Upregulation of long noncoding RNA <i>MEG3</i> inhibits the osteogenic differentiation of periodontal ligament cells

Liu, Yi; Zeng, Xuemin; Miao, Jie; Liu, Chunpeng; Wei, Fulan; Liu, Dongxu; Zheng, Zhong; Ting, Kang; Wang, Chunling; Guo, Jie

doi:10.1002/jcp.27248

Cited by 37 publications

(28 citation statements)

References 38 publications

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“…Studies in past several years also showed that the development of periodontitis is also accompanied by changed in expression patterns of a large set of lncRNAs [11]. Certain lncRNA, such ANCR and MEG3 regulate osteogenic differentiation to participate in the pathogenesis of periodontitis [12, 13]. LncRNA PTCSC3 has been characterized as a tumor suppressor [14], while its roles in other human diseases are unclear.…”

Section: Introductionmentioning

confidence: 99%

LncRNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) regulates the proliferation of human periodontal ligament stem cells and toll-like receptor 4 (TLR4) expression to improve periodontitis

Liu¹,

Zheng²,

Chen³

et al. 2019

BMC Oral Health

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Background LncRNA PTCSC3 is a known tumor suppressor, while its roles in other human diseases are unclear. Methods All participants were admitted by The second Affiliated Hospital, School of Medicine, Zhejiang University from January 2016 to January 2018. RT-qPCR, Vectors, cell transfection, In vitro cell proliferation assay and western blot were using to carry out the experiment. Results In this study we found that PTCSC3 was downregulated, while toll-like receptor 4 (TLR4) was upregulated in periodontal ligament stem cells (PDLSCs) isolated from periodontitis affected teeth that in PDLSCs isolated from healthy teeth. Expression levels of PTCSC3 and TLR4 were only significantly and inversely correlated in PDLSCs isolated from periodontitis affected teeth but not in PDLSCs isolated from healthy teeth. PTCSC3 overexpression led to the downregulation of TLR4 in PDLSCs isolated from periodontitis affected teeth, while TLR4 overexpression failed to significantly affect PTCSC3. PTCSC3 overexpression also led to the inhibited proliferation of periodontitis-affected PDLSCs. Conclusions Therefore, lncRNA PTCSC3 may regulate the proliferation of PDLSCs and TLR4 expression to improve periodontitis.

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Section: Introductionmentioning

confidence: 99%

LncRNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) regulates the proliferation of human periodontal ligament stem cells and toll-like receptor 4 (TLR4) expression to improve periodontitis

Liu¹,

Zheng²,

Chen³

et al. 2019

BMC Oral Health

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“…It has been shown that MEG3 can compete with BMP2 mRNA for interaction with hnRNPI. Therefore, MEG3 overexpression suppresses BMP2 levels and attenuates osteogenic differentiation in periodontal ligament cells (11).…”

Section: Roles Of Lncrna Meg3 In Osteogenic Differentiationmentioning

confidence: 99%

“…BMP2 is an important molecule responsible for regulation of osteogenesis (18). Modifications of BMP2 mRNA are associated with heterogeneous nuclear ribonucleoprotein I (hnRNPI), and the downregulation of hnRNPI can in turn decrease BMP2 protein expression levels (11). It has been shown that MEG3 can compete with BMP2 mRNA for interaction with hnRNPI.…”

Section: Roles Of Lncrna Meg3 In Osteogenic Differentiationmentioning

confidence: 99%

“…MEG3 expression is downregulated in several primary human tumors and tumor cell lines, and it may function as a novel tumor suppressor (4,8,9). An increasing number of studies have shown that MEG3 serves an important role in the development of osteogenic differentiation and thus regulates bone formation, metabolism, and regeneration (10,11). Additionally, dysregulation of MEG3 is associated with various bone diseases including bone tumors, joint degeneration, osteoporosis and rheumatoid arthritis (RA) (12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

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Long non‑coding RNA MEG3 is involved in osteogenic differentiation and bone diseases (Review)

Sun

Peng

et al. 2020

Biomed Rep

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Osteogenic differentiation originating from mesenchymal stem cells (MSCs) requires tight coordination of transcriptional factors, signaling pathways and biomechanical cues. Dysregulation of such reciprocal networks may influence the proliferation and apoptosis of MSCs and osteoblasts, thereby impairing bone metabolism and homeostasis. An increasing number of studies have shown that long non-coding (lnc)RNAs are involved in osteogenic differentiation and thus serve an important role in the initiation, development, and progression of bone diseases such as tumors, osteoarthritis and osteoporosis. It has been reported that the lncRNA, maternally expressed gene 3 (MEG3), regulates osteogenic differentiation of multiple MSCs and also acts as a critical mediator in the development of bone formation and associated diseases. In the present review, the proposed mechanisms underlying the roles of MEG3 in osteogenic differentiation and its potential effects on bone diseases are discussed. These discussions may help elucidate the roles of MEG3 in osteogenic differentiation and highlight potential biomarkers and therapeutic targets for the treatment of bone diseases. Contents 1. Introduction 2. Roles of lncRNA MEG3 in osteogenic differentiation 3. Roles of lncRNA MEG3 in bone diseases 4. Conclusion

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“…Wang and colleagues reported that lncRNA‐POIR expression was significantly up‐regulated in periodontitis tissues, and its abnormal expression was correlated with the osteogenesis of PDLCs mediated by NF‐kappa B/Wnt pathway, suggesting that lncRNA‐POIR may be an important molecular target in periodontitis 10 . Recently, Liu et al 11 indicated that overexpression of MEG3 inhibited cell viability, osteogenic differentiation, and increased cell apoptosis of periodontal ligament cells by down‐regulating BMP2 expression. To explore potential lncRNAs involved in the development of periodontitis, the lncRNA microarray profiles of gingival tissue from periodontitis patients and healthy controls were analyzed, and several differentially expressed lncRNAs were identified, such as lncRNA‐01126, KLLP, and FIFH.…”

Section: Introductionmentioning

confidence: 99%

LncRNA‐01126 inhibits the migration of human periodontal ligament cells through MEK/ERK signaling pathway

Zhu

Ding³

et al. 2020

J of Periodontal Research

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Background and Objective Accumulating findings revealed that long noncoding RNAs (lncRNAs) are crucial regulator molecules in the progression of periodontitis. This study aimed to investigate the biological roles and mechanisms of lncRNA‐01126 in the progression of periodontitis. Materials and Methods RT‐qPCR was used to detect the levels of lncRNA‐01126 in gingival tissues and human periodontal ligament cells (hPDLCs). Cell transfection experiments were performed to knock down or overexpress the level of lncRNA‐01126 in hPDLCs. Cell Counting Kit‐8, wound‐healing assay, transwell assay, and flow cytometric analysis were used to evaluate the function of lncRNA‐01126 in the progression of periodontitis. Finally, the signaling pathway was assessed by western blot. Results LncRNA microarray discovered that lncRNA‐01126 was the most significantly increased lncRNA in periodontitis patients. LncRNA‐01126 markedly increased in the gingival tissues of periodontitis mice and in the hPDLCs treated with lipopolysaccharide of Porphyromonas Gingivalis (LPS‐PG). Furthermore, in vitro experiments showed that lncRNA‐01126 dramatically suppressed the migration of hPDLCs through MEK/ERK signaling pathway. Conclusion LncRNA‐01126 plays a crucial role in inhibiting the migration of hPDLCs through MEK/ERK signaling pathway.

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Upregulation of long noncoding RNA MEG3 inhibits the osteogenic differentiation of periodontal ligament cells

Abstract: Upregulation of MEG3 inhibits the osteogenic differentiation of periodontal ligament cells by downregulating BMP2 expression.

Cited by 37 publications

References 38 publications

LncRNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) regulates the proliferation of human periodontal ligament stem cells and toll-like receptor 4 (TLR4) expression to improve periodontitis

LncRNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) regulates the proliferation of human periodontal ligament stem cells and toll-like receptor 4 (TLR4) expression to improve periodontitis

Long non‑coding RNA MEG3 is involved in osteogenic differentiation and bone diseases (Review)

LncRNA‐01126 inhibits the migration of human periodontal ligament cells through MEK/ERK signaling pathway

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