Uranium accumulation by Pseudomonas sp. EPS-5028

Marqués, Ana; Roca, Xavier; Simon-Pujol, Maria Dolores; Fusté, M. C.; Congregado, Francisco

doi:10.1007/bf00172734

Cited by 118 publications

(49 citation statements)

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“…Results revealed no significant difference in degree of adsorption or in qe values with respect to living cells, thus indicating that the observed ionic uptake is independent of cellular metabolic functions. Similar behaviour has been observed for Pseudomonas species (Marques et al 1991).…”

Section: Influence Of Bacterial Viabilitysupporting

confidence: 68%

“…Previous studies of microbial biosorption of Th 4÷ and UO 2÷ ions have been reported for Pseudomonas species (Strandberg et al 1981;Marques et al 1991) and for Rhizopus arrhizus (Tsezos and Volesky 1981). These authors showed that the efficiency of cellular uptake was relatively high, in certain cases exceeding that of common adsorbent materials.…”

Section: Introductionmentioning

confidence: 76%

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Adsorption of several actinide (Th, U) and lanthanide (La, Eu, Yb) ions by Mycobacterium smegmatis

Andrès

MacCordick

Hubert

1993

Appl Microbiol Biotechnol

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Adsorption measurements of several actinide [thorium (Th), uranium (U)] and lanthanide [lanthanum (La), europium (Eu), ytterbium (Yb)] cations by Mycobacterium smegmatis showed that sorption kinetics followed a three-phase pattern. For 5% (w/w) bacterial suspensions at pH 1, maximum cation bios0rption per gram dry biomass corresponded to 170 ~tmol Th 4+ and 187 ~tmol UO22+ . Adsorption of all cations studied obeyed the Brunauer-Emmett-Teller isotherm, which assumes multilayer binding at constant energy. Plots for the Scatchard model showed the existence of at least two types of cation complexation site, with strong and weak affinity and negative cooperation. Th 4+ was preferentially adsorbed with respect to the other cations, although all species appeared to compete for the same sites independently of bacterial viability. Adsorption of these cations was accompanied by partial release of magnesium from the cell wall, indicating that exchange reactions occurred at magnesium (Mg)-bonding sites.

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Section: Influence Of Bacterial Viabilitysupporting

confidence: 68%

Section: Introductionmentioning

confidence: 76%

Adsorption of several actinide (Th, U) and lanthanide (La, Eu, Yb) ions by Mycobacterium smegmatis

Andrès

MacCordick

Hubert

1993

Appl Microbiol Biotechnol

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“…Figure 8b shows that U(VI)-phosphate precipitates formed laths $40-70 nm long, but only 2-5 nm in width, while Figure 8c shows the corresponding EDS spectrum. Such laths have also been reported by Marques et al (1991) with a Pseudomonas sp. Copper peaks are the result of the copper grid used for mounting thin sections.…”

Section: Tem Hr-tem and Eds Analysismentioning

confidence: 61%

Multiple mechanisms of uranium immobilization by Cellulomonas sp. strain ES6

Sivaswamy

Boyanov

Peyton

et al. 2010

Biotech & Bioengineering

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Removal of hexavalent uranium (U(VI)) from aqueous solution was studied using a Gram-positive facultative anaerobe, Cellulomonas sp. strain ES6, under anaerobic, non-growth conditions in bicarbonate and PIPES buffers. Inorganic phosphate was released by cells during the experiments providing ligands for formation of insoluble U(VI) phosphates. Phosphate release was most probably the result of anaerobic hydrolysis of intracellular polyphosphates accumulated by ES6 during aerobic growth. Microbial reduction of U(VI) to U(IV) was also observed. However, the relative magnitudes of U(VI) removal by abiotic (phosphate-based) precipitation and microbial reduction depended on the buffer chemistry. In bicarbonate buffer, X-ray absorption fine structure (XAFS) spectroscopy showed that U in the solid phase was present primarily as a non-uraninite U(IV) phase, whereas in PIPES buffer, U precipitates consisted primarily of U(VI)-phosphate. In both bicarbonate and PIPES buffer, net release of cellular phosphate was measured to be lower than that observed in U-free controls suggesting simultaneous precipitation of U and PO₄³⁻. In PIPES, U(VI) phosphates formed a significant portion of U precipitates and mass balance estimates of U and P along with XAFS data corroborate this hypothesis. High-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDS) of samples from PIPES treatments indeed showed both extracellular and intracellular accumulation of U solids with nanometer sized lath structures that contained U and P. In bicarbonate, however, more phosphate was removed than required to stoichiometrically balance the U(VI)/U(IV) fraction determined by XAFS, suggesting that U(IV) precipitated together with phosphate in this system. When anthraquinone-2,6-disulfonate (AQDS), a known electron shuttle, was added to the experimental reactors, the dominant removal mechanism in both buffers was reduction to a non-uraninite U(IV) phase. Uranium immobilization by abiotic precipitation or microbial reduction has been extensively reported; however, the present work suggests that strain ES6 can remove U(VI) from solution simultaneously through precipitation with phosphate ligands and microbial reduction, depending on the environmental conditions. Cellulomonadaceae are environmentally relevant subsurface bacteria and here, for the first time, the presence of multiple U immobilization mechanisms within one organism is reported using Cellulomonas sp. strain ES6.

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“…The temperature affects the cell wall structure and its fidelity 28,29 . The enhanced bioremediation at higher temperature is caused by the increase in binding sites on biomass for heavy metals 30 . Figure 9 shows optimal biomass weight of 7.0 g for the maximum biosorption of copper and zinc, i.e.…”

mentioning

confidence: 99%

<i>In vitro</i> Compatibility of Fungi for the Biosorption of Zinc(II) and Copper(II) from Electroplating Effluent

et al. 2017

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The present study describes the potential of mixed fungal isolates, i.e. Aspergillus niger, Penicillium chrysogenum and Rhizopus oryzae for the removal of zinc(II) and copper(II) from aquatic environments. Capacity of mixed fungal biomass to adsorb Zn(II) and Cu(II) were studied in batch sorption experiments as bioremediators. Optimal conditions from contact time, pH, initial metal ion concentration and temperature for remediation of Zn(II) and Cu(II) were studied. Typically, the uptake of Zn(II) and Cu(II) rises with increasing pH up to 4.0. Optimal metal concentration was 150 mg/l when the maximum removal of copper and zinc was 69.5% and 30.3% respectively, was observed at initial metal concentration. Maximum uptake for metals was achieved after 15 min. The maximum biosorption for copper and zinc by selected fungi was achieved at 7.0 g of biosorbent. IR spectrum of three fungal species showed the presence of C=O groups, amine and amide N-H functional stretch.Keywords: Biosorption, copper, electroplating effluent, fungal isolates, zinc.WASTEWATER during electroplating activities involves poisonous heavy metals like chromium, mercury, zinc, nickel, cadmium, phosphorus and copper which build up in the food chain and cause different pathological states 1,2 . In recent years, association of different microbes has been used for wastewater treatment as bioremediators, as it was considered to have greater capability over pure and single isolates. The axenic cultures were devised to be mineralized rapidly and completely by the metabolic activity of different microorganisms 3 . The interactions between different heavy metals and microorganisms might be due to antagonistic, additive effects or synergic. These interactions might be multifaceted and distinguished which depends on the type of heavy metal ion and microbial species consortium. The collective effect of different heavy metals may be toxicity or growth stimulation in the same microbial consortium assorted from the additive effect of the single metal ions 4 . Fungi have diverse mechanisms for bioremediation of heavy metals, including metal uptake by cell-wall components, accumulation inside the cell or extracellular chelation by phytochelatins and metal lothioneins 5 . Waste samples were collected from a polluted water source near Al-Madina electroplating industry, Alipur Chattha, Pakistan, in sterilized and cleaned 1 l glass bottles with stopper. All samples were treated with 372 mg/l EDTA as the chelating agent to reduce toxicity in samples burdened with heavy metals. During examination, the samples were fresh, and were occurred immediately after return to the laboratory.The fungi were isolated by filtration of the electroplating waste for collection of fungal propagules using 0.45 m pore size filter paper 6 . The fungal spore suspension was prepared in 50 ml of saline solution, and 100 l of spore suspension was inoculated in sterilized petri dish with modified culture medium consisting of aureomycin (0.35 mg/l), glucose (10 g/l), rose bengal (0.035 mg/l), Cu...

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Uranium accumulation by Pseudomonas sp. EPS-5028

Cited by 118 publications

References 11 publications

Adsorption of several actinide (Th, U) and lanthanide (La, Eu, Yb) ions by Mycobacterium smegmatis

Adsorption of several actinide (Th, U) and lanthanide (La, Eu, Yb) ions by Mycobacterium smegmatis

Multiple mechanisms of uranium immobilization by Cellulomonas sp. strain ES6

<i>In vitro</i> Compatibility of Fungi for the Biosorption of Zinc(II) and Copper(II) from Electroplating Effluent

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