Urinary 2,5-hexanedione in workers exposed to n-hexane: influence of the sample treatment

2,5-Hexanedione (2,5-HD) is the most important metabolite of n-hexane and methyl ethyl ketone in human urine. Urinary 2,5-HD is used as a biomarker for biological monitoring of workers exposed to n-hexane. A simple method using headspace solid-phase microextraction (HS-SPME) and gas chromatography (GC) equipped with a flame-ionization detector (FID) was developed. The parameters that affect the HS-SPME-GC-FID process were optimized (i.e., fiber coating, sample volume, adsorption and heating time, salt addition, and extraction temperature). The assay presented linearity in the range of 0.075 to 20.0 mg/L, precision (coefficient of variation < 7.0%), and detection limit of 0.025 mg/L for 2,5-HD in urine. The method was successfully applied to the analysis of 2,5-HD in urine samples from eight workers occupationally exposed to n-hexane in shoemaker's glue.

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Electrophysiological Studies of Shoemakers Exposed to Sub‐TLV Levels of n‐hexane

Neghab

Soleimani

Khamoushian

2012

Journal of Occupational Health

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Sciences, Iran-Background and Objectives: Exposure to n-hexane, a neurotoxic solvent, has been associated with sensorimotor polyneuropathy, both in occupationally exposed workers and in glue-sniffing addicts. The present study was carried out to ascertain whether exposure to sub-TLV levels of n-hexane was associated with electrophysiological abnormalities and to determine if these possible abnormalities have any correlations with parameters such as the biological exposure index (BEI) of this neurotoxic chemical, workers' TWA exposure to n-hexane and/or duration of employment. Materials and Methods: Twenty-seven asymptomatic male workers from 6 shoemaking workshops were studied and compared with a group of 20 age-and sexmatched normal controls with no history of exposure to any neurotoxic agent. They underwent physical examinations as well as conventional needle electromyographic examinations and sensory and motor nerve conduction studies of upper and lower extremities. The TWA exposure to n-hexane and urinary concentration of free 2,5-hexanedione were also determined. Data were analyzed using version 16.0 of the SPSS/PC statistical package. Results: The TWA exposure to n-hexane was estimated to be 83.2 mg/m 3 . Electrophysiological studies showed that the amplitudes of sensory nerve action potential (SAP) for median and sural nerves were significantly lower in exposed subjects than in unexposed normal controls. Additionally, a significant correlation was found between these

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Urinary 2,5-hexanedione in workers exposed to n-hexane: influence of the sample treatment

Cited by 5 publications

References 13 publications

Selective Determination of n-Hexane and Methyl Ethyl Ketone (MEK) in Urine by Magnetic-Silica Aerogel-Based Molecularly Imprinted Polymers (MIPs) with Gas Chromatography – Flame Ionization Detection (GC-FID)

Selective Determination of n-Hexane and Methyl Ethyl Ketone (MEK) in Urine by Magnetic-Silica Aerogel-Based Molecularly Imprinted Polymers (MIPs) with Gas Chromatography – Flame Ionization Detection (GC-FID)

Determination of 2,5-Hexanedione in Urine by Headspace Solid-Phase Microextraction and Gas Chromatography

Electrophysiological Studies of Shoemakers Exposed to Sub‐TLV Levels of n‐hexane

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