Urinary excretion patterns of cyclic AMP and cyclic GMP following thermal injury

Wood, Peter; Coombes, E J; Pao, G.; Batstone, G.F.

doi:10.1016/0009-8981(81)90250-3

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Among these terms, GPCR activity and cyclin nucleotide degradation activity has their associated genes more highly expressed in 48 hpb condition (T3 in burn). Cyclic adenosine monophosphate (cAMP), as one type of cyclic nucleotides, is known to be at high levels in the urine of severe burn patients that gradually drops to a level closer to that in mild burn patients (32). It acts downstream of prostaglandin E2 (PGE2) to suppress the activation and growth of T-lymphocytes and granulocyte-macrophage progenitors (33, 34).…”

Section: Resultsmentioning

confidence: 99%

Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

Hou,

Khatri,

Rindy

et al. 2024

Preprint

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Neutrophils accumulate early in tissue injury. However, the cellular and functional heterogeneity of neutrophils during homeostasis and in response to tissue damage remains unclear. Here, we use larval zebrafish to understand neutrophil responses to thermal injury. Single-cell transcriptional mapping of myeloid cells during a 3-day time course in burn and control larvae revealed distinct neutrophil subsets and their cell-cell interactions with macrophages across time and conditions. The trajectory formed by three zebrafish neutrophil subsets resembles human neutrophil maturation, with varying transition patterns between conditions. Through ligand-receptor cell-cell interaction analysis, we found neutrophils communicate more in burns in a pathway and temporal manner. Finally, we identified the correlation between zebrafish myeloid signatures and human burn severity, establishing GPR84+ neutrophils as a potential marker of early innate immune response in burns. This work builds the molecular foundation and a comparative single-cell genomic framework to identify neutrophil markers of tissue damage using model organisms.

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Section: Resultsmentioning

confidence: 99%

Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

Hou,

Khatri,

Rindy

et al. 2024

Preprint

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Overnight urine growth hormone, cortisol and adenosine 3′ 5′ cyclic monophosphate excretion in children with chronic asthma treated with inhaled beclomethasone dipropionate

Zeitlin

Wood

Evans

et al. 1993

Respiratory Medicine

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Overnight urine samples were obtained from 34 asthmatic children, 24 of whom were receiving inhaled beclomethasone dipropionate (BDP), and 30 controls. The urine volume of the children receiving inhaled steroids was significantly greater than that of the other asthmatic children and of the controls (P < 0.05). Urine growth hormone was within the normal range for all of the subjects and there was no demonstrable relationship between urine growth hormone and height or height standard deviation score. Urine steroid output was significantly reduced in the BDP receiving group when the results were expressed in U l-1 but there was no difference between the groups when the results were expressed per specimen. Urine adenosine 3' 5' cyclic monophosphate (cAMP) results were similar for all groups. We conclude that use of BDP increases overnight urine volume but, in our study, does not appear to influence the output of urine cortisol. Urine free cortisol measurements may not be a very sensitive tool for the detection of small changes in endogenous steroid production. The use of BDP does not adversely affect the output of urine growth hormone.

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Single-cell Transcriptional Landscape of Temporal Neutrophil Response to Burn Wound in Larval Zebrafish

Hou,

Khatri,

Rindy

et al. 2024

The Journal of Immunology

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Neutrophils accumulate early in tissue injury. However, the cellular and functional heterogeneity of neutrophils during homeostasis and in response to tissue damage remains unclear. In this study, we use larval zebrafish to understand neutrophil responses to thermal injury. Single-cell transcriptional mapping of myeloid cells during a 3-d time course in burn and control larvae revealed distinct neutrophil subsets and their cell–cell interactions with macrophages across time and conditions. The trajectory formed by three zebrafish neutrophil subsets resembles human neutrophil maturation, with varying transition patterns between conditions. Through ligand–receptor cell–cell interaction analysis, we found that neutrophils communicate more in burns in a pathway and temporal manner. Finally, we identified the correlation between zebrafish myeloid signatures and human burn severity, establishing GPR84+ neutrophils as a potential marker of early innate immune response in burns. This work builds a comparative single-cell transcriptomic framework to identify neutrophil markers of tissue damage using model organisms.

show abstract

Urinary excretion patterns of cyclic AMP and cyclic GMP following thermal injury

Cited by 3 publications

References 18 publications

Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

Overnight urine growth hormone, cortisol and adenosine 3′ 5′ cyclic monophosphate excretion in children with chronic asthma treated with inhaled beclomethasone dipropionate

Single-cell Transcriptional Landscape of Temporal Neutrophil Response to Burn Wound in Larval Zebrafish

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