Urethral reconstruction using an amphiphilic tissue-engineered autologous polyurethane nanofiber scaffold with rapid vascularization function

Niu, Yuqing; Liu, Guochang; Chen, Chuangbi; Fu, Ming; Fu, Wen; Zhang, Zhao; Xia, Hongmei; Stadler, Florian J.

doi:10.1039/c9bm01911a

Cited by 26 publications

(33 citation statements)

References 38 publications

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“…The surface properties of biomaterial scaffolds, such as hydrophilicity, softness, roughness, and chemical function, are critical to guide macrophage immune response [ 29 – 31 ]. Cell adhesion and diffusion is the first step in the interaction between cells and biomaterial scaffolds, which may regulate the behavior of immune cells, including polarization.…”

Section: Resultsmentioning

confidence: 99%

“…These results indicate that M2 phenotype macrophages play a crucial role in the homing process of endogenous urothelial progenitor cells, which is consistent with the published reports that biological scaffolds with appropriate biophysical and biochemical cues can polarize macrophages to M2 phenotype at the wound site, to achieve the state of promoting regeneration [ 20 , 34 , 43 ]. It is well known that in situ tissue regeneration depends on the migration and proliferation of endogenous progenitor stem cells to the wound site [ 29 , 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The animals were divided into 2 groups, each with 6 puppies. As described previously, [ 29 ] general anesthesia was induced and maintained with 45 mg/kg sodium pentobarbital (P0500000, Guangzhou pharmaceutical Medicine Co., Ltd.). Surgical disinfection was performed with Iodophor and 70 % ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…As described previously, [ 29 ] the animals were subjected to general anesthesia for macroscopic evaluation and monthly urinary cystourethrography (meglumine diatrizoate, M861408, 200 mg/mL, Guangzhou pharmaceutical Medicine Co., Ltd.). All images were collected with a Luminos Digital Radiography system (Allura Xper FD20, Philips, Netherlands).…”

Section: Methodsmentioning

confidence: 99%

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HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages

Niu

Stadler

et al. 2021

J Nanobiotechnol

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In situ tissue engineering utilizes the regenerative potential of the human body to control cell function for tissue regeneration and has shown considerable prospect in urology. However, many problems are still to be understood, especially the interactions between scaffolds and host macrophages at the wound site and how these interactions direct tissue integration and regeneration. This study was designed to evaluate the efficacy of hyaluronic acid (HA) functionalized collagen nanofibers in modulating the pro-healing phenotype expression of macrophages for urethral regeneration. Tubular HA-collagen nanofibers with HA-coating were prepared by coaxial electrospinning. The formation of a thin HA-coating atop each collagen nanofiber endowed its nanofibrous mats with higher anisotropic wettability and mechanical softness. The macrophages growing on the surface of HA-collagen nanofibers showed an elongated shape, while collagen nanofibers’ surface exhibited a pancake shape. Immunofluorescence and ELISA analysis showed that elongation could promote the expression of M2 phenotype marker and reduce the secretion of inflammatory cytokines. In vivo experiments showed that tubular HA-collagen nanofibers significantly facilitate male puppy urethral regeneration after injury. In the regenerated urethra bridged by tubular HA-collagen nanofibers, anti-inflammatory M2 macrophages are recruited to the surface of the scaffold, which can promote angiogenesis and endogenous urothelial progenitor cell proliferation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages

Niu

Stadler

et al. 2021

J Nanobiotechnol

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“…To the best of our knowledge, one of the key factors in reconstructing urethra is adequate blood supply and fast angiogenesis in implanted graft. Vascular density in the urethral cavity plays a central role in determining the fate of tissue repair [26,27]. Insu cient blood supply inevitably results in shrinkage of grafts and brosis formation.…”

Section: Discussionmentioning

confidence: 99%

Tubularized Urethral Reconstruction Using Everted Saphenous Vein Graft in A Beagle Model

Shen

2020

Preprint

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Background: The repair of anterior urethral stricture and hypospadias require urethral reconstruction. We conduct the study to investigate the efficacy of anterior urethral reconstruction using everted saphenous vein graft in tubular fashion. Methods: Twelve male beagles were randomly divided into three groups: experimental group( n=5), control group( n=5) and normal group( n=2). A 3 cm defect in anterior urethra was created. Autologous saphenous vein graft was harvested. In experimental group, urethral defect was replaced by everted saphenous vein graft in tubular fashion. In control group, urethral reconstruction was performed using uneverted saphenous vein graft. At 6 months postoperatively, beagles in all groups received retrograde urethrography to evaluate urethral patency and were killed for histological examination. Results: Retrograde urethrography showed the urethral caliber of beagles in experimental group were similar to those of normal. Ether urethral stricture or fistula were detected in all animals in control group. In experimental group, the everted saphenous vein graft completely integrated into the urethra. The reconstructed urethra had a wide lumen and was completely covered by urothelium. The subepithelial collagen and muscle fibers were well formed and highly organized. Everted saphenous vein graft had a high ability of neovascularization. In control group, the reconstructed segment had a fibrotic urethral lumen in which urothelium was not intact. And there were few newly formed capillaries. Conclusions: Everted saphenous vein graft can be used as tubular substitute material for anterior urethral strictures and hypospadias.

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A Modified Small Intestinal Submucosa Patch with Multifunction to Promote Scarless Repair and Reinvigoration of Urethra

Huang

Liu

et al. 2023

Adv Healthcare Materials

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To reconstruct and restore the functions of the male urethra is a challenging task for urologists. The acellular matrix graft currently used in the clinics is mono‐functional and may cause a series of complications including stricture, fibrosis, and stone formation. As a result, such graft materials cannot meet the increasing demand for multifunctionality in the field of urethral tissue engineering. In this context, a multifunctional urethral patch is designed for the repair of urethral defects by mixing protocatechualdehyde (PCA) with small intestinal submucosa (SIS) under an alkalin condition to allow cross linking. As shown, the PCA/SIS patch possesses excellent biocompatibility, antioxidant activity, and anti‐inflammatory property. More importantly, this patch can remarkably promote the adhesion, proliferation, and directional extension of rabbit bladder epithelial mucous cells (R‐EMCs) as well as rabbit bladder smooth muscle cells (R‐SMCs), and upregulate the expression of cytokeratin in the EMCs and contractile protein in the SMCs in vitro. In vivo experiments also confirm that the PCA/SIS patch can significantly enhance scarless repair of urethral defects in rabbits by facilitating smooth muscle regeneration, reducing excessive collagen deposition, and accelerating re‐epithelialization and neovascularization. Taken together, the newly developed multifunctional PCA/SIS patch provides a promising candidate for urethral regeneration.

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Urethral reconstruction using an amphiphilic tissue-engineered autologous polyurethane nanofiber scaffold with rapid vascularization function

Abstract: We report the efficient application of a well-layered tubular amphiphilic nanofiber of a polyurethane copolymer (PU-ran) for the regulation the phenotypic expression of epithelial cells (ECs) and smooth muscle cells (SMCs) for vascularized urethral reconstruction.

Cited by 26 publications

References 38 publications

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages

Tubularized Urethral Reconstruction Using Everted Saphenous Vein Graft in A Beagle Model

A Modified Small Intestinal Submucosa Patch with Multifunction to Promote Scarless Repair and Reinvigoration of Urethra

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