine the effect of AFAP1-AS1 on tumor growth in vivo, AFAP1-AS1 knockdowned CCLP-1 cells were subcutaneously into nude mice to evaluate tumor growth. Results: In this study, we found that lncRNA AFAP1-AS1 was increased in CCA tissues and patients with high AFAP1-AS1 expression had a shorter overall survival. SiRNA-mediated AFAP1-AS1 knockdown significantly decreased cell proliferation of the CCA cells, with downregulation of C-myc and Cycling D1 in vitro. Furthermore, AFAP1-AS1 silencing inhibited cell migration partly due to decrease the expression of MMP-2 and MMP-9. In addition, CCLP-1 cells with AFAP1-AS1 knockdown were injected into nude mice to investigate the effect of AFAP1-AS1 on the tumorigenesis in vivo. Conclusions: Taken together, our findings suggested that AFAP1-AS1 might promote the CCA progression and provided a novel potential therapeutic target for CCA.

show abstract

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma

Cited by 200 publications

References 46 publications

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancers

Long Noncoding RNA AFAP1-AS1 Promoted Tumor Growth and Invasion in Cholangiocarcinoma

Contact Info

Product

Resources

About