Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

Izaguirre‐Mayoral, María Luisa; Lazarovits, George; Baral, Bikash

doi:10.1007/s11104-018-3674-x

Cited by 29 publications

(16 citation statements)

References 283 publications

(207 reference statements)

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“…Another effect that may show up is that salvage pathways become activated that use less energy and also often result in lower pool sizes of metabolites 40 . Further, many plant associated bacteria also contribute to purine and pyrimidine metabolism that may affect plant metabolite levels 41 . PGPR support plants but obviously also profit from plants and can thus also affect metabolism and levels of various metabolites.…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

El-Daim

Bejai

Meijer

2019

Sci Rep

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Abiotic stresses are main limiting factors for agricultural production around the world. Plant growth promoting rhizobacteria (PGPR) have been shown to improve abiotic stress tolerance in several plants. However, the molecular and physiological changes connected with PGPR priming of stress management are poorly understood. The present investigation aimed to explore major metabolic and molecular changes connected with the ability of Bacillus velezensis 5113 to mediate abiotic stress tolerance in wheat. Seedlings treated with Bacillus were exposed to heat, cold/freezing or drought stress. Bacillus improved wheat survival in all stress conditions. SPAD readings showed higher chlorophyll content in 5113-treated stressed seedlings. Metabolite profiling using NMR and ESI-MS provided evidences for metabolic reprograming in 5113-treated seedlings and showed that several common stress metabolites were significantly accumulated in stressed wheat. Two-dimensional gel electrophoresis of wheat leaves resolved more than 300 proteins of which several were differentially expressed between different treatments and that cold stress had a stronger impact on the protein pattern compared to heat and drought. Peptides maps or sequences were used for database searches which identified several homologs. The present study suggests that 5113 treatment provides systemic effects that involve metabolic and regulatory functions supporting both growth and stress management.

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Section: Discussionmentioning

confidence: 99%

Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

El-Daim

Bejai

Meijer

2019

Sci Rep

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“…The aggravation of existing agronomic problems due to climate change will be particularly important for the Mediterranean basin [12]. Under stressful environmental conditions (e.g., water deficit, nitrogen-deficient soils) the interactions established between ureidic legume species such as Phaseolus vulgaris L. and soil bacteria will mediate crop productivity [13]. As a rhizobial, nodulated, N 2 -fixing legume, the symbiotic relationships of Actinobacteria, Bacteroidetes and Firmicutes facilitate nutrient uptake and seeding under challenging environments.…”

Section: Introductionmentioning

confidence: 99%

Disclosing the Nutritional Quality Diversity of Portuguese Common Beans—The Missing Link for Their Effective Use in Protein Quality Breeding Programs

et al. 2021

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The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions’ nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap, a representative collection (106 accessions) was cropped under two contrasting environments (traditional versus heat stress) and evaluated in terms of nutritional quality by near-infrared spectroscopy. Protein quality was assessed, under the stressful environment, considering the individual amino acid contents and the activity of trypsin inhibitors through mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. On top of strong genotypic control, the nutritional composition (protein, fat, fiber, moisture and ash) was also highly influenced by the environment and by genotype × environment interaction, with a clear nutritional quality ranking change for the accessions in heat stress conditions. Classified into three clusters, the accessions from the cluster with the highest individual amino acid and protein contents also showed higher trypsin inhibitor activity (TIA). Since different levels of TIA had no translation into contrasting protein digestibility, breeders focusing on common beans’ protein quality improvement, especially under challenging warming climate conditions, may take advantage of this group of accessions.

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“…Triuret metabolic genes interestingly come from organisms with genera synonymous with plant-associated bacteria and fungi (mycorrhizal). A possible connection with triuret and plants is the role of uric acid metabolism as the main storage of nitrogen in plants and in pathogen defense ( 29 , 30 , 31 ). In plant nodules, uric acid is formed from the assimilation of nitrogen produced by bacterial endosymbionts fixing atmospheric nitrogen (N 2 ), which is then transported through the rest of the plant as allantoin, a uric acid metabolite ( 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Discovery of an ultraspecific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway

Tassoulas

Elias

Wackett

2021

Journal of Biological Chemistry

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Triuret (carbonyldiurea) is an impurity found in industrial urea fertilizer (<0.1% w/w) that is applied, worldwide, around 300 million pounds each year on agricultural lands. In addition to anthropogenic sources, endogenous triuret has been identified in amoeba and human urine, the latter being diagnostic for hypokalemia. The present study is the first to describe the metabolic breakdown of triuret, which funnels into biuret metabolism. We identified the gene responsible for triuret decomposition (trtA) in bacterial genomes, clustered with biuH, that encodes biuret hydrolase and has close protein sequence homology. TrtA is a member of the isochorismatase-like hydrolase protein family (IHL), similarly to BiuH, and has a catalytic efficiency (kcat/KM) of 6 x 105 (M-1s-1), a KM for triuret of 20 μM, and exquisite substrate specificity. Indeed, TrtA has four orders of magnitude less activity with biuret. Crystal structures of TrtA in apo and holo form were solved and compared to the BiuH structure. The high substrate selectivity was found to be conveyed by second shell residues around each active site. Mutagenesis of residues conserved in TrtA to the alternate consensus found in BiuHs revealed residues critical to triuret hydrolase activity but no single mutant evolved more biuret activity and likely a combination of mutations is required to interconvert between TrtA, BiuH functions. TrtA-mediated triuret metabolism is relatively rare in recorded genomes (1-2%), but is largely found in plant-associated, nodulating and endophytic bacteria. This study suggests functions for triuret hydrolase in certain eukaryotic intermediary processes and prokaryotic intermediary or biodegradative metabolism

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Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

Cited by 29 publications

References 283 publications

Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

Disclosing the Nutritional Quality Diversity of Portuguese Common Beans—The Missing Link for Their Effective Use in Protein Quality Breeding Programs

Discovery of an ultraspecific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway

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