Urea enhances the photodynamic efficiency of methylene blue

Núñez, Silvia Cristina; Yoshimura, Tania Mateus; Ribeiro, Martha S.; Junqueira, Helena C.; Maciel, Cleiton; Coutinho-Neto, Maurı́cio D.; Baptista, Maurı́cio S.

doi:10.1016/j.jphotobiol.2015.03.018

Cited by 50 publications

(40 citation statements)

References 34 publications

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“…With the invention of lasers in the 1960s, laser light used for PDT was monochromatic, coherent radiation, and the treatment could be better defined using the optimum wavelength, a high energy density and light transmission through optical fibers [26, 34, 35]. …”

Section: Light Sourcementioning

confidence: 99%

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The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

et al. 2016

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In recent years there have been an increasing number of in vitro and in vivo studies that show positive results regarding antimicrobial photodynamic therapy (aPDT) used in dentistry. These include applications in periodontics, endodontics, and mucosal infections caused by bacteria present as biofilms. Antimicrobial photodynamic therapy is a therapy based on the combination of a non-toxic photosensitizer (PS) and appropriate wavelength visible light, which in the presence of oxygen is activated to produce reactive oxygen species (ROS). ROS induce a series of photochemical and biological events that cause irreversible damage leading to the death of microorganisms. Many light-absorbing dyes have been mentioned as potential PS for aPDT and different wavelengths have been tested. However, there is no consensus on a standard protocol yet. Thus, the goal of this review was to summarize the results of research on aPDT in dentistry using the PubMed database focusing on recent studies of the effectiveness aPDT in decreasing microorganisms and microbial biofilms, and also to describe aPDT effects, mechanisms of action and applications.

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Section: Light Sourcementioning

confidence: 99%

“…The high power laser provides the higher power indicated for surgery [20], increases the temperature within the pulp and around the periodontal ligament, which may lead to bone resorption or necrosis of the pulp and is more costly [34]. …”

Section: Light Sourcementioning

confidence: 99%

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

et al. 2016

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“…Observou-se que nos sistemas CBP/EtOH e HEC/H 2 O (Tabela 1) foram obtidos os melhores resultados na produção de EROs. Sugere-se que este aumento decorra de uma distribuição efetiva do ativo na malha do gel, minimizando sua dimerização (NUÑEZ et al, 2015), citado como um dos processos responsáveis por reduzir a eficiência do AM. A redução da eficiência de produção de EROs nas formulações ocorre mais extensivamente nas amostras incorporadas de ureia, o que sugere alguma interferência desta espécie no mecanismo de formação das espécies reativas.…”

Section: Resultsunclassified

Géis Poliméricos Contendo Azul De Metileno Como Novas Formulações Para Terapiafotodinâmica

Costa¹,

Pereira²,

Fontes³

et al. 2017

Anais Do 5º Encontro Brasileiro Para Inovação Terapêutica

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“…The interaction between phenothiazines and biological membranes occurs because there is amphipathic character of the molecules. The thiazine nucleus is relatively hydrophobic and its side chain can be hydrophilic and even positively charged depending on the pH of the environment (Homem- de-Mello et al, 2005de-Mello et al, , 2007Rodrigues et al, 2006;Perussi, 2007;Rodrigues, 2007;Bettanin et al, 2015;de Faria et al, 2015;Nuñez et al, 2015). Additionally, it has been shown that antipsychotic phenothiazine derivatives possess potent cytotoxicity against several types of tumor cells by triggering of apoptosis, with involvement of mitochondrial permeabilization (de Faria et al, 2015;de Mello et al, 2016;Wu et al, 2016;Chu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Competition Between Phenothiazines and BH3 Peptide for the Binding Site of the Antiapoptotic BCL-2 Protein

et al. 2020

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The study of proteins and mechanisms involved in the apoptosis and new knowledge about cancer's biology are essential for planning new drugs. Tumor cells develop several strategies to gain proliferative advantages, including molecular alterations to evade from apoptosis. Failures in apoptosis could contribute to cancer pathogenesis, since these defects can cause the accumulation of dividing cells and do not remove genetic variants that have malignant potential. The apoptosis mechanism is composed by proteins that are members of BCL-2 and cysteine-protease families. BH3-only peptides are the "natural" intracellular ligands of BCL-2 family proteins. On the other hand, studies have proved that phenothiazine compounds influence the induction of cellular death. To understand the characteristics of phenothiazines and their effects on tumoral cells and organelles involved in the apoptosis, as well as evaluating their pharmacologic potential, we have carried out computational simulation with the purpose of relating the structures of the phenothiazines with their biological activity. Since the tridimensional (3D) structure of the target protein is known, we have employed the molecular docking approach to study the interactions between compounds and the protein's active site. Hereafter, the molecular dynamics technique was used to verify the temporal evolution of the BCL-2 complexes with phenothiazinic compounds and the BH3 peptide, the stability and the mobility of these molecules in the BCL-2 binding site. From these results, the calculation of binding free energy between the compounds and the biological target was carried out. Thus, it was possible to verify that thioridazine and trifluoperazine tend to increase the stability of the BCL-2 protein and can compete for the binding site with the BH3 peptide.

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Urea enhances the photodynamic efficiency of methylene blue

Cited by 50 publications

References 34 publications

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

The application of antimicrobial photodynamic therapy (aPDT) in dentistry: a critical review

Géis Poliméricos Contendo Azul De Metileno Como Novas Formulações Para Terapiafotodinâmica

Competition Between Phenothiazines and BH3 Peptide for the Binding Site of the Antiapoptotic BCL-2 Protein

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