Uptake and metabolism of [ 14 C]rinderine and [ 14 C]retronecine in leaf-beetles of the genus Platyphora and alkaloid accumulation in the exocrine defensive secretions

Pasteels, Jacques; Theuring, Claudine; Windsor, Donald M.; Hartmann, Thomas

doi:10.1007/s000490300006

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…To ensure that metabolites in the insects are indeed sequestered from the host plants, feeding experiments with labelled compounds generally give the clearest evidence (e.g. Scudder and Meredith 1982b;Nahrstedt and Davis 1986;Lindigkeit et al 1997;Ehmke et al 1999;Narberhaus et al 2003;Pasteels et al 2003a).…”

Section: Discussionmentioning

confidence: 99%

Plant chemistry and insect sequestration

2009

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Most plant families are distinguished by characteristic secondary metabolites, which can function as putative defence against herbivores. However, many herbivorous insects of different orders can make use of these plant-synthesised compounds by ingesting and storing them in their body tissue or integument. Such sequestration of putatively unpalatable or toxic metabolites can enhance the insects' own defence against enemies and may also be involved in reproductive behaviour. This review gives a comprehensive overview of all groups of secondary plant metabolites for which sequestration by insect herbivores belonging to different orders has been demonstrated. Sequestered compounds include various aromatic compounds, nitrogen-containing metabolites such as alkaloids, cyanogenic glycosides, glucosinolates and other sulphurcontaining metabolites, and isoprenoids such as cardiac glycosides, cucurbitacins, iridoid glycosides and others. Sequestration of plant compounds has been investigated most in insects feeding or gathering on Apocynaceae s.l. (Apocynoideae, Asclepiaoideae), Aristolochiaceae, Asteraceae, Boraginaceae, Fabaceae and Plantaginaceae, but it also occurs for some gymnosperms and even lichens. In total, more than 250 insect species have been shown to sequester plant metabolites from at least 40 plant families. Sequestration predominates in the Coleoptera and Lepidoptera, but also occurs frequently in the orders Heteroptera, Hymenoptera, Orthoptera and Sternorrhyncha. Patterns of sequestration mechanisms for various compound classes and common or individual features occurring in different insect orders are highlighted. More research is needed to elucidate the specific transport mechanisms and the physiological processes of sequestration in various insect species.

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Section: Discussionmentioning

confidence: 99%

Plant chemistry and insect sequestration

2009

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“…PAs entering the hemolymph as tertiary alkaloid are detoxified by efficient glucosylation . So far, only species of leaf beetles from the neotropical genus Platyphora are known to store PAs exclusively as tertiary alkaloids , Pasteels et al 2003a. Tracer studies with P. boucardi revealed that this beetle seems to efficiently transport the poisonous tertiary PAs directly into its defensive glands.…”

Section: Discussionmentioning

confidence: 99%

Time course of pyrrolizidine alkaloid sequestration in Longitarsus flea beetles (Coleoptera, Chrysomelidae)

et al. 2004

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Several species of Longitarsus take up, metabolize and store pyrrolizidine alkaloids (PAs) from their host plants.In feeding experiments using radioactively labeled PAs of different types we examined the time course of the sequestration process in L. jacobaeae and L. aeruginosus. We found that adapted species efficiently store PAs for at least two weeks without major losses. During that time, there is virtually no change in the ratio of tertiary alkaloids to stored non-toxic Noxides, regardless of chemical form fed to the beetles. This implies a transient N-oxidation process where the alkaloids are only temporarily accessible to the enzyme. A dissection experiment with L. aeruginosus six days after uptake of labeled PAs showed that the tertiary alkaloids are not found in the hemolymph but are stored in the elytra and other body compartments. This conforms with earlier experiments that localized the enzyme's site of action in the hemolymph. Furthermore we show that different total alkaloid doses in the diet of L. jacobaeae and the potentially less adapted L. succineus do not affect the ratio of recovered N-oxides to tertiary molecules. Thus, the efficiency of the N-oxidizing enzyme is not dependent on the concentration of alkaloids offered.

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“…While certain compounds, such as amino acid and nicotinamide nucleotide derivatives, show no evident taxonomic association within the Chrysomelidae, three main classes of secretions appear to be specific to subtribes of beetles (see Pasteels et al 2003a). The Chrysomelina are characterized by isoxazolinone glucosides esterified by one or more nitropropanoic acid moieties, the Chrysolinina, by steroids (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…PAs are sequestered from plants or derived from plant PAs or necine bases Pasteels et al 2003b). Triterpene saponins are derived from β-amyrin (unpublished results), a common constituent of leaf waxes.…”

Section: Introductionmentioning

confidence: 99%

An ecdysteroid (22-acetyl-20-hydroxyecdysone) from the defense gland secretion of an insect: Chrysolina carnifex (Coleoptera: Chrysomelidae)

et al. 2003

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22-Acetyl-20-hydroxyecdysone was identified as a major component in the secretion of elytral and pronotal glands of Chrysolina carnifex, at an estimated concentration of 1.5 × 10 −1 M. This unexpected high concentration of an ecdysteroid in an exocrine secretion of an insect is discussed in terms of its potential defensive function, and in the light of steroid production in similar glands of closely related leaf beetles.

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Uptake and metabolism of [ 14 C]rinderine and [ 14 C]retronecine in leaf-beetles of the genus Platyphora and alkaloid accumulation in the exocrine defensive secretions

Cited by 10 publications

References 25 publications

Plant chemistry and insect sequestration

Plant chemistry and insect sequestration

Time course of pyrrolizidine alkaloid sequestration in Longitarsus flea beetles (Coleoptera, Chrysomelidae)

An ecdysteroid (22-acetyl-20-hydroxyecdysone) from the defense gland secretion of an insect: Chrysolina carnifex (Coleoptera: Chrysomelidae)

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