UreA, the major urea/H+ symporter in Aspergillus nidulans

Abreu, C. S. de; Sanguinetti, Manuel; Amillis, Sotiris; Ramón, Ana

doi:10.1016/j.fgb.2010.07.004

Cited by 38 publications

(62 citation statements)

References 63 publications

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“…The top five induced transporters were two putative urea permeases dur3-1 (UMAG_02625) and dur3-2 (UMAG_06253), two putative oligopeptide transporters (OPT) opt2 (UMAG_11057) and opt4 (UMAG_02387), and the candidate methylammonium permease (MEP) ump2 (UMAG_05889) (Supplemental Data Set 11, Figures 4A and 4D). In fungi, related transporter families are required for nitrogen utilization from peptides, urea and ammonium, respectively (ElBerry et al, 1993;Lorenz and Heitman, 1998;Hauser et al, 2001;Abreu et al, 2010;Hartmann et al, 2011;Navarathna et al, 2011;Dunkel et al, 2013), while OPTs can also mediate sulfur utilization by taking up glutathione (Bourbouloux et al, 2000). All five transporters are located in the magenta expression module ( Figure 2C), and are thus linked to biotrophy (Supplemental Data Set 11).…”

Section: Nitrogen Transportersmentioning

confidence: 99%

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

et al. 2018

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The corn smut fungus Ustilago maydis is a model organism for elucidating host colonization strategies of biotrophic fungi. Here we performed an in depth transcriptional profiling of the entire plant-associated development of U. maydis wild-type strains. In our analysis we focused on fungal metabolism, nutritional strategies, secreted effectors and regulatory networks. Secreted proteins were enriched in three distinct expression modules corresponding to stages on the plant surface, establishment of biotrophy and induction of tumors. These modules are likely the key determinants for U. maydis virulence. With respect to nutrient utilization, we observed that expression of several nutrient transporters was tied to these virulence modules rather than being controlled by nutrient availability. We show that oligopeptide transporters likely involved in nitrogen assimilation are important virulence factors. By measuring the intramodular connectivity of transcription factors, we identified the potential drivers for the virulence modules. While known components of the b-mating type cascade emerged as inducers for the plant surface and biotrophy module, we identified a set of yet uncharacterized transcription factors as likely responsible for expression of the tumor module. We demonstrate a crucial role for leaf tumor formation and effector gene expression for one of these transcription factors.

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Section: Nitrogen Transportersmentioning

confidence: 99%

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

et al. 2018

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“…When one gene is involved in more than one step, the complete entry is shown once and only the gene symbol and database entry is shown opposite additional steps. Experimental identification of each gene is described in: nadA (Ribard et al, 2003); hxA (Glatigny and Scazzocchio, 1995); hxB (Amrani et al, 1999(Amrani et al, , 2000; xanA (Cultrone et al, 2005); uaZ ; azgA (Cecchetto et al, 2004); uapA (Gorfinkiel et al, 1993); uapC (Diallinas et al, 1995); fcyB (Vlanti and Diallinas, 2008); furD (Amillis et al, 2007;Hamari et al, 2009); furA (Hamari et al, 2009); ureA (Abreu et al, 2010); uaX and uaW are described in this article. alX (Hamari et al, 2009) aaX and the putative ureidoglycolase gene were identified by similarity with the cognate genes of S. cerevisiae, confirmed for alX and aaX by their known chromosomal locations.…”

Section: Strainsmentioning

confidence: 99%

Completing the purine utilisation pathway of Aspergillus nidulans

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Oestreicher

Amillis

et al. 2011

Fungal Genetics and Biology

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“…Members of this urea/H + symporters group have been functionally characterized in the fungi Saccharomyces cerevisiae (ScDUR3 [9]), Paxillus involutus (PiDUR3 [10]), Aspergillus nidulans (UreA [11]) and Candida albicans (CaDUR3 [12]), and in the plants Arabidopsis thaliana (AtDUR3 [13,14]) and Oryza sativa (OsDUR3 [15]). They share 35–74% of pairwise sequence identity and most of them include 14–15 predicted α-helical transmembrane segments (TMS).…”

Section: Introductionmentioning

confidence: 99%

“…They share 35–74% of pairwise sequence identity and most of them include 14–15 predicted α-helical transmembrane segments (TMS). In A. nidulans , the urea transporter UreA has been identified as the only high-affinity urea transport system in this organism [11]. A second transport system may mediate facilitated diffusion at high urea concentrations (more than or equal to 3 mM) [11,16].…”

Section: Introductionmentioning

confidence: 99%

Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H ⁺ transporters in fungi and plants

et al. 2014

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We present the first account of the structure–function relationships of a protein of the subfamily of urea/H+ membrane transporters of fungi and plants, using Aspergillus nidulans UreA as a study model. Based on the crystal structures of the Vibrio parahaemolyticus sodium/galactose symporter (vSGLT) and of the Nucleobase-Cation-Symport-1 benzylhydantoin transporter from Microbacterium liquefaciens (Mhp1), we constructed a three-dimensional model of UreA which, combined with site-directed and classical random mutagenesis, led to the identification of amino acids important for UreA function. Our approach allowed us to suggest roles for these residues in the binding, recognition and translocation of urea, and in the sorting of UreA to the membrane. Residues W82, Y106, A110, T133, N275, D286, Y388, Y437 and S446, located in transmembrane helixes 2, 3, 7 and 11, were found to be involved in the binding, recognition and/or translocation of urea and the sorting of UreA to the membrane. Y106, A110, T133 and Y437 seem to play a role in substrate selectivity, while S446 is necessary for proper sorting of UreA to the membrane. Other amino acids identified by random classical mutagenesis (G99, R141, A163, G168 and P639) may be important for the basic transporter's structure, its proper folding or its correct traffic to the membrane.

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UreA, the major urea/H+ symporter in Aspergillus nidulans

Cited by 38 publications

References 63 publications

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

Completing the purine utilisation pathway of Aspergillus nidulans

Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H ⁺ transporters in fungi and plants

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UreA, the major urea/H+ symporter in Aspergillus nidulans

Cited by 38 publications

References 63 publications

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

The Biotrophic Development of Ustilago maydis Studied by RNA-Seq Analysis

Completing the purine utilisation pathway of Aspergillus nidulans

Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H + transporters in fungi and plants

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Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H ⁺ transporters in fungi and plants